Abstract
A challenge facing surgeons is identification and selection of patients for carotid endarterectomy or coronary artery bypass/surgical intervention. While some patients with atherosclerosis develop unstable plaques liable to undergo thrombosis, others form more stable plaques and are asymptomatic. Identification of the cellular signaling mechanisms associated with production of the inflammatory, hemorrhagic lesions of mature heterogenic plaques will help significantly in our understanding of the differences in microenvironment associated with development of regions susceptible to rupture and thrombosis and may help to predict the risk of plaque rupture and guide surgical intervention to patients who will most benefit. Here, we demonstrate detailed and novel methodologies for successful and, more importantly, accurate and reproducible extraction, sampling, and analysis of micro-regions in stable and unstable coronary/carotid arteries. This information can be applied to samples from other origins and so should be useful for scientists working with micro-isolation techniques in all fields of biomedical science.
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Acknowledgments
We would like to thank the PIV and the Foundation BBVA for their generous support of Professor Mark Slevin through the award of BBVA Chair in Clinical Biomedicine at the ICCC, St Pau Hospital, Barcelona. This work was also supported by the Spanish Ministry of Science (SAF2006-10091 to L.B.), Instituto de Salud Carlos III (CIBEROBN-CB06/03 to L.B.), and RyC (RyC2007-01466 to M.B.-P.). Maribel Baldellou and Lina Badimon have received funding by the network REDINSCOR RD06/0003/0015.
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Slevin, M. et al. (2014). Novel Methods for Accurate Identification, Isolation, and Genomic Analysis of Symptomatic Microenvironments in Atherosclerotic Arteries. In: Milner, R. (eds) Cerebral Angiogenesis. Methods in Molecular Biology, vol 1135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0320-7_24
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DOI: https://doi.org/10.1007/978-1-4939-0320-7_24
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0319-1
Online ISBN: 978-1-4939-0320-7
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