Abstract
Oxygen transfer in blood is receiving increasing attention because of current activities in the areas of heart transplants and oxygenator development. In the course of our work on simultaneous diffusion and chemical reaction (1) of oxygen within red cells, it became necessary to measure the concentration of uncombined oxygen in intact cells. Since no method was available, a technique using polarographic PO2 electrodes was developed and is described in this paper. The data thus obtained was used in the governing equations for intracellular oxygen transport to evaluate the overall diffusion coefficient of oxygen in the red cell at 25°C.
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References
Fleischman,M.,“Simultaneous Diffusion and Chemical Reaction of Oxygen in Whole Blood”, Ph.D. Thesis, Univ. of Cincinnati, 1968
Keller, K.H., “The Steady State Transport of Oxygen thru Hemoglobin Solutions”, Ph.D. thesis, Johns Hopkins Univ., 1965
Longmuir, I.S., Roughton, F.J.W., “The Diffusion Coefficients of CO and N2 in Hemoglobin Solutions”, J. Physiol. 118, 264 (1952)
Bird, ROB., Stewart, W.E., & Lightfoot, E.N., “Transport Phenomena”, p. 545, John Wiley (1960)
Kreuzer, F., Yahr, W.Z., “Influence of Red Cell Membrane in Diffusion of Oxygen”, J. App. Physiol. 15, 1123–29 (1960)
Sirs, J.A., “Uptake of 02 and CO by Hemoglobin in Sheep Erythrocytes at Various Temperatures”, J. App. Physiol. 18, 166–170 (1963)
Mochizuki, M., Fukuoka, J.I., “The Diffusion of Oxygen Inside the Red Cell”, Japan J. Physiol. 8, 206–224 (1958)
Sendroy, J., Van Slyke, D.D., “Solubility and Physical State of Uncombined Oxygen in Blood”, J. Biol. Chem. 105, 597–632 (1954)
Peters, J.P., Van Slyke, D.D., “Quantitative Clinical Chemistry”, Vol. II, Williams & Wilkins, Bait. (1932)
Hershey, D. and Karhan, T,L „ “Equilibrium and Diffusion of the 02 Blood System,” A.I.Ch. E. Journal, 14, 969–973 (1968)
Kelman, G,R., “Correction Factors to be applied to Measured Blood Gas Tensions and pH”, Bio. Med. Eng 8/67, pp 362–64
Handbook of Chemistry and Physics, 39th ed., Chem. Rubber Co., p. 1606, 1957–1958
Crank, J., “The Mathematics of Diffusion”, p. 87, Oxford Press, 1956
Lapidus, L., “Digital Computation for Chemical Engineers”, McGraw-Hill, 1962
Fasciolo, J.C., Chiodi, H., “Arterial P02 During Pure 02 Breathing”, Am. J. Physiol. 147 54–65 (1946)
Sirs, J.A., “Influence of Metabolism on Uptake of CO and O2 by Hemoglobin in Erythrocytes“, J. Appl. Physiol. 18, 175–178 (1963)
Whyte, J.H., Laver, M.B., “Oxygen Solubility in Blood and Temperature Corrections for P02”, J. Appl. Physiol. 19, 901–906 (1963)
Hesselberth, J.F., “Simultaneous Diffusion and Chemical Reaction of Oxygen in Whole Blood in a Wetted Wall Column”, Ph.D. thesis, Univ. of Cincinnati, 1968
Perutz, M.F., “Submicroscopic Structure of the Red Cell”, Nature 161, 2O- (19+8)
Fleischman, M., Hershey, D., “Estimation of the Rate Constants for the Oxygen-Hemoglobin Reaction Within Intact Red Cells”, In Press, CEP Symposium Series, AIChE
Anderson, N.M., Sekelj, P., “The Light Transmission of Whole Blood-Determination of Oxygen Saturation”, J. Lab. Clin. Med. 65, 153–166 (1965)
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© 1970 Plenum Press, New York
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Fleischman, M., Hershey, D. (1970). In Vitro Measurement of Uncombined Oxygen Concentration in Intact Red Cells. In: Hershey, D. (eds) Blood Oxygenation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1857-6_5
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DOI: https://doi.org/10.1007/978-1-4684-1857-6_5
Publisher Name: Springer, Boston, MA
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