Abstract
Since its introduction in 1976 (Cullheim and Kellerth, 1976; Jankowska et al., 1976; Kitai et al., 1976; Light and Durkovic, 1976; Snow et al., 1976), the method of intracellular injection of horseradish peroxidase (HRP) has established itself as an enormously productive tool for neurobiology (Kitai and Bishop, 1981; Kitai and Wilson, 1982). The fundamental advantage of the technique is that it allows direct correspondence between cellular physiology and morphology to be established. First, as a physiological tool, the HRP-filled microelectrode is suitable for the analysis of any neurophysiological property of a neuron that can be assayed by intracellular recording. Second, as a morphological tool, intracellular iontophoresis of HRP fills and labels the entire extent of a neuron, including soma, dendrites, dendritic specializations such as spines, and as much of the axon, axonal collaterals, and terminals as survival time permits. The morphological rendition of the HRP-filled neuron revealed by enzyme histochemistry is equal to or better than the results of the very best Golgi stains.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adams, J. C. 1977, Technical considerations on the use of horseradish peroxidase as a neuronal marker, Neuroscience 2:141–145.
Afsharpour, S., Kita, H., Penny, G. R., and Kitai, S. T., 1985, Glutamate acid decarboxylase, substance P and Leu-enkephalin-immunoreactive neurons in the neostriatum that project to the globus pallidus and substantia nigra, Soc. Neurosci. Abstr. 11:362.
Bargas, J., Galarraga, E., Chang, H. T., and Kitai, S. T., 1988, Electrophysiological and double-labeling immunohistochemical analyses of neurons in the substantia nigra zona compacta of the rat, Soc. Neurosci. Abstr. 14:1025.
Berod, A., Hartman, B. K., and Pujol, J. F., 1981, Importance of fixaton in immunocytochem-istry: Use of formaldehyde solutions at variable pH for the localization of tysosine hydroxylase, J. Histochem. Cytochem. 29:844–850.
Brown, K. T., and Flaming, D. G., 1986, Advanced Micropipette Techniques for Cell Physiology, John Wiley & Sons, New York.
Brown, P. G., Maxfield, B. W., and Moraff, H., 1973, Electronics for Neurobiologists, MIT Press, Cambridge, MA.
Chang, H. T., 1988, Dopamine-acetylcholine interaction in the striatum: A dual-labeling im-munocytochemical study of tyrosine hydroxylase and choline acetyltransferase positive elements in the rat, Brain Res. Bull. 21:295–304.
Chang, H. T., Waters, R. S., and Kitai, S. T., 1986, Intracellular labeling combined with im-munocytochemistry in the rat substantia nigra, Soc. Neurosci. Abstr. 12:654.
Colman, D. R., Scalia, F., and Cabrales, E., 1976, Light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat, Brain Res. 102:156–163.
Culheim, S., and Kellerth, J. O., 1976, Combined light and electron microscopic tracing of neurons including axons and synaptic terminals after the intracellular injection of horseradish peroxidase, Neurosci. Lett. 2:307–313.
Czerkensky, C. C., Nilsson, L. A., Nygran, H., Ouchterlony, O., and Tarkowski, A., 1983, A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secretion cells, J. Immunol. Methods 65:109–121.
Dingledine, R. (ed.), 1984, Brain Slices, Plenum Press, New York.
Donoghue, J. P., and Herkenham, M., 1983, Multiple patterns of corticostriatal projections and their relationship to opiate receptor patches in rats, Soc. Neurosci. Abstr. 9:15.
Gerfen, C. R., 1984, The neostriatal mosaic: Compartmentalization of corticostriatal input and striatonigral output systems, Nature 314:461–464.
Gerfen, C. R., 1985, The neostriatal mosaic: I. Compartmental organization of projections from the striatum to the substantia nigra in the rat, J. Comp. Neurol. 236:454–476.
Goldman, P. S., and Nauta, W.J. H., 1977, An intracately patterned prefronto-caudate projection in the rhesus monkey, J. Comp. Neurol. 171:369–386.
Goldman-Rakic, P. S., 1982, Cytoarchitechtectonic heterogenity of the primate neostriatum: Subdivisions into island and matrix cellular compartments, J. Comp. Neurol. 205:398–413.
