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Genome Editing of Medaka

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Genome Editing in Animals

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1630))

Abstract

Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.

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References

  1. Takeda H, Shimada A (2010) The art of medaka genetics and genomics: what makes them so unique? Annu Rev Genet 44:217–241. doi:10.1146/annurev-genet-051710-151001

    Article  CAS  PubMed  Google Scholar 

  2. Matsuda M, Nagahama Y, Shinomiya A et al (2002) DMY is a Y-specific DM-domain gene required for male development in the medaka fish. Nature 417:559–563. doi:10.1038/nature751

    Article  CAS  PubMed  Google Scholar 

  3. Omran H, Kobayashi D, Olbrich H et al (2008) Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature 456:611–616. doi:10.1038/nature07471

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ansai S, Kinoshita M (2014) Targeted mutagenesis using CRISPR/Cas system in medaka. Biol Open 3:362–371. doi:10.1242/bio.20148177

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Stemmer M, Thumberger T, Del Sol KM et al (2015) CCTop: an intuitive, flexible and reliable CRISPR/Cas9 target prediction tool. PLoS One 10:e0124633. doi:10.1371/journal.pone.0124633

    Article  PubMed  PubMed Central  Google Scholar 

  6. Moreno-Mateos MA, Vejnar CE, Beaudoin J et al (2015) CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Nat Methods 12:982–988. doi:10.1038/nmeth.3543

  7. Ansai S, Inohaya K, Yoshiura Y et al (2014) Design, evaluation, and screening methods for efficient targeted mutagenesis with transcription activator-like effector nucleases in medaka. Dev Growth Differ 56:98–107. doi:10.1111/dgd.12104

    Article  CAS  PubMed  Google Scholar 

  8. Kinoshita M, Murata K, Naruse K, Tanaka M (2009) Medaka: biology, management, and experimental protocols. Wiley-Blackwell, Ames. doi:10.1002/9780813818849

    Book  Google Scholar 

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Acknowledgments

This work was supported by a Grant-in-Aid for JSPS Research Fellows (Grant Number 13J01682)(SA).

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Authors and Affiliations

  1. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Kyoto, Japan

    Satoshi Ansai & Masato Kinoshita

  2. Division of Ecological Genetics, Department of Population Genetics, National Institute of Genetics, Yata 1111, Mishima, 411-8540, Shizuoka, Japan

    Satoshi Ansai

Authors
  1. Satoshi Ansai
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  2. Masato Kinoshita
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Corresponding author

Correspondence to Masato Kinoshita .

Editor information

Editors and Affiliations

  1. Laboratory of Genome Science Biosignal Genome Resource Center Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan

    Izuho Hatada

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Ansai, S., Kinoshita, M. (2017). Genome Editing of Medaka. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7128-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7127-5

  • Online ISBN: 978-1-4939-7128-2

  • eBook Packages: Springer Protocols

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