Skip to main content
SpringerLink
Log in

Laser-Based Methods for Detection of Nitric Oxide in Plants

  • Protocol
  • First Online:
Plant Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1424))

Abstract

Nitric oxide (NO) plays an important role in plant signaling and in response to various stress conditions. Therefore, real-time measurements of NO production provide better insights into understanding plant processes and can help developing strategies to improve food production and postharvest quality. Using laser-based spectroscopic methods, sensitive, online, in planta measurements of plant-pathogen interactions are possible. This chapter introduces the basic principle of the optical detectors using different laser sources for accurate monitoring of fast dynamic changes of NO production. Several applications are also presented to demonstrate the suitability of these detectors for detection of NO in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wink DA, Grisham MB, Mitchell JB et al (1996) Direct and indirect effects of nitric oxide in chemical reactions relevant to biology. Methods Enzymol 268:12–31

    Article  CAS  PubMed  Google Scholar 

  2. Gow AJ, Ischiropoulos H (2001) Nitric oxide chemistry and cellular signaling. J Cell Physiol 187(3):277–282

    Article  CAS  PubMed  Google Scholar 

  3. Domingos P, Prado AM, Wong A et al (2015) Nitric oxide: a multitasked signaling gas in plants. Mol Plant 8(4):506–520. doi:10.1016/j.molp.2014.12.010

    Article  CAS  PubMed  Google Scholar 

  4. Mur LAJ, Mandon J, Persijn S et al (2013) Nitric oxide in plants: an assessment of the current state of knowledge. AoB Plants. doi:10.1093/aobpla/pls052

    PubMed  PubMed Central  Google Scholar 

  5. Scheler C, Durner J, Astier J (2013) Nitric oxide and reactive oxygen species in plant biotic interactions. Curr Opin Plant Biol 16(4):534–539

    Article  CAS  PubMed  Google Scholar 

  6. Trapet P, Kulik A, Lamotte O et al (2015) NO signaling in plant immunity: a tale of messengers. Phytochemistry 112:72–79

    Article  CAS  PubMed  Google Scholar 

  7. Hufton CA, Besford RT, Wellburn AR (1996) Effects of NO (+NO2) pollution on growth, nitrate reductase activities and associated protein contents in glasshouse lettuce grown hydroponically in winter with CO2 enrichment. New Phytol 133(3):495–501

    Article  CAS  Google Scholar 

  8. Beligni MV, Lamattina L (2000) Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light-inducible responses in plants. Planta 210(2):215–221

    Article  CAS  PubMed  Google Scholar 

  9. Leshem YY, Wills RBH (1998) Harnessing senescence delaying gases nitric oxide and nitrous oxide: a novel approach to postharvest control of fresh horticultural produce. Biol Plant 41(1):1–10

    Article  CAS  Google Scholar 

  10. Mur LAJ, Mandon J, Cristescu SM et al (2011) Methods of nitric oxide detection in plants: a commentary. Plant Sci 181(5):509–519

    Article  CAS  PubMed  Google Scholar 

  11. Patel CKN (1966) Vibrational-rotational laser action in carbon monoxide. Phys Rev 141(1):71–83

    Article  CAS  Google Scholar 

  12. Osgood RM, Eppers WC (1968) High power Co-N2-He laser. Appl Phys Lett 13(12):409–411

    Article  CAS  Google Scholar 

  13. Harris SE (1969) Tunable optical parametric oscillators. Proc IEEE 57(12):2096–2113

    Article  Google Scholar 

  14. Giordmai JA, Miller RC (1965) Tunable coherent parametric oscillation in Linbo3 at optical frequencies. Phys Rev Lett 14(24):973–976

    Article  Google Scholar 

  15. Baxter GW, Payne MA, Austin BDW et al (2000) Spectroscopic diagnostics of chemical processes: applications of tunable optical parametric oscillators. Appl Phys B 71(5):651–663

    Article  CAS  Google Scholar 

  16. Partin DL (1985) Lead salt quantum well diode-lasers. Superlattice Microst 1(2):131–135

    Article  CAS  Google Scholar 

  17. Tacke M (1995) New developments and application of tunable Ir lead salt lasers. Infrared Phys Technol 36(1):447–463

    Article  CAS  Google Scholar 

  18. Clemitshaw KC (2004) A review of instrumentation and measurement techniques for ground-based and airborne field studies of gas-phase tropospheric chemistry. Crit Rev Env Sci Technol 34(1):1–108

