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Molecular Biology of Hemopoiesis pp 289–301Cite as

Molecularly Characterized Factors Governing the Growth of Murine Multipotent Stem Cells in Serum-Depleted Marrow Cultures

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 34))

Abstract

The growth requirements of normal murine marrow-derived multipotent stem cells (CFU-GEMM) in a simple clonal cell culture system substantially devoid of exogenous serum proteins was assessed. The ability of murine interleukin-3 (I1–3), recombinant human erythropoietin (rEPO), and a crystalline preparation of the protoporphyrin hemin to support colony growth in “serum-free” cultures was examined by titration. The results suggest that both I1–3 and hemin are limiting for multipotential colony growth in “serum-free” cultures, but that EPO is not. In addition, the ‘sensitivity’ of CFU-GEMM to each growth factor appeared to increase in the “serum-free” environment as evidenced by a “shift-to-the-left” in all the titration curves. Nearly half of the GEMM colonies grew to full maturity in the absence of exogenous EPO. Given the optimal concentration of each growth factor, high colony growth was consistently observed in the “serum-free” cultures, with a range from 65% to 119% of the serum control level. It is therefore concluded that supplementation of murine marrow cultures with I1–3 and hemin alone may provide the necessary setting for studying the factors which modulate the growth of multipotent stem cells in a serum- free environment.

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Authors and Affiliations

  1. Department of Biology, Boston University, Boston, MA, 02215, USA

    Francis C. Monette & George Siqounas

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  1. Francis C. Monette
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  2. George Siqounas
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Editors and Affiliations

  1. Veterans Administration Medical Center, University of Mississippi, Jackson, Mississippi, USA

    Mehdi Tavassoli

  2. Veterans Administration Medical Center, University of Nevada, Reno, Nevada, USA

    Esmail D. Zanjani

  3. New York Medical College, Valhalla, New York, USA

    Joao L. Ascensao  & Nader G. Abraham  & 

  4. Division of Blood Diseases and Resources, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA

    Alan S. Levine

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© 1988 Plenum Press, New York

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Monette, F.C., Siqounas, G. (1988). Molecularly Characterized Factors Governing the Growth of Murine Multipotent Stem Cells in Serum-Depleted Marrow Cultures. In: Tavassoli, M., Zanjani, E.D., Ascensao, J.L., Abraham, N.G., Levine, A.S. (eds) Molecular Biology of Hemopoiesis. Advances in Experimental Medicine and Biology, vol 34. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5571-7_32

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  • DOI: https://doi.org/10.1007/978-1-4684-5571-7_32

  • Publisher Name: Springer, Boston, MA

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