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Isolation of Leukocytes from Mouse Central Nervous System

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1193))

Abstract

During neuroinflammatory or neurodegenerative diseases, it is often critical to characterize the composition of infiltrating immune cells. This protocol describes a reliable, fast, and simple method for the isolation of leukocytes from murine central nervous system (CNS) during steady state or inflammation for analysis by flow cytometry or other techniques.

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References

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Correspondence to Melanie Greter .

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© 2014 Springer Science+Business Media New York

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Lelios, I., Greter, M. (2014). Isolation of Leukocytes from Mouse Central Nervous System. In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-1212-4_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1211-7

  • Online ISBN: 978-1-4939-1212-4

  • eBook Packages: Springer Protocols

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