    Article  CAS  Google Scholar 

  19. Parrish DD, Fehsenfeld FC (2000) Methods for gas-phase measurements of ozone, ozone precursors and aerosol precursors. Atmos Environ 34(12-14):1921–1957

    Article  CAS  Google Scholar 

  20. Kondo Y, Kawakami S, Koike M et al (1997) Performance of an aircraft instrument for the measurement of NOy. J Geophys Res Atmos 102(D23):28663–28671

    Article  CAS  Google Scholar 

  21. Rockel P, Strube F, Rockel A et al (2002) Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro. J Exp Bot 53(366):103–110

    Article  CAS  PubMed  Google Scholar 

  22. Planchet E, Kaiser WM (2006) Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources. J Exp Bot 57(12):3043–3055

    Article  CAS  PubMed  Google Scholar 

  23. Cristescu SM, Persijn ST, Hekkert STL et al (2008) Laser-based systems for trace gas detection in life sciences. Appl Phys B 92(3):343–349

    Article  CAS  Google Scholar 

  24. Gustafsson LE, Leone AM, Persson MG et al (1991) Endogenous nitric-oxide is present in the exhaled air of rabbits, guinea-pigs and humans. Biochem Biophys Res Commun 181(2):852–857

    Article  CAS  PubMed  Google Scholar 

  25. Kharitonov SA, Yates D, Robbins RA et al (1994) Increased nitric-oxide in exhaled air of asthmatic-patients. Lancet 343(8890):133–135

    Article  CAS  PubMed  Google Scholar 

  26. Buchvald F, Baraidi E, Carraro S et al (2005) Measurements of exhaled nitric oxide in healthy subjects age 4 to 17 years. J Allergy Clin Immun 115(6):1130–1136

    Article  CAS  PubMed  Google Scholar 

  27. Ludviksdottir D, Janson C, Hogman M et al (1999) Exhaled nitric oxide and its relationship to airway responsiveness and atopy in asthma. Respir Med 93(8):552–556

    Article  CAS  PubMed  Google Scholar 

  28. Faist J, Capasso F, Sivco DL et al (1994) Quantum cascade laser. Science 264(5158):553–556

    Article  CAS  PubMed  Google Scholar 

  29. Bernegger S, Sigrist MW (1990) Co-laser photoacoustic spectroscopy of gases and vapours for trace gas analysis. Infrared Phys 30(5):375–429

    Article  Google Scholar 

  30. Mur LAJ, Santosa IE, Laarhoven LJJ et al (2005) Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent Pseudomonas syringae pathovars. Plant Physiol 138(3):1247–1258

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Bijnen FGC, Harren FJM, Hackstein JHP et al (1996) Intracavity CO laser photoacoustic trace gas detection: cyclic CH4, H2O and CO2 emission by cockroaches and scarab beetles. Appl Opt 35(27):5357–5368

    Article  CAS  PubMed  Google Scholar 

  32. Zuckermann H, Harren FJM, Reuss J et al (1997) Dynamics of acetaldehyde production during anoxia and post-anoxia in red bell pepper studied by photoacoustic techniques. Plant Physiol 113(3):925–932

    CAS  PubMed  PubMed Central  Google Scholar 

  33. Martis AAE, Buscher S, Kuhnemann F et al (1998) Simultaneous ethane and ethylene detection using a co-overtone laser photoacoustic spectrometer: a new tool for stress/damage studies in plant physiology. Instrum Sci Technol 26(2-3):177–187

    Article  CAS  Google Scholar 

  34. Dahnke H, Kahl J, Schuler G et al (2000) On-line monitoring of biogenic isoprene emissions using photoacoustic spectroscopy. Appl Phys B 70(2):275–280

    Article  CAS  Google Scholar 

  35. Persijn ST, Veltman RH, Oomens J et al (2000) CO laser absorption coefficients for gases of biological relevance: H2O, CO2, ethanol, acetaldehyde, and ethylene. Appl Spectrosc 54(1):62–71

    Article  CAS  Google Scholar 

  36. Wang Y, Nikodem M, Zhang E et al (2015) Shot-noise limited Faraday rotation spectroscopy for detection of nitric oxide isotopes in breath, urine, and blood. Sci Rep 5:9096

