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Animal Coronaviruses

  • Book
  • © 2016

Overview

Editors:
  1. Leyi Wang

    1. Animal Disease Diagnostic Lab, Ohio Department of Agriculture, Reynoldsburg, USA

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  • Includes cutting-edge methods and protocols for the study of coronaviruses in domestic animals and poultry
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Animal Coronaviruses: A Brief Introduction

      • Leyi Wang, Yan Zhang
      Pages 3-11

  3. Conventional Technologies

    1. Front Matter

      Pages 13-13
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    2. Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues

      • Kwonil Jung
      Pages 15-24

    3. Virus Neutralization Assay for Turkey Coronavirus Infection

      • Yi-Ning Chen, Ching Ching Wu, Tsang Long Lin
      Pages 25-32

    4. Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli

      • Chien Chang Loa, Ching Ching Wu, Tsang Long Lin
      Pages 33-42

    5. Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen

      • Chien Chang Loa, Mohamed Abdelwahab, Yi-Ning Chen, Ming-Kun Hsieh, Ching Ching Wu, Tsang Long Lin
      Pages 43-50

  4. Reverse Genetics

    1. Front Matter

      Pages 51-51
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    2. Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus

      • Sarah M. Keep, Erica Bickerton, Paul Britton
      Pages 53-72

    3. Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses

      • Gergely Tekes
      Pages 73-90

  5. Molecular Diagnostics

    1. Front Matter

      Pages 91-91
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    2. Real-Time RT-PCR Detection of Equine Coronavirus

      • Fabien Miszczak, Nathalie Kin, Vincent Tesson, Astrid Vabret
      Pages 93-100

    3. Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction

      • Amauri Alcindo Alfieri, Alice Fernandes Alfieri, Elisabete Takiuchi
      Pages 101-113

    4. Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus

      • Ramesh Vemulapalli
      Pages 115-119

    5. An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes

      • Junfeng Sun, Shengwang Liu
      Pages 121-130

    6. A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus

      • Chien Chang Loa, Ching Ching Wu, Tsang Long Lin
      Pages 131-138

    7. Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

      • Yi-Ning Chen, Ching Ching Wu, Tsang Long Lin
      Pages 139-150

    8. PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene

      • Yi-Ning Chen, Aydemir Akin, Chien Chang Loa, Mustafa Ababneh, Jianzhong Cao, Wan-Jung Chen et al.
      Pages 151-160

    9. Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis

      • Takehisa Soma
      Pages 161-170
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Keywords

About this book

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

Reviews

“This book is unique in presenting current technologies available for detection of animal coronaviruses and research especially for diagnostic methodologies. The intention is to provide specific protocols and methods for individual coronaviruses, both conventional approaches and state-of-the art technologies. … The primary audience is diagnosticians, interns, and residents who work in the laboratory, but graduate students and researchers will benefit as well. … The book is essential for researchers and diagnosticians in veterinary virology.” (Dongwan Yoo, Doody's Book Reviews, June, 2016)

Editors and Affiliations

  • Animal Disease Diagnostic Lab, Ohio Department of Agriculture, Reynoldsburg, USA

    Leyi Wang

Bibliographic Information

  • Book Title: Animal Coronaviruses

  • Editors: Leyi Wang

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-4939-3414-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3412-6Published: 23 March 2016

  • Softcover ISBN: 978-1-4939-8044-4Published: 19 April 2018

  • eBook ISBN: 978-1-4939-3414-0Published: 22 March 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 218

  • Number of Illustrations: 10 b/w illustrations, 15 illustrations in colour

  • Topics: Infectious Diseases

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

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