Abstract
Carrageenans are sulfated galactan isolated from marine red algae with different disaccharide forms. There are also some hybrid carrageenan-like oligomers, which are reported to possess a number of bioactivities. Here, we describe a method to study the structural characterization of a carrageenan-like sulfated galactan FB1 extracted from the red seaweed Furcellaria lumbricalis. We show the process of the general analysis of FB1, including the molecular weight, sulfate content, total sugar content, protein content, and 3,6-anhydrogalactose (3,6-AnG) content analyses. The fine structure identification methods, including desulfation and methylation, nuclear magnetic resonance (NMR), and electrospray ionization collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS), are also described in detail.
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Acknowledgements
This research is supported in part by Special Fund for Marine Scientific Research in the Public Interest (201005024), National Science & Technology Support Program of China (2013BAB01B02), and the NSFC-Shandong Joint Fund for Marine Science Research Centers (U1406402). It is based on a collaborative research supported by Ocean Nutrition Canada Ltd. and the Atlantic Canada Opportunity Agency (ACOA), funded through the PEI Food Technology Center.
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Lv, Y., Yang, B., Zhao, X., Zhang, J., Yu, G. (2015). Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis . In: Stengel, D., Connan, S. (eds) Natural Products From Marine Algae. Methods in Molecular Biology, vol 1308. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2684-8_21
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DOI: https://doi.org/10.1007/978-1-4939-2684-8_21
Publisher Name: Humana Press, New York, NY
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