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Diagnostic Bacteriology: Raman Spectroscopy

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Diagnostic Bacteriology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1616))

Abstract

Current clinical methodology for identification of bacterial infections relies predominantly on culturing microbes from patient material and performing biochemical tests. This can often be an inefficient and lengthy process, which has a significant detrimental effect upon patient care. Techniques used in other aspects of molecular research have the potential to revolutionize the way in which diagnostic tests are used and delivered in the clinical setting. The need for rapid, accurate, and cost-effective molecular techniques in the diagnostic laboratory is imperative to improving patient care, preventing the spread of drug resistance and decreasing the overall burden associated with nosocomial infections. Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS) are powerful vibrational spectroscopy techniques that are being developed for highly sensitive pathogen identification in complex clinical samples. Raman spectroscopy is a molecular technique that is capable of probing samples noninvasively and nondestructively. It has been used with high specificity to assess tissue and bacterial samples at the molecular level with diverse clinical and diagnostic applications. SERS has recently developed out of the advances in the Raman spectroscopy arena. This technique is designed to amplify Raman scattering and allows for better differentiation of bacterial isolates. Although the current parameters for the use of SERS require a pure culture and are relatively monoparametric, current breakthroughs and testing are pushing the technology to new levels and thus changing the face of modern bacterial diagnostics.

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References

  1. Livermore DM, Wain J (2013) Revolutionising bacteriology to improve treatment outcomes and antibiotic stewardship. Infect Chemother 45:1–10

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Kootallur BN, Thangavelu CP, Mani M (2011) Bacterial identification in the diagnostic laboratory: how much is enough? Indian J Med Microbiol 29:336–340

    Article  CAS  PubMed  Google Scholar 

  3. Wills H et al (2009) Raman spectroscopy detects and distinguishes neuroblastoma and related tissues in fresh and (banked) frozen specimens. J Pediatr Surg 44:386–391

    Article  PubMed  Google Scholar 

  4. Harvey TJ et al (2008) Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers. J Biomed Opt 13:064004

    Article  PubMed  Google Scholar 

  5. Andrade PO et al (2007) Study of normal colorectal tissue by FT-Raman spectroscopy. Anal Bioanal Chem 387:1643–1648

    Article  CAS  PubMed  Google Scholar 

  6. Buschman HP et al (2001) Raman microspectroscopy of human coronary atherosclerosis: biochemical assessment of cellular and extracellular morphologic structures in situ. Cardiovasc Pathol 10:69–82

    Article  CAS  PubMed  Google Scholar 

  7. Carden A, Rajachar RM, Morris MD, Kohn DH (2003) Ultrastructural changes accompanying the mechanical deformation of bone tissue: a Raman imaging study. Calcif Tissue Int 72:166–175

    Article  CAS  PubMed  Google Scholar 

  8. Chan KL et al (2008) A coordinated approach to cutaneous wound healing: vibrational microscopy and molecular biology. J Cell Mol Med 12(5b):2145–2154

    Article  CAS  PubMed Central  Google Scholar 

  9. Chowdary MV et al (2007) Discrimination of normal and malignant mucosal tissues of the colon by Raman spectroscopy. Photomed Laser Surg 25:269–274

    Article  CAS  PubMed  Google Scholar 

  10. Crane NJ, Popescu V, Morris MD, Steenhuis P, Ignelzi MA Jr (2006) Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembraneous mineralization. Bone 39:434–442

    Article  CAS  PubMed  Google Scholar 

  11. Haka AS et al (2006) In vivo margin assessment during partial mastectomy breast surgery using Raman spectroscopy. Cancer Res 66:3317–3322

    Article  CAS  PubMed  Google Scholar 

  12. Jess PR et al (2007) Early detection of cervical neoplasia by Raman spectroscopy. Int J Cancer 121:2723–2728

    Article  CAS  PubMed  Google Scholar 

  13. Koljenovic S et al (2007) Raman spectroscopic characterization of porcine brain tissue using a single fiber-optic probe. Anal Chem 79:557–564

