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In Vitro Measurement of Uncombined Oxygen Concentration in Intact Red Cells

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Blood Oxygenation

Abstract

Oxygen transfer in blood is receiving increasing attention because of current activities in the areas of heart transplants and oxygenator development. In the course of our work on simultaneous diffusion and chemical reaction (1) of oxygen within red cells, it became necessary to measure the concentration of uncombined oxygen in intact cells. Since no method was available, a technique using polarographic PO2 electrodes was developed and is described in this paper. The data thus obtained was used in the governing equations for intracellular oxygen transport to evaluate the overall diffusion coefficient of oxygen in the red cell at 25°C.

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© 1970 Plenum Press, New York

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Fleischman, M., Hershey, D. (1970). In Vitro Measurement of Uncombined Oxygen Concentration in Intact Red Cells. In: Hershey, D. (eds) Blood Oxygenation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1857-6_5

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  • DOI: https://doi.org/10.1007/978-1-4684-1857-6_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-1859-0

  • Online ISBN: 978-1-4684-1857-6

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