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Saccharomyces pp 283–320Cite as

Biochemical Techniques

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Part of the book series: Biotechnology Handbooks ((BTHA,volume 4))

Abstract

A plethora of biochemical techniques have been developed to study fundamental cellular processes of yeasts of the Saccharomyces genera. All of these generally require efficient disruption of the yeast cell followed either by purification of particular classes of macromolecules (e.g., proteins, nucleic acids, ribonucleoprotein complexes) or by partial fractionation of the lysate to yield subfractions that are particularly enriched in some biosynthetic activity, be it a given enzyme or a multicomponent process such as protein synthesis. In this chapter we describe the basic strategies that have been developed for Saccharomyces species (especially S. cerevisiae) for both cell disruption and fractionation, together with consideration of how one radiolabels specific macromolecules in vivo. A detailed account of strategies for subcellular fractionation of yeast cell components—mitochondria, membranes, vacuoles, and so forth—can be found in Chapter 2.

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Authors and Affiliations

  1. Biological Laboratory, University of Kent, Canterbury, Kent, CT2 7NJ, England

    Michael F. Tuite

  2. Institute for Science and Technology, Manchester Biotechnology Centre, University of Manchester, Manchester, M60 1QD, England

    Stephen G. Oliver

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  1. Michael F. Tuite
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  2. Stephen G. Oliver
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  1. Biological Laboratory, University of Kent, CT2 7NJ, Canterbury, Kent, England

    Michael F. Tuite

  2. Manchester Biotechnology Centre, Institute for Science and Technology, University of Manchester, M60 1QD, Manchester, England

    Stephen G. Oliver

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© 1991 Springer Science+Business Media New York

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Tuite, M.F., Oliver, S.G. (1991). Biochemical Techniques. In: Tuite, M.F., Oliver, S.G. (eds) Saccharomyces. Biotechnology Handbooks, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2641-8_9

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  • DOI: https://doi.org/10.1007/978-1-4899-2641-8_9

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