Abstract
Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Magni F, Sarto C, Ticozzi D, Soldi M, Bosso N, Mocarelli P, Kienle MG (2006) Proteomic knowledge of human aquaporins. Proteomics 6:5637–5649
Day RE, Kitchen P, Owen DS, Bland C, Marshall L, Conner AC et al (2014) Human aquaporins: regulators of transcellular water flow. Biochim Biophys Acta 1840:1492–1506. https://doi.org/10.1016/j.bbagen.2013.09.033
Alam J, Choi YS, Koh JH, Kwok SK, Park SH, Song YW et al (2017) Detection of autoantibodies against Aquaporin-1 in the sera of patients with primary Sjögren’s syndrome. Immune Netw 17:103–109. https://doi.org/10.4110/in.2017.17.2.103
Tzartos JS, Stergiou C, Kilidireas K, Zisimopoulou P, Thomaidis T, Tzartos SJ (2013) Anti-aquaporin-1 autoantibodies in patients with neuromyelitis optica spectrum disorders. PLoS One 8:e74773. https://doi.org/10.1371/journal.pone.0074773
Lennon VA, Kryzer TJ, Pittock SJ, Verkman AS, Hinson SR (2005) IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J Exp Med 202:473–477
Alam J, Koh JH, Kim N, Kwok SK, Park SH, Song YW et al (2016) Detection of autoantibodies against aquaporin-5 in the sera of patients with primary Sjögren’s syndrome. Immunol Res 64:848–856. https://doi.org/10.1007/s12026-016-8786-x
Wingerchuk DM, Banwell B, Bennett JL, Cabre P, Carroll W, Chitnis T et al (2015) International consensus diagnostic criteria for neuromyelitis optica spectrum disorders. Neurology 85:177–1789. https://doi.org/10.1212/WNL.0000000000001729
Waters PJ, Pittock SJ, Bennett JL, Jarius S, Weinshenker BG, Wingerchuk DM (2014) Evaluation of aquaporin-4 antibody assays. Clin Exp Neuroimmunol 5:290–303
Sánchez Gomar I, Díaz Sánchez M, Uclés Sánchez AJ, Casado Chocán JL, Suárez-Luna N, Ramírez-Lorca R et al (2016) Comparative analysis for the presence of IgG anti-aquaporin-1 in patients with NMO-spectrum disorders. Int J Mol Sci 17. https://doi.org/10.3390/ijms1708119
Ishikawa Y, Eguchi T, Skowronski MT, Ishida H (1998) Acetylcholine acts on M3 muscarinic receptors and induces the translocation of aquaporin5 water channel via cytosolic Ca2+ elevation in rat parotid glands. Biochem Biophys Res Commun 245:835–840
Yang F, Kawedia JD, Menon AG (2003) Cyclic AMP regulates aquaporin 5 expression at both transcriptional and post-transcriptional levels through a protein kinase A pathway. J Biol Chem 278:32173–32180
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Alam, J., Jeon, S., Choi, Y. (2019). Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_6
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8949-2_6
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8948-5
Online ISBN: 978-1-4939-8949-2
eBook Packages: Springer Protocols