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Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity

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Cell-Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1324))

Abstract

In the study of cell-penetrating and membrane-translocating peptides, a fundamental question occurs as to the contribution arising from fundamental peptide–membrane interactions, relative to the contribution arising from the biology and energy of the cell, mostly occurring in the form of endocytosis and subsequent events. A commonly used approach to begin addressing these mechanistic questions is to measure the degree to which peptides can interact with, and physically disrupt, the integrity of synthetic lipid bilayers. Here, we describe a set of experimental methods that can be used to measure the potency, kinetics, transience, and the effective size of peptide-induced membrane disruption.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave #8543, New Orleans, LA, 70112, USA

    William C. Wimley

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  1. William C. Wimley
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Corresponding author

Correspondence to William C. Wimley .

Editor information

Editors and Affiliations

  1. Department of Neurochemistry, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Wimley, W.C. (2015). Determining the Effects of Membrane-Interacting Peptides on Membrane Integrity. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2806-4_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2805-7

  • Online ISBN: 978-1-4939-2806-4

  • eBook Packages: Springer Protocols

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