    Article  PubMed  PubMed Central  Google Scholar 

  37. Menzel L, Kosterev AA, Curl RF et al (2001) Spectroscopic detection of biological NO with a quantum cascade laser. Appl Phys B 72(7):859–863

    Article  CAS  PubMed  Google Scholar 

  38. Nelson DD, Shorter JH, McManus JB et al (2002) Sub-part-per-billion detection of nitric oxide in air using a thermoelectrically cooled mid-infrared quantum cascade laser spectrometer. Appl Phys B 75(2–3):343–350

    Article  CAS  Google Scholar 

  39. Moeskops BWM, Cristescu SM, Harren FJM (2006) Sub-part-per-billion monitoring of nitric oxide by use of wavelength modulation spectroscopy in combination with a thermoelectrically cooled, continuous-wave quantum cascade laser. Opt Lett 31(6):823–825

    Article  CAS  PubMed  Google Scholar 

  40. McManus JB, Shorter JH, Nelson DD et al (2008) Pulsed quantum cascade laser instrument with compact design for rapid, high sensitivity measurements of trace gases in air. Appl Phys B 92(3):387–392

    Article  CAS  Google Scholar 

  41. Kosterev AA, Malinovsky AL, Tittel FK et al (2001) Cavity ringdown spectroscopic detection of nitric oxide with a continuous-wave quantum-cascade laser. Appl Opt 40(30):5522–5529

    Article  CAS  PubMed  Google Scholar 

  42. Bakhirkin YA, Kosterev AA, Roller C et al (2004) Mid-infrared quantum cascade laser based off-axis integrated cavity output spectroscopy for biogenic nitric oxide detection. Appl Opt 43(11):2257–2266

    Article  CAS  PubMed  Google Scholar 

  43. Silva ML, Sonnenfroh DM, Rosen DI et al (2005) Integrated cavity output spectroscopy measurements of NO levels in breath with a pulsed room-temperature QCL. Appl Phys B 81(5):705–710

    Article  CAS  Google Scholar 

  44. McCurdy MR, Bakhirkin Y, Wysocki G et al (2007) Performance of an exhaled nitric oxide and carbon dioxide sensor using quantum cascade laser-based integrated cavity output spectroscopy. J Biomed Opt 12(3):034034

    Article  PubMed  Google Scholar 

  45. Ganser H, Urban W, Brown AM (2003) The sensitive detection of NO by Faraday modulation spectroscopy with a quantum cascade laser. Mol Phys 101(4–5):545–550

    Article  CAS  Google Scholar 

  46. Lewicki R, Doty JH, Curl RF et al (2009) Ultrasensitive detection of nitric oxide at 5.33 mu m by using external cavity quantum cascade laser-based Faraday rotation spectroscopy. Proc Natl Acad Sci U S A 106(31):12587–12592

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Kluczynski P, Lundqvist S, Westberg J et al (2011) Faraday rotation spectrometer with sub-second response time for detection of nitric oxide using a cw DFB quantum cascade laser at 5.33 mu m. Appl Phys B 103(2):451–459

    Article  CAS  Google Scholar 

  48. Ganser H, Horstjann M, Suschek CV et al (2004) Online monitoring of biogenic nitric oxide with a QC laser-based Faraday modulation technique. Appl Phys B 78(3–4):513–517

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular and Laser Physics, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands

    Julien Mandon, Frans J. M. Harren & Simona M. Cristescu

  2. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Penglais Campus, Edward Llywd Building, Aberystwyth, Wales, SY23 3DA, UK

    Luis A. J. Mur

Authors
  1. Julien Mandon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luis A. J. Mur
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Frans J. M. Harren
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Simona M. Cristescu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Simona M. Cristescu .

Editor information

Editors and Affiliations

  1. National Institute of Plant Genome Resea, New Delhi, India

    Kapuganti Jagadis Gupta

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Mandon, J., Mur, L.A.J., Harren, F.J.M., Cristescu, S.M. (2016). Laser-Based Methods for Detection of Nitric Oxide in Plants. In: Gupta, K. (eds) Plant Nitric Oxide. Methods in Molecular Biology, vol 1424. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3600-7_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3600-7_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3598-7

  • Online ISBN: 978-1-4939-3600-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation