Abstract
Samples of hard wheat flour and dough are analyzed by magic-angle spinning 13C NMR spectroscopy. Cross-polarization magic-angle spinning (CPMAS) 13C NMR spectra of the dry flour allow its starch and protein content to be accurately measured. These two components are phase-separated. Spectra of hydrated hard wheat doughs are collected under both CPMAS and single-pulse carbon with low-power 1H decoupling conditions. The former report on the macromolecular components of the dough, while the latter reveal small molecules which are solubilized by the water. Results of the present study are interpreted as indicating that the protein is largely unaffected by the added water and remains phased-separated from the starch, while water causes significant changes in polymer dynamics of the starch component.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
S.A. Matz, Modem baking technology, Scientific American November: 122 (1984).
D.L. Laidman and R.G. Wyn Jones, eds., “Recent Advances in the Biochemistry of Cereals,” Academic Press, New York (1979).
W.M. Shirley and R.G. Bryant, Proton-nuclear spin relaxation and molecular dynamics in the lysozyme-water system, J. Am. Chem. Soc. 104:2910 (1982).
W.B. Wise and P.E. Pfeffer, Measurement of cross-relaxation effects in the proton NMR of water in fibrous collagen and insoluble elastin, Macromolecules 20:1550 (1987).
R.A. Komoroski, ed., “High Resolution NMR Spectroscopy of Synthetic Polymers in Bulk,” VCH, Deerfield Beach (1986).
V.D. Fedotov and H. Schneider, “Structure and Dynamics of Bulk Polymers by NMR-Methods,” Springer-Verlag, Heidelberg (1989).
J. Schaefer, E.O. Stejskal, R.A. McKay, and W.T. Dixon, Molecular motion in polycarbonates by dipolar rotational spin-echo 13C NMR, Macromolecules 17:1479 (1984).
J. Schaefer and E.O. Stejskal, Carbon-13 nuclear magnetic resonance of polymers spinning at the magic angle, J. Am. Chem. Soc. 98:1031 (1976).
R.H. Atalla, J.C. Gast, D.V. Sindorf, V.J. Bartuska, and G.E. Maciel, 13C NMR spectra of cellulose polymorphs, J. Am. Chem. Soc. 102:3249 (1980).
W.L. Earl and D.L. VanderHart, High resolution magic angle sample spinning 13C NMR of solid cellulose I, J. Am. Chem. Soc. 102:3251 (1984).
J. Schaefer, M.D. Sefcik, E.O. Stejskal, R.A. McKay, and P.L. Hall, Characterization of the catabolic transformation of lignin in culture using magic-angle carbon-13 nuclear magnetic resonance, Macromolecules 14:557 (1980).
G.E. Maciel, J.F. Haw, D.H. Smith, B.C. Gabrielson, and G.R. Hatfield, Carbon-13 nuclear magnetic resonance of herbaceous plants and their components, using cross polarization and magic-angle spinning, J. Agric. Food Chem. 33:185 (1985).
N.G. Lewis, J. Newman, G. Just, and J. Ripmeister, Determination of bonding patterns of 13C specifically enriched dehydrogenatively polymerized lignin in solution and solid state, Macromolecules 20:1752 (1987).
R. Botto, Synthesis and characterization of [13C] lignins. Macromolecules 21:1246 (1988).
T. Zlotnik-Mazori and R.E. Stark, Nuclear magnetic resonance studies of cutin, an insoluble plant polyester, Macromolecules 21:2412 (1988).
R.E. Stark, T. Zlotnik-Mazori, D.M. Ferrantello, and J.R. Garbow, Molecular structure and dynamics of intact plant polyesters: solid-state NMR studies, in: “Plant Cell Wall Polymers, Biogenesis and Biodegradation,” N.G. Lewis and M.G. Paice, eds., ACS, Washington, DC (1989).
J.R. Garbow, L.M. Ferrantello, and R.E. Stark, 13C nuclear magnetic resonance study of suberized potato cell wall, Plant Physiology 90:783 (1989).
E.O. Stejskal, J. Schaefer, and T.R. Steger, High-resolution 13C nuclear magnetic resonance in solids, Faraday Disc. Chem. Soc. 13:56 (1979).
E.O. Stejskal, J. Schaefer, M.D. Sefcik, and R.A. McKay, Magic-angle carbon-13 nuclear magnetic resonance study of the compatibility of solid polymeric blends, Macromolecules 14:275 (1981).
J. Schaefer, J.R. Garbow, E.O. Stejskal, and J. Lefalar, Plasticization of butvar resins, Macromolecules 20:1271 (1987).
A. Pines, M.G. Gibby, and J.S. Waugh, Proton-enhanced NMR of dilute spins in solids, J. Chem. Phys. 59:569 (1973).
E.R. Andrew, A. Bradbury, and R.G. Eades, Nuclear magnetic resonance spectra from a crystal rotated at high speed, Nature 182:1659 (1958).
I.J. Lowe, Free induction decays of rotating solids, Phvs. Rev. Lett. 2:285 (1959).
J.R. Havens and D.L. VanderHart, Morphology of poly(ethylene terephthalate) fibers as studied by multiple-pulse 1H NMR, Macromolecules 18:1663 (1985).
W. Rothwell and J. Waugh, Transverse relaxation of dipolar coupled spin systems under r.f. irradiation: detecting motions in solids, J. Chem. Phvs. 74:2721 (1981).
J.R. Garbow, J. Schaefer, E.O. Stejskal, and G.S. Jacob, Protein dynamics from chemical shift and dipolar rotational spin-echo 15N NMR, Biochemistry 28:1362 (1989).
J.R. Garbow and J. Schaefer, J. Agric. Food Chem.. in press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Garbow, J.R., Schaefer, J. (1991). Magic-Angle 13C NMR Analysis of Hard Wheat Flour and Dough. In: Levine, H., Slade, L. (eds) Water Relationships in Foods. Advances in Experimental Medicine and Biology, vol 302. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0664-9_27
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0664-9_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0666-3
Online ISBN: 978-1-4899-0664-9
eBook Packages: Springer Book Archive