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Recombinant Expression and Epitope Mapping of Grass Pollen Allergens

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New Horizons in Allergy Immunotherapy

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 409))

Summary

We have studied the expression of recombinant forms of Group 1 allergens from rye-grass and Bermuda grass pollens. Recombinant Lol p 1 expressed in bacteria bound serum IgE from allergic patients. Based on analysis of fragments of the Lol p 1 cDNA clone, the major IgE-reactive epitope has been mapped to the C-terminus. However, although SDS-denatured natural Cyn d 1 (from Bermuda grass) bound IgE, the full or partial recombinant proteins expressed in bacteria did not bind IgE. We have since expressed Cyn d 1 in the yeast Pichia pastoris and restored IgE binding.

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Authors and Affiliations

  1. School of Botany, The University of Melbourne, Parkville, Vic. 3052, Australia

    Cenk Suphioglu, Penelope M. Smith, Eng K. Ong, R. Bruce Knox & Mohan B. Singh

Authors
  1. Cenk Suphioglu
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  2. Penelope M. Smith
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  3. Eng K. Ong
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  4. R. Bruce Knox
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  5. Mohan B. Singh
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Editor information

Editors and Affiliations

  1. University of Manitoba, Winnipeg, Manitoba, Canada

    Alec Sehon  & Kent T. HayGlass  & 

  2. Institute of General and Experimental Pathology, Vienna, Austria

    Dietrich Kraft

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© 1996 Springer Science+Business Media New York

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Suphioglu, C., Smith, P.M., Ong, E.K., Knox, R.B., Singh, M.B. (1996). Recombinant Expression and Epitope Mapping of Grass Pollen Allergens. In: Sehon, A., HayGlass, K.T., Kraft, D. (eds) New Horizons in Allergy Immunotherapy. Advances in Experimental Medicine and Biology, vol 409. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5855-2_20

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  • DOI: https://doi.org/10.1007/978-1-4615-5855-2_20

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7684-2

  • Online ISBN: 978-1-4615-5855-2

  • eBook Packages: Springer Book Archive

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