Abstract
In the last decades there has been a substantial increase in the prevalence of allergic diseases such as asthma. Hence there is a basic necessity to investigate disease mechanisms of allergic disorders and to trace novel treatment approaches. Indeed, allergic asthma is a disorder which is characterized by airway inflammation, airway obstruction, and hyperresponsiveness. Several of these features can be studied in models of allergic airway disease. In this chapter different mouse models of allergic diseases are described. These include frequently models of allergic airway disease utilizing sensitization and challenge towards ovalbumin. Furthermore a model using the human-relevant allergen-specific house dust mite is described. In addition, information about DC-induced models and analysis of in vitro-generated T cell following transfer into recipients are described as well as analysis of a humanized mouse model is provided.
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References
Hammad H, Chieppa M, Perros F, Willart MA, Germain RN, Lambrecht BN (2009) House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells. Nat Med 15:410
Nigo YI, Yamashita M, Hirahara K, Shinnakasu R, Inami M, Kimura M, Hasegawa A, Kohno Y, Nakayama T (2006) Regulation of allergic airway inflammation through Toll-like receptor 4-mediated modification of mast cell function. Proc Natl Acad Sci U S A 103:2286
Willart MA, Deswarte K, Pouliot P, Braun H, Beyaert R, Lambrecht BN, Hammad H (2012) Interleukin-1 alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J Exp Med 209:1505
Idzko M, Hammad H, van Nimwegen M, Kool M, Vos N, Hoogsteden HC, Lambrecht BN (2007) Inhaled iloprost suppresses the cardinal features of asthma via inhibition of airway dendritic cell function. J Clin Invest 117:464
Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Tureci O, Grabbe S, Taube C, Saloga J (2012) Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol 130:1384
Martin H, Reuter S, Dehzad N, Heinz A, Bellinghausen I, Saloga J, Haasler I, Korn S, Jonuleit H, Buhl R, Becker C, Taube C (2012) CD4-mediated regulatory T-cell activation inhibits the development of disease in a humanized mouse model of allergic airway disease. J Allergy Clin Immunol 129(521):528
Duez C, Kips J, Pestel J, Tournoy K, Tonnel AB, Pauwels R (2000) House dust mite-induced airway changes in hu-SCID mice. Am J Respir Crit Care Med 161:200
Hammad H, Lambrecht BN, Pochard P, Gosset P, Marquillies P, Tonnel AB, Pestel J (2002) Monocyte-derived dendritic cells induce a house dust mite-specific Th2 allergic inflammation in the lung of humanized SCID mice: involvement of CCR7. J Immunol 169:1524
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Meyer-Martin, H., Reuter, S., Taube, C. (2014). Mouse Models of Allergic Airway Disease. In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_13
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DOI: https://doi.org/10.1007/978-1-4939-1212-4_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1211-7
Online ISBN: 978-1-4939-1212-4
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