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Rolling Circle Transcription for the Self-Assembly of Multimeric RNAi Structures and Its Applications in Nanomedicine

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RNA Nanostructures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1632))

Abstract

The enzymatic process of rolling circle transcription (RCT) enables self-assembly of multimeric RNAi structures from a circular DNA template. The self-assembled RNAi structures can be manipulated easily by simple base pairing rules with short DNA fragments for constructing multifunctional nanoparticles in the field of nanomedicine. Here we describe the method to generate multifunctional RNAi nanoparticles applicable in nanomedicine.

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References

  1. Shu Y, Pi F, Sharma A, Rajabi M, Haque F, Shu D, Leggas M, Evers BM, Guo P (2014) Stable RNA nanoparticles as potential new generation drugs for cancer therapy. Adv Drug Deliv Rev 66:74–89

    Article  CAS  PubMed  Google Scholar 

  2. Burnett JC, Rossi JJ (2012) RNA-based therapeutics: current progress and future prospects. Chem Biol 19:60–71

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Schiffelers RM, Ansari A, Xu J, Zhou Q, Tang Q, Storm G, Molema G, Lu PY, Scaria PV, Woodle MC (2004) Cancer siRNA therapy by tumor selective delivery with ligand-targeted sterically stabilized nanoparticle. Nucleic Acids Res 32:e149

    Article  PubMed  PubMed Central  Google Scholar 

  4. Yin JQ, Gao J, Shao R, Tian WN, Wang J, Wan Y (2003) siRNA agents inhibit oncogene expression and attenuate human tumor cell growth. J Exp Ther Oncol 3:194–204

    Article  CAS  PubMed  Google Scholar 

  5. Jiang M, Milner J (2002) Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene 21:6041–6048

    Article  CAS  PubMed  Google Scholar 

  6. Bobbin ML, Rossi JJ (2016) RNA Interference (RNAi)-Based therapeutics: delivering on the promise? Annu Rev Pharmacol Toxicol 56:103–122

    Article  CAS  PubMed  Google Scholar 

  7. Jang M, Kim JH, Nam HY, Kwon IC, Ahn HJ (2015) Design of a platform technology for systemic delivery of siRNA to tumours using rolling circle transcription. Nat Commun 6:7930

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Lee JB, Hong J, Bonner DK, Poon Z, Hammond PT (2012) Self-assembled RNA interference microsponges for efficient siRNA delivery. Nat Mater 11:316–322

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Daly TA, Wang M, Regen SL (2011) The origin of cholesterol's condensing effect. Langmuir 27:2159–2161

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Ge Q, Ilves H, Dallas A, Kumar P, Shorenstein J, Kazakov SA, Johnston BH (2010) Minimal-length short hairpin RNAs: the relationship of structure and RNAi activity. RNA 16:106–117

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Han D, Park Y, Nam H, Lee JB (2014) Enzymatic size control of RNA particles using complementary rolling circle transcription (cRCT) method for efficient siRNA production. Chem Commun (Camb) 50:11665–11667

    Article  CAS  Google Scholar 

  12. Meyer RR, Simpson MV (1969) DNA biosynthesis in mitochondria. Differential inhibition of mitochondrial and nuclear DNA polymerases by the mutagenic dyes ethidium bromide and acriflavin. Biochem Biophys Res Commun 34:238–244

    Article  CAS  PubMed  Google Scholar 

  13. Kuhn H, Demidov VV, Coull JM, Fiandaca MJ, Gildea BD, Frank-Kamenetskii MD (2002) Hybridization of DNA and PNA molecular beacons to single-stranded and double-stranded DNA targets. J Am Chem Soc 124:1097–1103

    Article  CAS  PubMed  Google Scholar 

  14. Straus NA, Bonner TI (1972) Temperature dependence of RNA-DNA hybridization kinetics. Biochim Biophys Acta 277:87–95

    Article  CAS  PubMed  Google Scholar 

  15. Podder SK (1971) Co-operative non-enzymic base recognition. A kinetic study of interaction between GpGpGpC and GpCpCpC and of self-association of GpGpGpC. Eur J Biochem 22:467–477

    Article  CAS  PubMed  Google Scholar 

  16. Vlashi E, Kelderhouse LE, Sturgis JE, Low PS (2013) Effect of folate-targeted nanoparticle size on their rates of penetration into solid tumors. ACS Nano 7:8573–8582

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul, 136-791, South Korea

    Mihue Jang & Hyung Jun Ahn

Authors
  1. Mihue Jang
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  2. Hyung Jun Ahn
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Corresponding author

Correspondence to Hyung Jun Ahn .

Editor information

Editors and Affiliations

  1. Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Frederick, Maryland, USA

    Eckart Bindewald

  2. RNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA

    Bruce A. Shapiro

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Jang, M., Ahn, H.J. (2017). Rolling Circle Transcription for the Self-Assembly of Multimeric RNAi Structures and Its Applications in Nanomedicine. In: Bindewald, E., Shapiro, B. (eds) RNA Nanostructures . Methods in Molecular Biology, vol 1632. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7138-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7138-1_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7137-4

  • Online ISBN: 978-1-4939-7138-1

  • eBook Packages: Springer Protocols

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