Abstract
Although plant organ segments bend in response to environmental stimuli such as gravity and light, they stop bending and subsequently straighten during the course of tropic responses. The straightening phenomenon can clearly be observed by setting the bent organs under microgravity and dark conditions. It has recently become clear that the straightening mechanism requires the activity of the actin-myosin XI cytoskeleton. A clinostat device makes it possible to simulate microgravity conditions by counteracting the Earth’s unilateral gravitational pull. Here, we describe a method for assessing the straightening ability of organs by clinostat analysis using Arabidopsis thaliana inflorescence stems of actin and myosin xi mutants as examples.
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References
Stankovic B, Volkmann D, Sack FD (1998) Autotropism, automorphogenesis, and gravity. Physiol Plant 102:328–335
Iino M (2006) Toward understanding the ecological functions of tropisms: interactions among and effects of light on tropisms. Curr Opin Plant Biol 9:89–93
Okamoto K, Ueda H, Shimada T, Tamura K, Kato T, Tasaka M, Morita TM, Hara-Nishimura I (2015) Regulation of organ straightening and plant posture by an actin–myosin XI cytoskeleton. Nat Plants 1:15031
Ueda H, Tamura K, Hara-Nishimura I (2015) Functions of plant-specific myosin XI: from intracellular motility to plant postures. Curr Opin Plant Biol 28:30–38
Deeks MJ, Rodrigues C, Dimmock S, Ketelaar T, Maciver SK, Malho R, Hussey PJ (2007) Arabidopsis CAP1 – a key regulator of actin organisation and development. J Cell Sci 120:2609–2618
Kandasamy MK, McKinney EC, Meagher RB (2009) A single vegetative actin isovariant overexpressed under the control of multiple regulatory sequences is sufficient for normal Arabidopsis development. Plant Cell 21:701–718
Kato T, Morita MT, Tasaka M (2010) Defects in dynamics and functions of actin filament in Arabidopsis caused by the dominant-negative actin fiz1-induced fragmentation of actin filament. Plant Cell Physiol 51:333–338
van der Honing HS, Kieft H, Emons AM, Ketelaar T (2012) Arabidopsis VILLIN2 and VILLIN3 are required for the generation of thick actin filament bundles and for directional organ growth. Plant Physiol 158:1426–1438
Lanza M, Garcia-Ponce B, Castrillo G, Catarecha P, Sauer M, Rodriguez-Serrano M, Páez-García A, Sánchez-Bermejo E, Mohan TC, del Puerto YL et al (2012) Role of actin cytoskeleton in brassinosteroid signaling and in its integration with the auxin response in plants. Dev Cell 22:1275–1285
Peremyslov VV, Mockler TC, Filichkin SA, Fox SE, Jaiswal P, Makarova KS, Koonin EV, Dolja VV (2011) Expression, splicing, and evolution of the myosin gene family in plants. Plant Physiol 155:1191–1204
Nakamura M, Toyota M, Tasaka M, Morita MT (2015) Live cell imaging of cytoskeletal and organelle dynamics in gravity-sensing cells in plant gravitropism. Methods Mol Biol 1309:57–69
Acknowledgments
This work was supported by the Grants-in-Aid for Scientific Research to I.H-N. (no. 15H05776) and to H.U. (nos. 15KT0151 and 16K07397) from the Japan Society for the Promotion of Science (JSPS).
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Ueda, H., Hara-Nishimura, I. (2019). How to Investigate the Role of the Actin-Myosin Cytoskeleton in Organ Straightening. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_18
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DOI: https://doi.org/10.1007/978-1-4939-9015-3_18
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