Abstract
We have cloned, sequenced and expressed a recombinant group IX pollen allergen from barley (Hordeum vulgare). Hor v 9 is a polypeptide of 313 amino acids. The Hor v 9 cDNA clone was engineered into the E. coli protein expression vector pMAL and expressed as a fusion of maltose binding protein and truncated Hor v 9. Polyclonal antibodies to the fusion protein were raised in mice. Cross-reactive proteins, RNA and DNA homologues were found in many agricultural species including wheat, rye, triticale, oats, maize, sunflower and flax. The presence of group IX-like proteins in a variety of agricultural crops may represent a previously uncharacterized aeroallergenic occupational hazard. Sequence comparisons of the barley allergen, Hor v 9, with Poa p 9 and other cloned group IX pollen allergens revealed putative structural domains common to all. These include a signal peptide, two conserved immunoglobulin-like motifs, a 150 amino acid highly conserved carboxyterminal domain and a carboxyterminal transmembrane helix. This structural arrangement is also found in cell adhesion molecules. The highly conserved T-cell epitope previously characterized and mapped in group IX allergens (and present in Hor v 9) was found in several human cell adhesion molecule sequences (VCAM, NCAM and CD2). This T-cell epitope corresponded to the most highly conserved amino acid residues common to all group IX homologues sequenced to date. CD2 and VCAM are known to play a role in allergic inflammation: VCAM is involved in the recruitment of lymphocytes to sites of inflammation, and cross-linking CD2 leads to T-cell activation. .We anticipate that the similar structural arrangement of group IX allergens and human cell adhesion molecules, as well as the presence of a T-cell epitope common to group IX pollen allergens and cell adhesion molecules, will have important consequences in the natural history of the atopic immune response.
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References
Silvanovich, A., Astwood, I, Zhang, L, Olsen, E., Kisil, F., Sehon, A., Mohapatra S. and Hill, R. (1991) Nucleotide sequence analysis of three cDNAs coding for Poap IX isoallergens of kentucky bluegrass pollen. J. Biol. Chem. 266, 1204–1210.
Ong, E.K., Griffith, I.J., Knox, R.B. and Singh, M.B. (1993) Cloning of a cDNA encoding a group-V (group IX) allergen isoform from rye-grass pollen that demonstrates specific immunoreactivity. Gene 134, 235–240.
Vrtala, S., Sperr, W.R., Reimitzer, I., van Ree, R., Laffer, S., Muller, W-D., Valent, P., Lechner, K., Rum-pold, H., Kraft, D., Scheiner, O., and Valenta, R. (1993) cDNA cloning of a major allergen from Timothy grass (Phleum pratense) pollen, characterization of the recombinant Phl p V allergen. J. Immunol. 151, 4773–4781.
Astwood, J.D., Mohapatra, S.S., Ni, H. and R.D. (1994) Pollen allergen homologues in barley and other crop species. Clin. Exp. Allergy 25, 66–72.
Zhang, L., Olsen, E., Kisil, F.T., Hill, R.D., Sehon, A.H., and Mohapatra, S.S (1992) Mapping of antibody binding epitopes of a recombinant Poap IX allergen. Mol. Immunol. 29, 1383–1389.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular cloning: a laboratory manual, second edition. Cold Spring Harbour: Cold Spring Harbour Laboratory Press.
Mohapatra, S.S., Hill, R., Astwood, J., Ekramoddoullah, A.K.M., Olsen, E., Silvanovich, A., Hatton, T., Kisil, F.T. and A.H. (1990) Isolation and characterization of a cDNA clone encoding an IgE-binding protein from kentucky bluegrass (Poa pratensis) pollen. Int. Arch. Allergy Appl. Immunol. 91, 362–368.
von Heijne, G. (1985) Signal sequences, the limits of variation. J. Mol. Biol 184, 99–105.
Hunt, L.T. and M.O. (1970) The occurrence in proteins of the tripeptides Asn-X-Ser and Asn-X-Thr and of bound carbohydrate. Biochem. Biophys. Res. Commun. 39,757–765.
Harlow, E and Lane, P. (1988) Antibodies, A Laboratory Manual. Cold Spring Harbour: Cold Spring Harbour Laboratory Press.
Singh, M.B., Hough, T., Theerakulpisut, P., Avjioglu, A., Davies, S., Smith, P.M., Taylor, P., Simpson, R.J., Ward, L.D., McCluskey, J., Puy, R. and Knox, R.B. (1991) Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast. Proc. Natl. A cad. Sci. USA 88, 1384–1388.
Pearson, W.R. and D.J. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85, 2444–2448.
Deveraux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nuc. Acids Res. 12, 387–395.
Georjon, C, Deleage, G. and Roux, B. (1991) ANTHEPRO: an interactive graphic software for analyzing protein structures from sequences. J. Mol. Graph. 9, 188–190.
Williams, A.F. and Barclay, A.N. (1988) The immunoglobulin superfamily-domains for cell surface recognition. Ann. Rev. Immunol. 6, 381–405.
Obrink, B. (1993) Cell adhesion and the cell-cell contact proteins. In Guidebook to the extracellular matrix and adhesion proteins. (Kreis, T and Vale, R., eds). Oxford: Oxford University Press, pp. 109–114.
Canonica, G.W., Ciprandi, G., Buscaglia, S., Pesce, G. and Bagnasco, M. (1994) Adhesion molecules of allergic inflammation: recent insights into their functional roles. Allergy 49, 135–141.
Valenta, R., Duchene, M., Pettenburger, K., Sillaber, C, Valent P., Breitenbach, M., Rumpold, H., Kraft, D. and Scheiner, O. (1991) Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals. Science 253, 557–560.
Mohapatra, S.S., Mohapatra, S., Yang, M., Ansari, A.A., Parronchi, P., Maggi, E., and Romagnani, S. (1994) Molecular basis of cross-reactivity among allergen-specific human T cells: T-cell receptor Va gene usage and epitope structure. Immunology 81, 15–20.
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Astwood, J.D., Hill, R.D. (1996). Molecular Characterization Of Hor v 9. In: Sehon, A., HayGlass, K.T., Kraft, D. (eds) New Horizons in Allergy Immunotherapy. Advances in Experimental Medicine and Biology, vol 409. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5855-2_39
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DOI: https://doi.org/10.1007/978-1-4615-5855-2_39
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