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Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration

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Glycosaminoglycans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1229))

Abstract

Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

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Acknowledgement

This work was supported by grants from NIH R01HL093339 (L.W.) and RR005351/GM103390 (L.W.).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA, 30602-4712, USA

    Hong Qiu, Wenyuan Xiao, Jingwen Yue & Lianchun Wang M.D.

Authors
  1. Hong Qiu
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  2. Wenyuan Xiao
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  3. Jingwen Yue
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  4. Lianchun Wang M.D.
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Corresponding author

Correspondence to Lianchun Wang M.D. .

Editor information

Editors and Affiliations

  1. Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA

    Kuberan Balagurunathan

  2. Department of Genetics, Cell Biology and Development, The University of Minnesota, Minneapolis, Minnesota, USA

    Hiroshi Nakato

  3. Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, Virginia, USA

    Umesh R. Desai

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Qiu, H., Xiao, W., Yue, J., Wang, L. (2015). Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration. In: Balagurunathan, K., Nakato, H., Desai, U. (eds) Glycosaminoglycans. Methods in Molecular Biology, vol 1229. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1714-3_43

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  • DOI: https://doi.org/10.1007/978-1-4939-1714-3_43

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1713-6

  • Online ISBN: 978-1-4939-1714-3

  • eBook Packages: Springer Protocols

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