Abstract
The ultimate goal of genetic analysis of the mammalian reoviruses is to gain a detailed understanding of the structure and function of the genome and each of the viral polypeptide products. The early phases of genetic analysis were directed toward the isolation and physiological and genetic characterization of a collection of genetic markers, in this case, conditional lethal mutants of the temperature-sensitive type. More recent studies, particularly those since 1975, have emphasized the use of recombinant viruses generated during mixed infection of tissue-culture cells with pairs of viruses, most often of two different reovirus serotypes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ahmed, R., and Graham, A.F., 1977, Persistent infection in L cells with temperature-sensitive mutants of reovirus, J. Virol. 23:250–262.
Ahmed R., Chakraborty, P.R., and Fields, B.N., 1980a, Genetic variation during lytic reovirus infection: High passage stocks of wild type reovirus contain temperature-sensitive mutants, J. Virol. 34:285–287.
Ahmed, R., Chakraborty, P.R., Graham, A.F., Ramig, R.F., and Fields, B.N., 1980b, Genetic variation during persistent reovirus infection: Presence of extragenically suppressed temperature-sensitive lesions in wild type virus isolated from persistently infection L cells, J. Virol. 34:383–389.
Ahmed, R., and Fields, B.N., 1981, Reassortment of genome segments between reovirus defective interfering particles and infectious virus: Construction of temperature-sensitive and attenuated viruses by rescue of mutations from DI particles, Virology 111:351–363.
Ahmed, R., Canning, W.M., Kauffman, R.S., Sharpe, A.H., Hallum, J.V., and Fields, B.N., 1981, Role of the host cell in persistent viral infection: Coevolution of L cells and reovirus during persistent infection, Cell 25:325–332.
Chakraborty, P.R., Ahmed, R., and Fields, B.N., 1979, Genetics of reovirus: The relationship of interference to complementation and reassortment of temperature-sensitive mutants at nonpermissive temperature, Virology 94:119–127.
Cooper, P.D., 1968, A genetic map of poliovirus temperature-sensitive mutants, Virology 35:584–596.
Cross, R.K., and Fields, B.N., 1972, RNA synthesized in cells infected with reovirus type 3 mutants, Virology 50:799–809.
Cross, R.K., and Fields, B.N., 1976a, Temperature-sensitive mutants of reovirus type 3: Evidence for aberrant (μ1 and μ2 polypeptide species. J. Virol. 19:174–179.
Cross, R.K., and Fields, B.N., 1976b, Use of an aberrant polypeptide as a marker in three-factor crosses: Further evidence for independent reassortment as the mechanism of recombination between temperature-sensitive mutants of reovirus type 3, Virology 74:345–362.
Cross, R.K., and Fields, B.N., 1976c, Reovirus-specific polypeptides: Analysis using discontinuous gel electrophoresis, J. Virol. 19:162–173.
Fields, B.N., 1971. Temperature-sensitive mutants of reovirus—features of genetic recombination, Virology 46:142–148.
Fields, B.N., 1973, Genetic reassortment with reovirus mutants, in: Virus Research (C.F. Fox, ed.), p. 461, Academic Press, New York.
Fields, B.N., and Joklik, W.K., 1969, Isolation and preliminary genetic and biochemical characterization of temperature-sensitive mutants of reovirus, Virology 37:335–342.
Fields, B.N., Raine, C.S., and Baum, S.G., 1971, Temperature-sensitive mutants of reovirus type 3: Defects in virus maturation as studied by immunofluorescence electron microscopy, Virology 43:569–578.
Floor, E., 1970, Interaction of morphogenetic genes of bacteriophage T4, J. Mol. Biol. 47:293–306.
Gomatos, P.J., Tamm, I., Dales, S., and Franklin, R.M., 1962, Reovirus type 3: Physical characteristics and interactions with L cells, Virology 17:441.
Gomatos, P.J., Prakash, O., and Stamatos, N.M., 1981, Small reovirus particles composed solely of sigma NS with specificity for binding different nucleic acids. J. Virol. 39:115–124.
