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Assay of Angiotensin I by Fluorescence Polarization Method

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Kinins—II

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 120))

Abstract

Fluorescence polarization technique was applied for the assay of angiotensin I (Al) in human plasma. In this assay system, fluorescein labeled Al (F-Al), which retained the original antigenicity, and antibody to Al was allowed to interact in a cuvette in the instruments yielding an increase in the fluorescence polarization (P) value. Non-labeled Al in the sample blocked the binding of F-Al to the antibody resulting lower P value. Log of antigen concentration and P value was found to exhibit reverse linear proportionality between 0.05 ng to 2 ng/ml of antigen (Al) concentration. The present method was compared with standard radio-immunoassay method and the result showed that data were compatible with each other. The calculation of P value is automated and three cavity filter and optics of the instrument gave reliable results. The method is fast (<2 min), sensitive (<10 picomole/ml) and simple (no separation step before readout of the results).

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Author information

Authors and Affiliations

  1. Departments of Microbiology, Kumamoto University Medical School, Kumamoto 860, Japan

    Hiroshi Maeda

  2. Internal Medicine, Kumamoto University Medical School, Kumamoto 860, Japan

    Mahito Nakayama, Daisuke Iwaoka & Tatsuo Sato

Authors
  1. Hiroshi Maeda
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  2. Mahito Nakayama
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  3. Daisuke Iwaoka
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  4. Tatsuo Sato
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Editor information

Editors and Affiliations

  1. Institute for Protein Research, Osaka University, Osaka, Japan

    Setsuro Fujii

  2. Science University of Tokyo, Tokyo, Japan

    Hiroshi Moriya

  3. Meiji College of Pharmacy, Tokyo, Japan

    Tomoji Suzuki

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© 1979 Springer Science+Business Media New York

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Maeda, H., Nakayama, M., Iwaoka, D., Sato, T. (1979). Assay of Angiotensin I by Fluorescence Polarization Method. In: Fujii, S., Moriya, H., Suzuki, T. (eds) Kinins—II. Advances in Experimental Medicine and Biology, vol 120. Springer, New York, NY. https://doi.org/10.1007/978-1-4757-0926-1_20

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  • DOI: https://doi.org/10.1007/978-1-4757-0926-1_20

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4757-0928-5

  • Online ISBN: 978-1-4757-0926-1

  • eBook Packages: Springer Book Archive

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