    Article  CAS  PubMed  Google Scholar 

  14. Leroy G, Penel G, Leroy N, Brès E (2002) Human tooth ename: a Raman polarized approach. Appl Spectrosc 56:1030–1034

    Article  CAS  Google Scholar 

  15. McGill N, Dieppe PA, Bowden M, Gardiner DJ, Hall M (1991) Identification of pathological mineral deposits by Raman microscopy. Lancet 337:77–78

    Article  CAS  PubMed  Google Scholar 

  16. Robichaux-Viehoever A et al (2007) Characterization of Raman spectra measured in vivo for the detection of cervical dysplasia. Appl Spectrosc 61:986–993

    Article  CAS  PubMed  Google Scholar 

  17. Shetty G, Kendall C, Shepherd N, Stone N, Barr H (2006) Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagus. Br J Cancer 94:1460–1464

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Shim MG, Wilson BC, Marple E, Wach M (1999) Study of fiber-optic probes for in vivo medical Raman spectroscopy. Appl Spectrosc 53:619–627

    Article  CAS  Google Scholar 

  19. Wang TD, Van Dam J (2004) Optical biopsy: a new frontier in endoscopic detection and diagnosis. Clin Gastroenterol Hepatol 2:744–753

    Article  PubMed  PubMed Central  Google Scholar 

  20. LaPlant F (2010) In: Matousek P, Morris MD (eds) Emerging Raman applications and techniques in biomedical and pharmaceutical fields. Springer-Verlag, Berlin, pp 3–4

    Google Scholar 

  21. Le Ru EC, Etchegoin PG (2009) Principles of surface-enhanced Raman spectroscopy and related plasmonic effects. Elsevier, Oxford

    Google Scholar 

  22. Fleischmann M, Hendra PJ, McQuillan AJ (1974) Raman spectra of pyridine adsorbed at a silver electrode. Chem Phys Lett 26(2):163–166

    Article  CAS  Google Scholar 

  23. Buijtels PC et al (2008) Rapid identification of mycobacteria by Raman spectroscopy. J Clin Microbiol 46:961–965

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Escoriza MF, VanBriesen JM, Stewart S, Maier J, Treado PJ (2006) Raman spectroscopy and chemical imaging for quantification of filtered waterborne bacteria. J Microbiol Methods 66:63–72

    Article  CAS  PubMed  Google Scholar 

  25. Maquelin K et al (2002) Identification of medically relevant microorganisms by vibrational spectroscopy. J Microbiol Methods 51:255–271

    Article  CAS  PubMed  Google Scholar 

  26. Wu Q et al (2000) Intensities of E. coli nucleic acid Raman spectra excited selectively from whole cells with 251-nm light. Anal Chem 72:2981–2986

    Article  CAS  PubMed  Google Scholar 

  27. Zeiri L, Bronk BV, Shabtai Y, Eichler J, Efrima S (2004) Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria. Appl Spectrosc 58:33–40

    Article  CAS  PubMed  Google Scholar 

  28. Maquelin K, Dijkshoorn L, van der Reijden TJ, Puppels GJ (2006) Rapid epidemiological analysis of Acinetobacter strains by Raman spectroscopy. J Microbiol Methods 64:126–131

    Article  CAS  PubMed  Google Scholar 

  29. Maquelin K et al (2000) Raman spectroscopic method for identification of clinically relevant microorganisms growing on solid culture medium. Anal Chem 72:12–19

    Article  CAS  PubMed  Google Scholar 

  30. Kalasinsky KS et al (2007) Raman chemical imaging spectroscopy reagentless detection and identification of pathogens: signature development and evaluation. Anal Chem 79:2658–2673

    Article  CAS  PubMed  Google Scholar 

  31. Zeiri L, Bronk BV, Shabtai Y, Czege J, Efrima S (2002) Silver metal induced surface enhanced Raman of bacteria. Colloids Surf A Physicochem Eng Asp 208:357–362

