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Reverse Genetics Systems for Filoviruses

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Reverse Genetics of RNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1602))

Abstract

Filoviruses are among the most pathogenic viruses known to man. Reverse genetics systems, in particular full-length clone systems, allow the generation of recombinant filoviruses, which can be used to study virus biology, but also for applied uses such as screening for countermeasures. Here we describe the generation of recombinant filoviruses from cDNA.

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Acknowledgments

The authors thank Allison Groseth for critical reading of the manuscript. This work was supported by the Intramural Research Program of the National Institutes of Health, NIAID.

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Authors and Affiliations

  1. Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Thomas Hoenen & Heinz Feldmann

  2. Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany

    Thomas Hoenen

Authors
  1. Thomas Hoenen
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  2. Heinz Feldmann
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Corresponding author

Correspondence to Thomas Hoenen .

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Editors and Affiliations

  1. Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA

    Daniel R. Perez

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Hoenen, T., Feldmann, H. (2017). Reverse Genetics Systems for Filoviruses. In: Perez, D. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 1602. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6964-7_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6964-7_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6962-3

  • Online ISBN: 978-1-4939-6964-7

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