Abstract
The arteriovenous fistula (AVF) is the most common type of vascular access currently used for hemodialysis, but long-term outcomes remain poor in many patients; understanding the basic mechanisms of venous remodeling within the fistula environment is critical to improve our understanding of AVF maturation. In this chapter, we describe a method to create a murine aortocaval fistula that allows intraluminal drug delivery. This model reliably recapitulates human AVF maturation and therefore is a good consideration to study venous remodeling.
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References
Pisoni RL, Zepel L, Port FK, Robinson BM (2015) Trends in US vascular access use, patient preferences, and related practices: an update from the US DOPPS practice monitor with international comparisons. Am J Kidney Dis 65(6):905–915. https://doi.org/10.1053/j.ajkd.2014.12.014
Gibson KD, Gillen DL, Caps MT, Kohler TR, Sherrard DJ, Stehman-Breen CO (2001) Vascular access survival and incidence of revisions: a comparison of prosthetic grafts, simple autogenous fistulas, and venous transposition fistulas from the United States Renal Data System Dialysis Morbidity and Mortality Study. J Vasc Surg 34(4):694–700. https://doi.org/10.1067/mva.2001.117890
Hu H, Patel S, Hanisch J, Santana J, Hashimoto T, Bai H, Kudze T, Foster T, Guo J, Yatsula B, Tsui J, Dardik A (2016) Future research directions to improve fistula maturation and reduce access failure. Semin Vasc Surg 29(4):153–171. https://doi.org/10.1053/j.semvascsurg.2016.08.005
Yamamoto K, Li X, Shu C, Miyata T, Dardik A (2013) Technical aspects of the mouse aortocaval fistula. J Vis Exp 77:e50449. https://doi.org/10.3791/50449
Lu DY, Chen EY, Wong DJ, Yamamoto K, Protack CD, Williams WT, Assi R, Hall MR, Sadaghianloo N, Dardik A (2014) Vein graft adaptation and fistula maturation in the arterial environment. J Surg Res 188:162–173. https://doi.org/10.1016/j.jss.2014.01.042
Hashimoto T, Yamamoto K, Foster T, Bai H, Shigematsu K, Dardik A (2016) Intraluminal drug delivery to the mouse arteriovenous fistula endothelium. J Vis Exp 109:e53905. https://doi.org/10.3791/53905
Castier Y, Lehoux S, Hu Y, Foteinos G, Tedgui A, Xu Q (2006) Characterization of neointima lesions associated with arteriovenous fistulas in a mouse model. Kidney Int 70(2):315–320. https://doi.org/10.1038/sj.ki.5001569
Yamamoto K, Protack CD, Tsuneki M, Hall MR, Wong DJ, Lu DY, Assi R, Williams WT, Sadaghianloo N, Bai H, Miyata T, Madri JA, Dardik A (2013) The mouse aortocaval fistula recapitulates human arteriovenous fistula maturation. Am J Physiol Heart Circ Physiol 305(12):H1718–H1725. https://doi.org/10.1152/ajpheart.00590.2013
Yamamoto K, Protack CD, Kuwahara G, Tsuneki M, Hashimoto T, Hall MR, Assi R, Brownson KE, Foster TR, Bai H, Wang M, Madri JA, Dardik A (2015) Disturbed shear stress reduces Klf2 expression in arterial-venous fistulae in vivo. Phys Rep 3. https://doi.org/10.14814/phy2.12348
Acknowledgments
This work was supported in part by the National Institutes of Health Grant R01-HL128406 and the Merit Review Award I01-BX002336 from the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Program, as well as through the resources and the use of facilities at the Veterans Affairs Connecticut Healthcare System (West Haven, CT).
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Isaji, T., Dardik, A. (2018). The Mouse Aortocaval Fistula Model with Intraluminal Drug Delivery. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_21
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_21
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