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Solid Phase Immunoassay for the Detection of Anti-β2 Glycoprotein I Antibodies

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Hemostasis and Thrombosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1646))

Abstract

The discovery in the 1990s that the protein beta-2-glycoprotein I (β2GPI) was an antigen of central importance in the antiphospholipid syndrome (APS) was soon followed by the development of ELISA assays capable of identifying anti-β2GPI antibodies recognizing this antigen. The determination of these antibodies has continued to play a major role in the management of APS patients because of their extremely high specificity for diagnosing the disease, despite their relatively poor sensitivity compared to anticardiolipin antibodies. Additionally, there is abundant evidence of the pathogenic capacity of these antibodies. In this chapter we outline the procedure for producing essential assay components and for performing the anti-β2GPI ELISA, which can be used to determine the presence of IgG, IgM and IgA anti-β2GPI antibodies in human samples. We also provide general guidelines that will facilitate optimal performance of the anti-β2GPI ELISA assay.

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Acknowledgement

RW is supported by a Mallinckrodt-Questcor Fellowship Grant and both RW and EP are supported by an NIH Grant R01 AR056745-01A1.

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Authors and Affiliations

  1. Antiphospholipid Standardization Laboratory, Division of Rheumatology, Department of Internal Medicine, University of Texas Medical Branch, 301 University Blvd., Galveston, TX, 77555-0883, USA

    Rohan Willis & Elizabeth Papalardo

  2. University of the West Indies, Mona Campus, Kingston, Jamaica, WI

    E. Nigel Harris

Authors
  1. Rohan Willis
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  2. Elizabeth Papalardo
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  3. E. Nigel Harris
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Corresponding author

Correspondence to Rohan Willis .

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Editors and Affiliations

  1. Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, New South Wales, Australia

    Emmanuel J. Favaloro

  2. Section of Clinical Biochemistry, University of Verona, Verona, Italy

    Giuseppe Lippi

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Willis, R., Papalardo, E., Nigel Harris, E. (2017). Solid Phase Immunoassay for the Detection of Anti-β2 Glycoprotein I Antibodies. In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7196-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7194-7

  • Online ISBN: 978-1-4939-7196-1

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