Abstract
Hydrogen peroxide (H2O2) plays important roles in many physiological and pathological processes. At the cellular organelle level, the abnormal concentrations of H2O2 in the lysosomes may cause redox imbalance and the loss of the critical functions of the lysosomes. Herein, we describe the preparation of a potent lysosome-targeted two-photon fluorescent probe (Lyso-HP) for the detection of H2O2 in the lysosomes in the living cells. This unique fluorescent probe can also be employed to effectively detect H2O2 in the living tissues using two-photon fluorescence microscopy.
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Acknowledgment
This work was financially supported by NSFC (21472067, 21502067, 21672083), the Natural Science Foundation of Shandong Province, China (ZR2014BP001), Taishan Scholar Foundation (TS 201511041), and the startup fund of University of Jinan (309-10004, 160082101).
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Ren, M., Deng, B., Kong, X., Tang, Y., Lin, W. (2017). Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues. In: Öllinger, K., Appelqvist, H. (eds) Lysosomes. Methods in Molecular Biology, vol 1594. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6934-0_7
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DOI: https://doi.org/10.1007/978-1-4939-6934-0_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6932-6
Online ISBN: 978-1-4939-6934-0
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