Abstract
Here, we describe detection of single nucleotide polymorphism (SNP) in genomic DNA samples using a NanoBioArray (NBA) chip. Fast DNA hybridization is achieved in the chip when target DNAs are introduced to the surface-arrayed probes using centrifugal force. Gold nanoparticles (AuNPs) are used to assist SNP detection at room temperature. The parallel setting of sample introduction in the spiral channels of the NBA chip enables multiple analyses on many samples, resulting in a technique appropriate for high-throughput SNP detection. The experimental procedure, including chip fabrication, probe array printing, DNA amplification, hybridization, signal detection, and data analysis, is described in detail.
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© 2016 Springer Science+Business Media New York
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Sedighi, A., Li, P.C.H. (2016). High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device. In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_10
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DOI: https://doi.org/10.1007/978-1-4939-3136-1_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3135-4
Online ISBN: 978-1-4939-3136-1
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