Abstract
The actin cytoskeleton plays a fundamental role in controlling several steps during regulated exocytosis. Here, we describe a combination of procedures that are aimed at studying the dynamics and the mechanism of the actin cytoskeleton in the salivary glands of live rodents, a model for exocrine secretion. Our approach relies on intravital microscopy, an imaging technique that enables imaging biological events in live animals at a subcellular resolution, and it is complemented by the use of pharmacological agents and indirect immunofluorescence in the salivary tissue.
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Acknowledgments
This research was supported by the Intramural Research Program of the NIH, National Institute of Dental and Craniofacial Research.
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Milberg, O., Tora, M., Shitara, A., Takuma, T., Masedunskas, A., Weigert, R. (2014). Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy. In: Ivanov, A. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 1174. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0944-5_28
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DOI: https://doi.org/10.1007/978-1-4939-0944-5_28
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