Skip to main content
SpringerLink
Log in
Book cover

Glycosaminoglycans pp 557–565Cite as

Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Nanoparticles are being explored for a variety of applications including medical imaging, drug delivery, and biochemical detection. Surface functionalization of nanoparticles with glycosaminoglycans (GAGs) is an attractive strategy that is only starting to be investigated to improve their properties for biological and therapeutic applications. Herein, we describe a method to functionalize the surface of cerium oxide nanoparticles (nanoceria) with organosilane linkers, such as 3-(aminopropyl)triethoxysilane (APTES) and 3-(mercaptopropyl)trimethoxysilane (MPTMS), and GAGs, such as unfractionated and low molecular weight heparin. Examples of how the activity of these heparin functionalized nanoparticles are governed by the pendant GAGs are detailed. The activity of heparin covalently attached to the nanoceria was found to be unchanged when compared to unfractionated heparin using the activated partial clotting time (APTT) assay.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Reyes-Ortega F, Rodríguez G, Aguilar MR, Lord M, Whitelock J, Stenzel MH, San Román J (2013) Encapsulation of low molecular heparin (bemiparin) into polymeric nanoparticles obtained from cationic block copolymers: properties and cell activity. J Mater Chem B 1:850–860

    Article  CAS  Google Scholar 

  2. Zhao Y, Lord MS, Stenzel MH (2013) A polyion complex micelle with heparin for growth factor delivery and uptake into cells. J Mater Chem B 1:1635–1643

    Article  CAS  Google Scholar 

  3. Ting SRS, Whitelock JM, Tomic R, Gunawan C, Teoh WY, Amal R et al (2013) Cellular uptake and activity of heparin functionalised cerium oxide nanoparticles in monocytes. Biomaterials 34:4377–44386

    Article  CAS  PubMed  Google Scholar 

  4. Lord MS, Tsoi B, Gunawan C, Teoh WY, Amal R, Whitelock JM (2013) Anti-angiogenic activity of heparin functionalised cerium oxide nanoparticles. Biomaterials 28:8888–8999

    Google Scholar 

  5. Gandhi NS, Mancera RL (2008) The structure of glycosaminoglycans and their interactions with proteins. Chem Biol Drug Des 72:455–482

    Article  CAS  PubMed  Google Scholar 

  6. Yuk SH, Oh KS, Cho SH, Lee BS, Kim SY, Kwak BK et al (2011) Glycol chitosan/heparin immobilized iron oxide nanoparticles with a tumor-targeting characteristic for magnetic resonance imaging. Biomacromolecules 12:2335–2343

    Article  CAS  PubMed  Google Scholar 

  7. Lord MS, Jung M, Teoh WY, Gunawan C, Vassie JA, Amal R et al (2012) Cellular uptake and reactive oxygen species modulation of cerium oxide nanoparticles in human monocyte cell line U937. Biomaterials 33:7915–7924

    Article  CAS  PubMed  Google Scholar 

  8. Nilasaroya A, Poole-Warren LA, Whitelock JM, Martens PJ (2008) Structural and functional characterisation of poly(vinyl alcohol) and heparin hydrogels. Biomaterials 29:4658–4664

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work was supported by the Australian Research Council Discovery Project scheme.

Author information

Authors and Affiliations

  1. Graduate School of Biomedical Engineering, The University of New South Wales, Sydney, NSW, 2052, Australia

    James A. Vassie, John M. Whitelock & Megan S. Lord

Authors
  1. James A. Vassie
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John M. Whitelock
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Megan S. Lord
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Megan S. Lord .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Vassie, J.A., Whitelock, J.M., Lord, M.S. (2015). Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_44

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_44

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation