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Kidney Organogenesis pp 163–167Cite as

Time-Lapse Technologies and 4D Imaging of Kidney Development

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1926))

Abstract

Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions—3D and the time.

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Acknowledgments

This work was supported financially by the Suomen Akatemia (Academy of Finland) (206038, 121647, 250900, 260056; Centre of Excellence grant 2012-2017 251314), Munuaissäätiö—Finnish Kidney and Liver Association, the Sigrid Juséliuksen Säätiö, Novo Nordisk, Syöpäjärjestöt (Cancer Society of Finland), the European Community’s Seventh Framework Programme (FP7/2007-2013; grant FP7-HEALTH-F5-2012-INNOVATION-1 EURenOmics 305608), and H2020 Marie Skłodowska-Curie Actions Innovative Training Network “RENALTRACT” Project ID 642937.

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Authors and Affiliations

  1. Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland

    Ulla Saarela & Ilya Skovorodkin

  2. Laboratory of Developmental Biology, Biocenter Oulu, InfoTech, Oulu, Finland

    Ulla Saarela & Ilya Skovorodkin

  3. Oulu Center for Cell Matrix Research, Oulu, Finland

    Ulla Saarela & Ilya Skovorodkin

Authors
  1. Ulla Saarela
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  2. Ilya Skovorodkin
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Corresponding authors

Correspondence to Ulla Saarela or Ilya Skovorodkin .

Editor information

Editors and Affiliations

  1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

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Saarela, U., Skovorodkin, I. (2019). Time-Lapse Technologies and 4D Imaging of Kidney Development. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9021-4_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9020-7

  • Online ISBN: 978-1-4939-9021-4

  • eBook Packages: Springer Protocols

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