Hrdy, D.B., Rosen, L., and Fields, B.N., 1979, Polymorphism of the migration of double stranded RNA genome segments of reovirus isolates from humans, cattle and mice, J. Virol. 31:104–111.
Huismans, H., and Joklik, W.K., 1976, Reovirus-coded polypeptides in infected cells: Isolation of two native monomeric polypeptides for single stranded and double stranded RNA. Virology 70:411–424.
Ikegami, N., and Gomatos, P.J., 1968, Temperature-sensitive conditional lethal mutants of reovirus 3.1. Isolation and characterization, Virology 36:447.
Ikegami, N., and Gomatos, P.J., 1972, Inhibition of host and viral protein synthesis during infection at the nonpermissive temperature with ts mutants of reovirus 3, Virology 47:306–312.
Ito, Y., and Joklik, W.K., 1972a, Temperature-sensitive mutants of reovirus. II. Anomalous electrophoretic migration behavior of certain molecules composed of mutant plus strands and wild type minus strands, Virology 50:202–208.
Ito, Y., and Joklik, W.K., 1972b, Temperature-sensitive mutants of reovirus. I. Patterns of gene expression by mutants of groups C., D and E, Virology 50:189–201.
Ito, Y., and Joklik, W.K., 1972c, Temperature-sensitive mutants of reovirus. III. Evidence that mutants of group C (“RNA negative”) are structural polypeptide mutants, Virology 50:282–286.
Jarvik, J., and Botstein, D., 1975, Conditional-lethal mutations that suppress defects in morphogenesis by altering structural proteins, Proc. Natl. Acad. Sci. U.S.A. 72:2738–2742.
Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature (London) 227:680–685.
Matsuhisa, T., and Joklik, W.K., 1974, Temperature-sensitive mutants of reovirus. V. Studies on the nature of the temperature sensitive lesion of the C group mutant ts447, Virology 60:380–389.
McCrae, M.A., and Joklik, W.K., 1978, The nature of the polypeptide encoded by each of the ten double-stranded RNA segments of reovirus type 3, Virology 89:578–593.
Morgan, E.M., and Zweerink, H.J., 1974, Reovirus morphogenesis: Core-like particles in cells infected at 39 with wild type and temperature sensitive mutants of groups B and G, Virology 59:556–565.
Murphy, B.R., Tolpin, M.D., Massicot, J.G., Kim, H.Y., Parrott, R.H., and Chanock, R.M., 1980, Escape of a highly defective influenza A virus mutant from its temperature sensitive phenotype by extragenic suppression and other types of mutation, Ann. N.Y. Acad. Sci. 354:172–182.
Mustoe, T.A., Ramig, R.F., Sharpe, A.H., and Fields, B.N., 1978a, A genetic map of reovirus. III. Assignment of the double-stranded RNA positive mutant groups, A, B and G to genome segments, Virology 85:545–556.
Mustoe, T.A., Ramig, R.F., Sharpe, A.H., and Fields, B.N., 1978b, Genetics of reovirus: Identification of the dsRNA segments encoding the polypeptides of the mu and sigma size classes, Virology 89:594–604.
Nonoyama, M., and Graham, A.F., 1970, Appearance of defective virions in clones of reovirus, J. Virol. 6:693.
Prevec, L., Watanabe, Y., Gauntt, C.J., and Graham, A.F., 1968, Transcription of the genomes of type 1 and type 3 reovirus, J. Virol. 2:289.
Ramig, R.F., 1980, Suppression of temperature sensitive phenotype in reovirus: An alternative pathway from ts to ts+ phenotype, in: Animal Virus Genetics (B.N. Fields, R. Jaenisch, and C.F. Fox, eds.), pp. 633–642, Academic Press, New York.
Ramig, R.F., and Fields, B.N., 1977, Method for rapidly screening revenants of reovirus temperatures sensitive mutants for extragenic suppression, Virology 81:170–173.
Ramig, R.F., and Fields, B.N., 1979, Revenants of temperature sensitive mutants of reovirus: Evidence for frequent extragenic suppression, Virology 92:155–167.
