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Components of the Kallikrein-Kinin System in Urine

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Kinins—II

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 120))

Abstract

The excretion of kallikrein in urine varies, but the pathophysiologic implications are not clear. To help clarify the role of the urinary kallikrein-kinin system, we have begun to define components of the system as they occur in urine. To minimize artifacts which may arise through extensive purification procedures, we studied urinary protein concentrates prepared by ultrafiltration. The concentrates were separated by chromatography on Sephacryl. Urine contains abundant kininase activity, but in strongly inhibited forms. Kininase II is separable into at least two forms. Another major kininase can hydrolyze benzoyl-Pro-Phe-Arg and is inhibited by arginine but not by BPP9a or SQ 14,225. Its molecular weight is ~63,000. A third kininase, not inhibited by BPP9a, is excluded from Sephacryl. Human urine appears to contain only one kallikrein-like enzyme (MM 45,000). In addition, urine conta:ins a protein (MW ~80,000) which reacts with trypsin to release bradykinin and which inhibits the hydrolysis of Pro-Phe-Arg-[3H]anilide by urinary kallikrein. Thus, in addition to kallikrein and kinins, urine contains kininogen and at least three kininase enzymes. Urinary ultrafiltrate contains an inhibitory substance (~MW 400).

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Author information

Authors and Affiliations

  1. Department of Medicine, University of Miami School Medicine, Miami, Florida, 33101, USA

    James W. Ryan, Narendra B. Oza, Larry C. Martin & Guillermo A. Pena

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  1. James W. Ryan
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  2. Narendra B. Oza
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  3. Larry C. Martin
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  4. Guillermo A. Pena
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Editor information

Editors and Affiliations

  1. Institute for Protein Research, Osaka University, Osaka, Japan

    Setsuro Fujii

  2. Science University of Tokyo, Tokyo, Japan

    Hiroshi Moriya

  3. Meiji College of Pharmacy, Tokyo, Japan

    Tomoji Suzuki

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© 1979 Springer Science+Business Media New York

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Ryan, J.W., Oza, N.B., Martin, L.C., Pena, G.A. (1979). Components of the Kallikrein-Kinin System in Urine. In: Fujii, S., Moriya, H., Suzuki, T. (eds) Kinins—II. Advances in Experimental Medicine and Biology, vol 120. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0926-1_30

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  • DOI: https://doi.org/10.1007/978-1-4757-0926-1_30

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4757-0928-5

  • Online ISBN: 978-1-4757-0926-1

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