Graybiel, A. M., 1983, Compartmental organization of the mammalian striatum, in: Progress in Brain Research, Volume 58: Molecular and Cellular Interactions Underlying Higher Brain Functions (J. P. Changeux, J. Glowinski, M. Imbert, and F. E. Bloom, eds.), Elsevier, New York, pp. 247–256.
Graybiel, A. M., and Ragsdale, C. W., 1978a, Histochemically distinct compartments in the striatum of human, monkey, and cat demonstrated by acetylthiocholinesterase staining, Proc. Natl. Acad. Sci. U.S.A. 75:5723–5726.
Graybiel, A. M., and Ragsdale, C. W., 1978b, Striosomal organization of the caudate nucleus. I. Acetylcholinesterase histochemistry of the striatum in the cat, rhesus monkey, and human being, Soc. Neurosci. Abstr. 4:44.
Graybiel, A. M., Ragsdale, C. W., and Edley, S. M., 1979, Compartments in the striatum of the cat observed by retrograde cell labeling, Exp. Brain Res. 34:189–195.
Graybiel, A. M., Ragsdale, C. W., Yoneka, E. S., and Elde, R. P., 1981, An immunohistochem-ical study of enkephalins and other neuropeptides in the striatum of the cat with evidence that the opiate peptides are arranged to form mosaic patterns to register with the striosomal compartments visible by acetylcholinesterase staining, Neuroscience 6:377–382.
Herkenham, M., and Pert, C. B., 1981, Mosaic distribution of opiate receptors, parafascicular projections, and acetylcholinesterase in rat striatum, Nature 291:415–418.
Herkenham, M., Edley, S. M., and Stuart, J., 1984, Cell clusters in the nucleus accumbens of the rat and the mosaic relationship of the opiate receptors, acetylcholinesterase and subcortical afferent termination, Neuroscience 11:561–591.
Holgate, C. S., Jackson, P., Cowen, P. N., and Bird, C. C., 1983, Immunogold-silver staining: New method of immunostaining with enhanced sensitivity, J. Histochem. Cytochem. 31:938–944.
Horikawa, K., and Armstrong, W. E., 1988, A versatile means of intracellular labeling: Injection of biocytin and its detection with avidin conjugates, J. Neurosci. Methods 25:1–11.
Hsu, S. M., Raine, L., and Ganger, H., 1981, The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures, J. Histochem. Cytochem. 29:577–580.
Itoh, K., Konishi, A. Nomura, S., Mizuno, N., Nakamura, Y., and Sugimoto, I., 1979, Application of coupled oxidation reaction to electron microscope demonstration of horseradish peroxidase: Cobalt-glucose oxidase method, Brain Res. 175:341–346.
Jankowska, E., Rastad, R., and Westman, J., 1976, Intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spino-cervical tract cells, Brain Res. 105:555–562.
Kalil, K., 1978, Patch-like termination of thalamic fibers in the putamen of the rhesus monkey: An autoradiographic study, Brain Res. 140:333–339.
Kitai, S. T., and Bishop, G. A., 1981, Intracellular staining of neurons, in Neuroanatomical Tract-Tracing Methods (L. Heimer and M. J. RoBards, eds.), Plenum Press, New York, pp. 263–277.
Kitai, S. T., and Kita, H., 1984, Electrophysiological study of the neostriatum in brain slice preparation, in: Brain Slices (R. Dingledine, ed.), Plenum Press, New York, pp. 285–296.
Kitai, S. T., and Wilson, C. J., 1982, Intracellular labeling of neurons in mammalian brains, in: Cytochemical Methods in Neuroanatomy (V. Chan-Palay and S. Palay, eds.), Alan R. Liss, New York, pp. 533–549.
Kitai, S. T., Kosis, J. D., Preston, R. J., and Sugimori, M., 1976, Monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase, Brain Res. 109:601–606.
Light, A. R., and Durkovic, R. G., 1976, Horseradish peroxidase: An improvement in intracellular straining of single electrophysiologically characterized neurons, Exp. Neurol. 53:847–853.
Lighthall, J. W., Park, M. R., and Kitai, S. T., 1981, Inhibition in slices of rat neostriatum, Brain Res. 212:182–187.
