Abstract
In the near-infrared region, two chromophores of biological interest, haemoglobin and cytochrome a,a3, have specific absorption bands, which respond to the oxygenation state of living tissues. Several authors have applied near-infrared spectroscopy to their analysis in situ(e.g. Jobsis, 1977; Gianni et al., 1982; Kariman and Burkhart, 1985a), but quantitative analyses in tissue have encountered difficulties from the overlap of changes in the amount and oxygenation state of haemoglobin and the redox state of cytochrome a,a3.
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References
Chance, B. (1966). Spectrophotometric observations of absorbance changes in the infrared region in suspensions of mitochondria and in submitochondrial particles. In: The Biochemistry of Copper. Eds Peisach, G., Aisen, P. and Blumberg, W.E., Academic Press, New York, pp. 293–301.
Ferrari, M., Gianni, I., Carpi, A. and Fasella, P. (1983). Noninvasive near-infrared spectroscopy of brain in fluorocarbon exchange transfused rats. Physiol. Chem. Phys. Med. NMR, 15, 107–113.
Gianni, L., Ferrari, M., Carpi, A. and Fasella, P. (1982). Rat brain monitoring by near-infrared spectroscopy: an assessment of possible clinical significance. Physiol. Chem. Phys. 14, 295–305.
Griffith, D.E. and Wharton, D.C. (1961). Studies of the electron transport system. XXXV. Purification and properties of cytochrome oxidase. J. Biol. Chem. 236, 1850–1856.
Jobsis, F.F. (1977). Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science, 198, 1264–1267.
Kariman, K. and Burkhart, D.S. (1985a). Heme-copper relationship of cytochrome oxidase in rat brain in situ. Biochem. Biophys. Res. Commun. 126, 1022–1028.
Kariman, K. and Burkhart, D.S. (1985b). Non-invasive in vivo spectrophotometric monitoring of brain cytochrome aa3 revisited. Brain Res. 360, 203–213.
Seiyama, A., Hazeki, O. and Tamura, M. (1987). Simultaneous measurement of haemoglobin oxygenation of brain and skeletal muscle of rat in vivo by near-infrared spectrophotometry. This volume.
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© 1987 Plenum Press, New York
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Hazeki, O., Seiyama, A., Tamura, M. (1987). Near-Infrared Spectrophotometric Monitoring of Haemoglobin and Cytochrome a,a3 in Situ . In: Silver, I.A., Silver, A. (eds) Oxygen Transport to Tissue IX. Advances in Experimental Medicine and Biology, vol 215. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7433-6_31
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DOI: https://doi.org/10.1007/978-1-4684-7433-6_31
Publisher Name: Springer, Boston, MA
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