Abstract
Screening of peptides to find the ligands that bind to specific targets is an important step in drug discovery. These high-throughput screens require large number of structural variants of peptides to be synthesized and tested. This chapter describes the generation of arrays of peptides on Teflon-patterned sheets of paper. First, the protocol describes the patterning of paper with a Teflon solution to produce arrays with solvophobic barriers that are able to confine organic solvents. Next, we describe the parallel syntheses of 96 peptides on Teflon-patterned arrays using the SPOT synthesis method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lam KS et al (1991) A new type of synthetic peptide library for identifying ligand-binding activity. Nature 354(6348):82–84
Kehoe JW, Kay BK (2005) Filamentous phage display in the new millennium. Chem Rev 105(11):4056–4072
Koivunen E, Wang B, Ruoslahti E (1995) Phage libraries displaying cyclic peptides with different ring sizes: ligand specificities of the RGD-directed integrins. Nat Biotechnol 13(3):265–270
Boder ET, Wittrup KD (1997) Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol 15(6):553–557
Roberts RW, Szostak JW (1997) RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc Natl Acad Sci U S A 94(23):12297–12302
Pauloehrl T et al (2013) Spatially controlled surface immobilization of nonmodified peptides. Angew Chem-Int Ed 52(37):9714–9718
Pirrung MC (1997) Spatially addressable combinatorial libraries. Chem Rev 97(2):473–488
Frank R (1992) Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48(42):9217–9232
Blackwell HE (2006) Hitting the SPOT: small-molecule macroarrays advance combinatorial synthesis. Curr Opin Chem Biol 10(3):203–212
Deiss F et al (2014) Flow-through synthesis on teflon-patterned paper to produce peptide arrays for cell-based assays. Angew Chem-Int Ed 53(25):6374–6377
Hilpert K, Winkler DFH, Hancock REW (2007) Peptide arrays on cellulose support: SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion. Nat Protoc 2(6):1333–1349
Martinez AW et al (2007) Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew Chem Int Ed 46(8):1318–1320
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Deiss, F., Yang, Y., Derda, R. (2016). Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays). In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3136-1_18
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3135-4
Online ISBN: 978-1-4939-3136-1
eBook Packages: Springer Protocols