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Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

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Hemostasis and Thrombosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1646))

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

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References

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Author information

Authors and Affiliations

  1. Departments of Clinical and Laboratory Haematology, Institute of Clinical Pathology and Medical Research, WestmeadHospital, Westmead, NSW 2145, Australia

    Leonardo Pasalic

  2. Sydney Centres for Thrombosis and Haemostasis, Westmead, NSW, Australia

    Leonardo Pasalic

  3. ANZAC Research Institute, University of Sydney, Sydney, NSW, Australia

    Gabrielle J. Pennings, Leonard Kritharides & Vivien M. Chen

  4. Blood, Stem Cell and Cancer Research Unit, St Vincent’s Centre for Applied Medical Research, Sydney, NSW, Australia

    David Connor

  5. Prince of Wales Clinical School, Faculty of Medicine, University Of New South Wales, Sydney, NSW, Australia

    Heather Campbell & Vivien M. Chen

  6. Department of Cardiology, Concord Repatriation General Hospital, Sydney, NSW, Australia

    Leonard Kritharides

  7. Department of Haematology, Concord Repatriation General Hospital, Sydney, NSW, Australia

    Vivien M. Chen

Authors
  1. Leonardo Pasalic
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  2. Gabrielle J. Pennings
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  3. David Connor
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  4. Heather Campbell
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  5. Leonard Kritharides
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  6. Vivien M. Chen
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Corresponding author

Correspondence to Leonardo Pasalic .

Editor information

Editors and Affiliations

  1. Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, New South Wales, Australia

    Emmanuel J. Favaloro

  2. Section of Clinical Biochemistry, University of Verona, Verona, Italy

    Giuseppe Lippi

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Pasalic, L., Pennings, G.J., Connor, D., Campbell, H., Kritharides, L., Chen, V.M. (2017). Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood. In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_28

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  • DOI: https://doi.org/10.1007/978-1-4939-7196-1_28

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7194-7

  • Online ISBN: 978-1-4939-7196-1

  • eBook Packages: Springer Protocols

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