Turnover of Molecular Species of Diacyl and Alkyl Ether Phospholipids in Ehrlich Ascites Tumor Cells

Abstract

The biosynthetic route of alkyl ether phospholipid has been established by Hajra (1,2) and Snyder (3), and the biological role of this phospholipid has been studied by several researchers (4–7). A significant amount of 1-0-alkyl-2-acyl-GPE(C) was found in Ehrlich ascites tumor cells (8) and the rapid turnover rate of ethanolamine alkyl ether phospholipid has been demonstrated by an incorporation experiment of phospholipid precursors, ³²P_i, [l-¹⁴C]-glycerol and [1-¹⁴C]acetate injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells (9). In this paper, [1-¹⁴C]glycerol was injected into the peritoneal cavity of mice bearing Ehrlich ascites tumor cells and the incorporation rate of radioactivity into every molecular species of alkyl ether phospholipids was estimated.