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Cytochromes P-450 and b5 pp 467–483Cite as

The Role of Cytochrome b5 in Mixed Function Oxidations: Effect of Microsomal Binding of the Hemoprotein on Hepatic N-Demethylations

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 58))

Summary

Incubation of rat cytochrome b5 (D-b5) with rat liver microsomes resulted in specific binding of the hemoprotein. The bound hemoprotein was rapidly reduced by NADH. The NADH cytochrome c reductase activity in these preparations increased in proportion to the amount of cytochrome. In contrast to D-b5, which inhibited N-demethylation and the NADH synergism, the binding of cytochrome b5 preparations, reconstituted from heme and apocyto-chrome b5 had no effect on either the NADPH-dependent N-demethylation of aminopyrine or ethylmorphine or the NADH synergism observed with rat liver microsomes. In addition, manganese protoporphyrin-apocytochrome complex, when bound to microsomes in amounts equivalent to D-b5, showed no effect on N-demethylation activity. These results suggest that homogeneous cytochrome b5 contains contaminating amounts of tightly bound detergent which presumably is removed during the extraction of the heme from the apocytochrome.

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Authors and Affiliations

  1. Departments of Pharmacology and Biochemistry, Health Center, University of Connecticut, Farmington, Connecticut, 06032, USA

    Dominick L. Cinti & Juris Ozols

Authors
  1. Dominick L. Cinti
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  2. Juris Ozols
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Editors and Affiliations

  1. Harrison Department of Surgical Research, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

    David Y. Cooper  & Otto Rosenthal  & 

  2. Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Robert Snyder  & Charlotte Witmer  & 

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© 1975 Plenum Press, New York

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Cinti, D.L., Ozols, J. (1975). The Role of Cytochrome b5 in Mixed Function Oxidations: Effect of Microsomal Binding of the Hemoprotein on Hepatic N-Demethylations. In: Cooper, D.Y., Rosenthal, O., Snyder, R., Witmer, C. (eds) Cytochromes P-450 and b5 . Advances in Experimental Medicine and Biology, vol 58. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9026-2_32

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  • DOI: https://doi.org/10.1007/978-1-4615-9026-2_32

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9028-6

  • Online ISBN: 978-1-4615-9026-2

  • eBook Packages: Springer Book Archive

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