Abstract
Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e.g., for tissue engineering and regenerative medicine. The methodology described here in detail for SSCs isolated from mouse subcutaneous adipose tissue can be applied to human tissues, such as epicardium.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
D’souza N, Rossignoli F, Golinelli G, Grisendi G, Spano C, Candini O, Osturu S, Catani F, Paolucci P, Horwitz EM, Dominici M (2015) Mesenchymal stem/stromal cells as a delivery platform in cell and gene therapies. BMC Med 13:186
Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228
Lindroos B, Suuronen R, Miettinen S (2011) The potential of adipose stem cells in regenerative medicine. Stem Cell Rev 7:269–291
Gimble JM, Bunnell BA, Chiu ES, Guilak F (2011) Concise review: adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation. Stem Cells 29:749–754
Toyoda M, Matsubara Y, Lin K, Sugimachi K, Furue M (2009) Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues. Cell Biochem Funct 27:440–447
Di Taranto G, Cicione C, Visconti G, Isgrò MA, Barba M, Di Stasio E, Stigliano E, Bernardini C, Michetti F, Salgarello M, Lattanzi W (2015) Qualitative and quantitative differences of adipose-derived stromal cells from superficial and deep subcutaneous lipoaspirates: a matter of fat. Cytotherapy 17:1076–1089
Clunie-O’Connor C, Smits AM, Antoniades C, Russell AJ, Yellon DM, Goumans M-J, Riley PR (2015) The derivation of primary human epicardium-derived cells. Curr Protoc Stem Cell Biol 35:2C.5.1–2C.5.12
Qureshi AT, Chen C, Shah F, Thomas-Porch C, Gimble JM, Hayes DJ (2014) Human adipose-derived stromal/stem cell isolation, culture, and osteogenic differentiation. Methods Enzymol 538:67–88
Riis S, Zachar V, Boucher S, Vemuri MC, Pennisi CP, Fink T (2015) Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy. Expert Rev Mol Med 17:e11
Devitt SM, Carter CM, Dierov R, Weiss S, Gersch RP, Percec I (2015) Successful isolation of viable adipose-derived stem cells from human adipose tissue subject to long-term cryopreservation: positive implications for adult stem cell-based therapeutics in patients of advanced age. Stem Cells Int 2015:146421
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Prat, M., Oltolina, F., Antonini, S., Zamperone, A. (2017). Isolation of Stromal Stem Cells from Adipose Tissue. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6756-8_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6754-4
Online ISBN: 978-1-4939-6756-8
eBook Packages: Springer Protocols