Abstract
The principle of the overall hemostatic potential (OHP) assay is the generation of a fibrin time curve by optical density readings, which represent the balance between fibrin generation, triggered by thrombin or tissue factor, and fibrinolysis, triggered by tissue plasminogen activator (tPA). OHP measures the integrated effect of procoagulant, anticoagulant, and fibrinolytic factors, and OHP assay parameters provide a means of quantifying both the coagulation and fibrinolytic potential of platelet poor plasma. In particular, the OHP assay can be used to evaluate hypercoagulable states and abnormalities of fibrinolysis which are not well defined by assays which are routinely available in the coagulation laboratory. OHP is a technically simple assay, with potential for application in the routine laboratory at minimal cost.
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Acknowledgements
I would like to thank Professor Christopher M. Ward and Dr. Marie-Christine Morel-Kopp for PhD thesis supervision and advice during assay development and optimization at the Northern Blood Research Centre, Sydney NSW, Australia. I also thank Dr. Caroline Reddel for assistance with further assay modification and application in a mouse model, at Vascular Biology Group, ANZAC Research Institute, Sydney NSW, Australia.
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Curnow, J. (2017). The Overall Hemostatic Potential (OHP) Assay. In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_38
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DOI: https://doi.org/10.1007/978-1-4939-7196-1_38
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