McLean, I. W., and Nakane, P. K., 1974, Periodate-lysine-paraformaldehyde fixative: A new fixative for immunoelectron microscopy, J. Histochem. Cytochem. 22:1077–1083.
Misgeld, U., and Bak, I. J., 1979, Intrinsic excitation in the rat neostriatum mediated by acetylcholine, Neurosci. Lett. 12:277–282.
Morrison, R., 1986, Grounding and Shielding Techniques in Instrumentation, John Wiley & Sons, New York.
Nobin, A., and Bjorklund, A., 1973, Topography of the monoamine neuron system in the human brain as revealed in fetuses, Acta Physiol. Scand. 88:1–40.
Olson, L., Boreus, L., and Seiger, A., 1972a, Histochemical demonstration and mapping of 5-hydroxytryptamine and catecholamine-containing neuron systems in the human fetal brain, Z. Anat. Entwickl. Gesch. 139:259–282.
Olson, L., Seiger, A., and Fuxe, K., 1972b, Heterogenity of striatal and limbic dopamine innervation: Highly fluorescent islands in developing and adult rats, Brain Res. 44:283–288.
Park, M. R., 1985, A complete digitally neurophysiological recording laboratory, in: The Microcomputer in Cell and Neurobiology Research (R. R. Mize, ed.), Elsevier, New York, pp. 411–434.
Penny, G. R., Wilson, C. J., and Kitai, S. T., 1984, The influence of neostriatal patch and matrix compartments on the dendritic geometry of spiny projection neurons in the rat as revealed by intracellular labeling with HRP combined with immunocytochemistry, Soc. Neurosci. Abstr. 10:514.
Penny, G. R., Chang, H. T., and Kitai, S. T., 1986, Dual localization of [Leu]enkephalin and choline acetyltransferase in the rat basal ganglia, Soc. Neurosci. Abstr. 12:1328.
Penny, G. R., Wilson, C. J., and Kitai, S. T., 1988, Relationship of the axonal and dendritic geometry of spiny projection neurons to the compartmental organization of the neostriatum, J. Comp. Neurol. 269:275–289.
Purves, R. D., 1981, Microelectrode Methods for Intracellular Recording and Ionophoresis, Academic Press, London.
Ragsdale, C. W., and Graybiel, A. M., 1984, Further observations on the striosomal organization of frontostriatal projections in cats and monkeys, Soc. Neurosci. Abstr. 10:514.
Sandell, J. H., and Masland, R. H., 1988, Photoconversion of some fluorescent markers to a diaminobenzidine product, J. Histochem. Cytochem. 36:555–559.
Scholer, J., and Armstrong, W. K., 1982, Aqueous aldehyde (FAGLU) histofluorescence for catecholamines in 2 μm sections using polyethylene glycol embedding, Brain Res. Bull. 9:27–31.
Snow, P. J., Rose, P. K., and Brown, A. G., 1976, Tracing axons and axon collaterals of spinal neurons using intracellular injections of horseradish peroxidase, Science 191:312–313.
Somogyi, P., and Soltesz, I., 1986, Immunogold demonstration of GABA in synaptic terminals of intracellularly recorded, horseradish peroxidase-filled basket cells and clutch cells in the cat’s visual cortex, Neuroscience 19:1051–1065.
Sternberger, C. A., 1986, Immunocytochemistry, 3rd ed., John Wiley & Sons, New York.
Tennyson, V. M., Barrett, R. E., Cohen, G., Cote, L., Heikkila, R., and Mytineou, C., 1972, The developing neostriatum of the rabbit: Correlation of fluoresence histochemistry electron microscopy, endogenous dopamine levels, and [3H]dopamine uptake, Brain Res. 46:2541–285.
van den Pol, A. N., 1984, Colloidal gold and biotin-avidin conjugates as ultrastructural markers for neural antigens, Q. J. Exp. Physiol. 69:1–33.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kitai, S.T., Penny, G.R., Chang, H.T. (1989). Intracellular Labeling and Immunocytochemistry. In: Heimer, L., Záborszky, L. (eds) Neuroanatomical Tract-Tracing Methods 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-2055-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-2055-6_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-2057-0
Online ISBN: 978-1-4757-2055-6
eBook Packages: Springer Book Archive