Ramig, R.F., White, R.M., and Fields, B.N., 1977a, Suppression of the temperature sensitive phenotype of a mutant of reovirus type 3, Science 195:406–407.
Ramig, R.F., Cross, R.K., and Fields, B.N., 1977b, Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3, J. Virol. 22:726–733.
Ramig, R.F., Mustoe, T.A., Sharpe, A.H., and Fields, B.N., 1978, A genetic map of reovirus. II. Assignment of the double stranded RNA negative mutant groups C, D and E to genome segments, Virology 85:531–544.
Ramig, R.F., Ahmed, R., and Fields, B.N., 1983, A genetic map of reovirus: Assignment of the newly defined mutant groups H, I and J to genome segments (in press).
Rubin, D.H., and Fields, B.N., 1980, Molecular basis of reovirus virulence: Role of the M2 gene, J. Exp. Med. 152:853–868.
Schuerch, A.R., and Joklik, W.K., 1973, Temperature-sensitive mutants of reovirus. IV. Evidence that anomalous electrophoretic migration behavior of certain double stranded RNA hybrid species is mutant group specific, Virology 56:218–229.
Schuerch, A.R., Matsuhisa, T., and Joklik, W.K., 1974, Temperature-sensitive mutants of reovirus. VI. Mutant ts 447 and ts 556 particles that lack either one or two genome segments, Intervirology 3:36–46.
Sharpe, A.H., Ramig, R.F., Mustoe, T.A., and Fields, B.N., 1978, A genetic map of reovirus. I. Correlation of genome RNAs between serotype 1, 2 and 3, Virology 84:63–74.
Shatkin, A.J., Sipe, J.D., and Loh, P., 1968, Separation of 10 reovirus segments by polyac-rylamide gel electrophoresis, J. Virol. 2:968.
Skehel, J.J., and Joklik, W.K., 1969, Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores, Virology 39:822.
Smith, R.E., Zweerink, H.J., and Joklik, W.K., 1969, Polypeptide components of virions, top component and cores of reovirus type 3, Virology 39:791–810.
Spandidos, D.A., and Graham, A.F., 1975a, Complementation of defective reovirus by ts mutants, J. Virol. 15:954–963.
Spandidos, D.A., and Graham, A.F., 1975b, Complementation between temperature-sensitive and deletion mutants of reovirus, J. Virol. 16:1444–1453.
Spandidos, D.A., and Graham, A.F., 1976, Recombination between temperature sensitive and deletion mutants of reovirus, J. Virol. 18:117–123.
Tolpin, M.D., Massicot, J.G., Mullinix, M.G., Kim, H.W., Parrott, R.H., Chanock, R.M., and Murphy, B.R., 1981, Genetic factors associated with loss of the temperature sensitive phenotype of the influenza A/Alaska/77-ts-1A2 recombinant during growth in vivo, Virology 112:505–517.
Tolpin, M.D., Clements, MX., Levine, M.M., Black, R.E., Saah, A.J., Anthony, W.C., Cisneros, L., Chanock, R.M., and Murphy, B.R., 1982, Evaluation of a phenotypic revenant of the A/Alaska/77-ts-lA2 reassortment virus in hamsters and in seronegative adult volunteers: Further evidence that the temperature sensitive phenotype is responsible for attenuation of ts-lA2 reassortant viruses, Infect. Immun. 36:645–650.
Weiner, H.L., and Fields, B.N., 1977, Neutralization of reovirus; The gene responsible for the neutralization antigen, J. Exp. Med. 146:1303–1310.
Weiner, H.L., Ramig, R.F., Mustoe, T.A., and Fields, B.N., 1978, Identification of the gene coding for the hemagglutinin of reovirus, Virology 86:581–584.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1983 Springer Science+Business Media New York
About this chapter
Cite this chapter
Ramig, R.F., Fields, B.N. (1983). Genetics of Reoviruses. In: Joklik, W.K. (eds) The Reoviridae. The Viruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-0580-2_5
Download citation
DOI: https://doi.org/10.1007/978-1-4899-0580-2_5
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4899-0582-6
Online ISBN: 978-1-4899-0580-2
eBook Packages: Springer Book Archive