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Identification of Transcription Factors Activated in Thymic Epithelial Cells During Embryonic Thymus Development

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Transcription Factor Regulatory Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1164))

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Abstract

Differentiation of many immune-related cells is controlled by the expression levels and the activation status of transcription factors (TFs). We here describe a method to identify candidate TFs activated during the development of thymic epithelial cells (TECs) in the embryo. RNAs are isolated from fetal thymic organ cultures of wild-type and mutant mice and are subsequently analyzed by using a combination of comprehensive expression analysis and in silico data analysis in order to predict the TFs that might be activated.

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Acknowledgement

This work was supported by a grant from the Genome Network Project from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Division of Cellular and Molecular Biology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo, 108-8639, Japan

    Taishin Akiyama & Jun-ichiro Inoue

  2. Key Laboratory for Regenerative Medicine, Department of Developmental and Regenerative Biology, Ministry of Education and International Base of Collaboration for Science and Technology, The Ministry of Science and Technology and Guangdong Province, Jinan University, Guangzhou, 510632, China

    Junwen Qin

  3. Division of Mathematical Oncology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokane-dai, Minato-ku, Tokyo, 108-8639, Japan

    Daisuke Ohshima

Authors
  1. Taishin Akiyama
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  2. Junwen Qin
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  3. Daisuke Ohshima
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  4. Jun-ichiro Inoue
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Corresponding authors

Correspondence to Taishin Akiyama or Jun-ichiro Inoue .

Editor information

Editors and Affiliations

  1. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Etsuko Miyamoto-Sato

  2. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hiroyuki Ohashi

  3. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hirotaka Sasaki

  4. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Jun-ichi Nishikawa

  5. Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan

    Hiroshi Yanagawa

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Akiyama, T., Qin, J., Ohshima, D., Inoue, Ji. (2014). Identification of Transcription Factors Activated in Thymic Epithelial Cells During Embryonic Thymus Development. In: Miyamoto-Sato, E., Ohashi, H., Sasaki, H., Nishikawa, Ji., Yanagawa, H. (eds) Transcription Factor Regulatory Networks. Methods in Molecular Biology, vol 1164. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0805-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-0805-9_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0804-2

  • Online ISBN: 978-1-4939-0805-9

  • eBook Packages: Springer Protocols

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