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Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria

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Plant Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1424))

Abstract

NO is a free radical with short half-life and high reactivity; due to its physiochemical properties it is very difficult to detect the concentrations precisely. Chemiluminescence is one of the robust methods to quantify NO. Detection of NO by this method is based on reaction of nitric oxide with ozone which leads to emission of light and amount of light is proportional to NO. By this method NO can be measured in the range of pico moles to nano moles range. Using direct chemiluminescence method, NO emitted into the gas stream can be detected whereas using indirect chemiluminescence oxidized forms of NO can also be detected. We detected NO using purified nitrate reductase, mitochondria, cell suspensions, and roots; detail measurement method is described here.

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Acknowledgments

This work was supported by Ramalingaswami Fellowship funded to JGK by DBT. I thank Werner Kaiser, University of Wuerzburg, for introducing chemiluminescence method. RJ and PKP are currently funded by UGC Fellowship for doctoral studies.

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    Authors and Affiliations

    1. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, 10531, New Delhi, 10067, India

      Aakanksha Wany, Alok Kumar Gupta, Aprajita Kumari, Shika Gupta, Sonal Mishra, Ritika Jaintu, Pradeep K. Pathak & Kapuganti Jagadis Gupta

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    1. Aakanksha Wany
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    2. Alok Kumar Gupta
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    Correspondence to Kapuganti Jagadis Gupta .

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    1. National Institute of Plant Genome Resea, New Delhi, India

      Kapuganti Jagadis Gupta

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    Wany, A. et al. (2016). Chemiluminescence Detection of Nitric Oxide from Roots, Leaves, and Root Mitochondria. In: Gupta, K. (eds) Plant Nitric Oxide. Methods in Molecular Biology, vol 1424. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3600-7_2

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    • DOI: https://doi.org/10.1007/978-1-4939-3600-7_2

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3598-7

    • Online ISBN: 978-1-4939-3600-7

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