Abstract
Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA–DNA hybrids activating RNAi and blocking HIV replication in human cells.
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Martins, A.N. et al. (2017). Intracellular Reassociation of RNA–DNA Hybrids that Activates RNAi in HIV-Infected Cells. In: Bindewald, E., Shapiro, B. (eds) RNA Nanostructures . Methods in Molecular Biology, vol 1632. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7138-1_18
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DOI: https://doi.org/10.1007/978-1-4939-7138-1_18
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