    Article  CAS  Google Scholar 

  32. Ghebremedhin M, Yesupriya S, Luka J, Crane NJ (2015) Validation of hierarchical cluster analysis for identification of bacterial species using 42 bacterial isolates. Proc SPIE 9318:93180W

    Article  Google Scholar 

  33. Premasiri WR, Sauer-Budge AF, Lee JC, Klapperich CM, Ziegler LD (2012) Rapid bacterial diagnostics via surface enhanced Raman microscopy. Spectroscopy (Springf) 27:s8–s31

    CAS  Google Scholar 

  34. Ozaki Y, Kneipp K, Aroca R (2014) Frontiers of surface-enhanced Raman scattering: single nanoparticles and single cells. Wiley, West Sussex

    Google Scholar 

  35. Rosch P et al (2005) Chemotaxonomic identification of single bacteria by micro-Raman spectroscopy: application to clean-room-relevant biological contaminations. Appl Environ Microbiol 71:1626–1637

    Article  PubMed  PubMed Central  Google Scholar 

  36. Premasiri WR, Moir DT, Klempner MS, Zeigler LD (2007) In: Kneipp K, Aroca R, Kneupp H, Wentrup-Byrne E (eds) New approaches in biochemical spectroscopy. Oxford University Press, New York, p 164

    Google Scholar 

  37. Crane NJ, Elster EA (2012) Profiling wound healing with wound effluent: Raman spectroscopic indicators of infection. Proc SPIE 8220:82200S

    Article  Google Scholar 

  38. National Academies of Sciences, Engineering, and Medicine (2015) Improving diagnosis in health care. The National Academies Press, Washington, DC

    Google Scholar 

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Acknowledgments

This work was prepared as part of the authors’ official duties. Title 17 U.S.C. §105 provides that “Copyright protection under this title is not available for any work of the United States Government.” Title 17 U.S.C. §101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties. The views expressed in this article are those of the author and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. This effort was supported (in part) by the U.S. Navy Bureau of Medicine and Surgery under the Medical Development Program and Office of Naval Research work unit number (602115HP.3720.001.A1015) and USAMRAA award W81XWH-13-2-0039. This study was performed under MUA 228 with the Walter Reed Army Institute of Research. I/We certify that all individuals who qualify as authors have been listed; each has participated in the conception and design of this work, the analysis of data (when applicable), the writing of the document, and the approval of the submission of this version; that the document represents valid work; that if we used information derived from another source, we obtained all necessary approvals to use it and made appropriate acknowledgements in the document; and that each takes public responsibility for it.

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Authors and Affiliations

  1. Naval Medical Research Center-Asia, Singapore, Singapore

    Rebecca L. Pavlicek

  2. The Department of Surgery at Uniformed Services University of the Health Sciences & The Walter Reed National Military Medical Center, Bethesda, MD, USA

    Nicole J. Crane & Eric A. Elster M.D.

  3. Department of Regenerative Medicine, Naval Medical Research Center, Silver Spring, MD, USA

    Meron Ghebremedhin & Katherine E. Cilwa

Authors
  1. Rebecca L. Pavlicek
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  2. Nicole J. Crane
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  3. Meron Ghebremedhin
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  4. Katherine E. Cilwa
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  5. Eric A. Elster M.D.
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Corresponding author

Correspondence to Eric A. Elster M.D. .

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Editors and Affiliations

  1. Germantown, Maryland, USA

    Kimberly A. Bishop-Lilly

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Pavlicek, R.L., Crane, N.J., Ghebremedhin, M., Cilwa, K.E., Elster, E.A. (2017). Diagnostic Bacteriology: Raman Spectroscopy. In: Bishop-Lilly, K. (eds) Diagnostic Bacteriology. Methods in Molecular Biology, vol 1616. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7037-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7037-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7035-3

  • Online ISBN: 978-1-4939-7037-7

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