Abstract
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
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Acknowledgements
The authors thank Simon Bartlett for editorial assistance. Work in VA’s laboratory is supported by grants SAF2013-46663-R and RD12/0042/0028 from the Spanish Ministerio de Economía y Competitividad (MINECO) with co-funding from the Fondo Europeo de Desarrollo Regional (FEDER), the European Commission (Liphos, Grant Agreement No. 317916), and the Progeria Research Foundation (Established Investigator Award). The CNIC is supported by the MINECO and the Pro-CNIC Foundation.
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Andrés-Manzano, M.J., Andrés, V., Dorado, B. (2015). Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_5
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DOI: https://doi.org/10.1007/978-1-4939-2929-0_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2928-3
Online ISBN: 978-1-4939-2929-0
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