Abstract
Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(l-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(l-lactic acid) microparticles for cartilage tissue engineering.
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References
Decher G, Hong JD (1991) Buildup of ultrathin multilayer films by a self-assembly process, 1 consecutive adsorption of anionic and cationic bipolar amphiphiles on charged surfaces. Makromol Chem Macromol Symp 46:321–327
Facca S, Cortez C, Mendoza-Palomares C et al (2010) Active multilayered capsules for in vivo bone formation. PNAS 107:3406–3411
Mendes AC, Baran ET, Pereira RC et al (2012) Encapsulation and survival of a chondrocyte cell line within xanthan gum derivative. Macromol Biosci 12:350–359
Ying Ma Y, Zhang Y, Liu Y (2013) Investigation of alginate-ε-poly-L-lysine microcapsules for cell microencapsulation. J Biomed Mater Res A 101:1265–1273
Tan W-H, Takeuchi S (2007) Monodisperse alginate hydrogel microbeads for cell encapsulation. Adv Mater 19:2696–2701
Kim J, Arifin DR, Muja N (2011) Multifunctional capsule-in-capsules for immunoprotection and trimodal imaging. Angew Chem 123:2365–2369
Hillberg AL, Kathirgamanathan K, Lam JBB (2013) Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking. J Biomed Mater Res B Appl Biomater 101B:258–268
Costa RR, Mano JF (2014) Polyelectrolyte multilayered assemblies in biomedical technologies. Chem Soc Rev. doi:10.1039/c3cs60393h
Costa RR, Castro E, Arias FJ et al (2013) Multifunctional compartmentalized capsules with a hierarchical organization from the nano to the macro scales. Biomacromolecules 14:2403–2410
Costa RR, Custódio CA, Arias FJ et al (2013) Nanostructured and thermoresponsive recombinant biopolymer-based microcapsules for the delivery of active molecules. Nanomedicine 9:895–902
Caruso F, Caruso RA, Mohwald H (1998) Nanoengineering of inorganic and hybrid hollow spheres by colloidal templating. Science 282:1111–1114
Donath E, Sukhorukov GB, Caruso F et al (1998) Novel hollow polymer shells by colloid-templated assembly of polyelectrolytes. Angew Chem Int Ed 37:2201–2205
Costa NL, Sher P, Mano JF (2011) Liquefied capsules coated with multilayered polyelectrolyte films for cell immobilization. Adv Eng Mater 13:B218–B224
Shchukin D, Sukhorukov G, Mohwald H (2003) Smart inorganic/organic nanocomposite hollow microcapsules. Angew Chem Int Ed 42:4472–4475
Hernández RM, Orive G, Murua A et al (2010) Microcapsules and microcarriers for in situ cell delivery. Adv Drug Deliv Rev 62:711–730
Brun-Graeppi AKAS, Richard C, Bessodes M et al (2011) Cell microcarriers and microcapsules of stimuli-responsive polymers. J Control Release 149:209–224
Correia CR, Sher P, Reis RL et al (2013) Liquified chitosan–alginate multilayer capsules incorporating poly(L-lactic acid) microparticles as cell carriers. Soft Matter 9:2125–2130
Correia CR, Reis RL, Mano JF (2013) Multilayered hierarchical capsules providing cell adhesion sites. Biomacromolecules 14:743–751
Mahmoudifar N, Doran PM (2012) Chondrogenesis and cartilage tissue engineering: the longer road to technology development. Trends Biotechnol 30:166–176
Madry H, Rey-Rico A, Venkatesan JK et al (2013) Transforming growth factor beta-releasing scaffolds for cartilage tissue engineering. Tissue Eng B Rev. doi:10.1089/ten.teb.2013.0271
Jones ME, Messersmith PB (2007) Facile coupling of synthetic peptides and peptide-polymer conjugates to cartilage via transglutaminase enzyme. Biomaterials 28:5215–5224
Niger C, Beazley KE, Nurminskaya M (2013) Induction of chondrogenic differentiation in mesenchymal stem cells by TGF-beta cross-linked to collagen-PLLA [poly(L-lactic acid)] scaffold by transglutaminase 2. Biotechnol Lett 35:2193–2199
Amali AJ, Awwad NH, Rana RK et al (2011) Nanoparticle assembled microcapsules for application as pH and ammonia sensor. Anal Chim Acta 708:75–83
Kurayama F, Suzuki S, Oyamada T et al (2010) Facile method for preparing organic/inorganic hybrid capsules using amino-functional silane coupling agent in aqueous media. J Colloid Interface Sci 349:70–76
Jean M, Alameh M, De Jesus D et al (2012) Chitosan-based therapeutic nanoparticles for combination gene therapy and gene silencing of in vitro cell lines relevant to type 2 diabetes. Eur J Pharm Sci 45:138–149
Pujana MA, Pérez-Álvarez L, Iturbe LCC et al (2012) Water dispersible pH-responsive chitosan nanogels modified with biocompatible crosslinking-agents. Polymer 53:3107–3116
Acknowledgements
This work was financially supported by the Portuguese Foundation for Science and Technology (FCT) through the project PTDC/CTM-Bio/1814/2012 and Ph.D. grant with the reference no. SFRH/BD/69529/2010, co-financed by the Operational Human Potential Program (POPH), developed under the scope of the National Strategic Reference Framework (QREN) from the European Social Fund (FSE). The authors also acknowledge the financial support from Seventh Framework Programme of European Union (FP7/2007–2013) under grant agreement number REGPOT-CT2012-316331-POLARIS.
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Correia, C.R., Reis, R.L., Mano, J.F. (2015). Nanostructured Capsules for Cartilage Tissue Engineering. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_13
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DOI: https://doi.org/10.1007/978-1-4939-2938-2_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2937-5
Online ISBN: 978-1-4939-2938-2
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