Abstract
The employment of immobilized antibodies (antigens) offers the most selective approach to extracorporeal adsorptive removal of humoral factors from blood. Polypeptide mediators and antibodies in systemic inflammatory and autoimmune diseases, respectively, can not be depleted by hemodialysis treatment, and thus represent attractive targets for immunoadsorption regimen. In this article we describe the elimination of interleukin 1β (IL-1β) and tumor necrosis factor α (TNFα), the major pathogenic mediators in septic shock and multi-organ failure, by superparamagnetic microspheres used in the novel MDS blood purification technology. The particles, consisting of antibody conjugates, exhibit high adsorption rate, capacity, selectivity, and biocompatibility. By application in an in vitro MDS set-up, human plasma could be efficiently cleansed from IL-lß and TNFa. Contrary to conventional blood and plasma chromatography techniques, MDS offers a high degree of flexibility and continuous blood processing due to magnetic adsorbent replacement.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Yokayama S, Hayashi R, Satani M, Yamamoto (1985). Selective removal of LDL by plasmapheresis in familial hypercholesterolemia. Arteriosclerosis 5, 613–622.
Usami M, Nomura H, Nishimatsu S, Shiroiwa H, Kasahara H, Takeyama Y, Saitoh Y (1995). In vivo and in vitro evaluation of bilirubin removal in postoperative hyperbilirubinemia patients. Artif Organs 19, 102–105.
Nagesh RV and Murphy KA (1988). Caffeine poisoning treated by hemoperfusion. Am J Kid Dis 12, 316–318.
Heininger K, Gaczkowski A, Hartung HP, Borberg H, Toyka KV (1987). A new semiselective procedure in therapeutic plasma exchange for myasthenia gravis. In Therapeutic plasma exchange and selective plasma separation. Bambauer R, Malchesky PS, Falkenhagen D (Eds), Stuttgart, Schattauer, 393–398.
Yuki N (1996). Tryptophan-immobilized column adsorbs immunoglobulin G anti-GQ1b antibody from Fisher’s syndrome: a new approach to treatment. Neurology 46, 1644–1651.
Grob D, Simpson D, Mitsumoto H, Hoch B, Mokhtarian F, Bender A, Greenberg M, Koo A, Nakayama S (1995). Treatment of myasthenia gravis by immunoadsorption of plasma. Neurology 45, 338–344.
Stoffel W and Demant T (1981). Selective removal of apolipoprotein B containing serum lipoproteins from blood plasma. Proc Natl Acad Sci 78, 611–615.
Stoffel W, Borberg H, Greve V (1981). Application of specific extracorporeal removal of low density lipoprotein in familial hypercholesterolemia. Lancet ii, 1005–1007.
Müller-Derlich J, du Moulin A, Spaethe R (1993). Specific removal of human immunoglobulin with IgGTherasorb. Artif Organs 17, 546.
Leventhal JR, John R, Fryer JP, Witson JC, Müller-Derlich J, Remiszewski J, Dalmasso AP, Matas AJ, Bol-man RM (1995). Removal of baboon and human antiporcine IgG and IgM natural antibodies by immunoadsorption. Transplantation 59, 294–300.
Dinarello CA (1991). Interleukin-1 and inlerleukin-1 antagonism. Blood 77, 1627–1652.
Dinarello CA, Cannon JG, Wolff SM, Bernheim HA, Beutler B, Cerami A, Figari IS, Palladino MA, O’Connor JV (1986). Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med 163, 1433–1450.
Casey LC, Balk RA, Bone RC (1993). Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome. Ann Intern Med 119, 771–778.
Falkenhagen D (1995). Small particles in medicine. Artif Organs 19, 792–794.
Weber C, Rajnoch C, Loth F, Schima H, Falkenhagen D (1994). The Microspheres based Detoxification System (MDS). A new extracorporeal blood purification technology based on recirculated microspherical adsorbent particles. Int J Artif Organs 17, 595–602.
von Appen K, Weber C, Losert U, Schima H, Gurland HJ, Falkenhagen D (1996). Microspheres based Detoxification System: a new method in convective blood purification. Artif Organs 20, 420–425.
Weber C, Henne B, Loth F, Schoenhofen M, Falkenhagen (1995). Development of cationically modified cellulose adsorbents for the removal of endotoxins. ASAIO J 41, M430 - M434.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media New York
About this chapter
Cite this chapter
Weber, C., Falkenhagen, D. (1997). Specific Blood Purification by Means of Antibody-Conjugated Magnetic Microspheres. In: Häfeli, U., Schütt, W., Teller, J., Zborowski, M. (eds) Scientific and Clinical Applications of Magnetic Carriers. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-6482-6_28
Download citation
DOI: https://doi.org/10.1007/978-1-4757-6482-6_28
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4419-3283-9
Online ISBN: 978-1-4757-6482-6
eBook Packages: Springer Book Archive