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Epigenetic Reprogramming of Mesenchymal Stem Cells

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Epigenetic Alterations in Oncogenesis

Part of the book series: Advances in Experimental Medicine and Biology ((volume 754))

Abstract

Mesenchymal stem cells (MSCs) are multipotent stem cells of mesodermal origin that can be isolated from various sources and induced into different cell types. Although MSCs possess immune privilege and are more easily obtained than embryonic stem cells, their propensity to tumorigenesis has not been fully explored. Epigenomic changes in DNA methylation and chromatin structure have been hypothesized to be critical in the determination of lineage-specific differentiation and tumorigenesis of MSCs, but this has not been formally proven. We applied a targeted DNA methylation method to methylate a Polycomb group protein-governed gene, Trip10, in MSCs, which accelerated the cell fate determination of MSCs. In addition, targeted methylation of HIC1 and RassF1A, both tumor suppressor genes, transformed MSCs into tumor stem cell-like cells. This new method will allow better control of the differentiation of MSCs and their use in downstream applications.

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Authors and Affiliations

  1. Department of Life Science, National Chung Cheng University, Chia-Yi, 621, Taiwan

    Yu-Wei Leu & Shu-Huei Hsiao

  2. Department of Molecular Medicine and Cancer Therapy and Research Center, University of Texas Health Science Center, San Antonio, TX, 78229, USA

    Tim H.-M. Huang

Authors
  1. Yu-Wei Leu
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  2. Tim H.-M. Huang
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  3. Shu-Huei Hsiao
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Corresponding author

Correspondence to Tim H.-M. Huang .

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Editors and Affiliations

  1. University of Nebraska Medical Center, Eppley Institute for Research in Cancer, 985950 Nebraska Medical Center, Omaha, 68198, Nebraska, USA

    Adam R. Karpf

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Leu, YW., Huang, T.HM., Hsiao, SH. (2013). Epigenetic Reprogramming of Mesenchymal Stem Cells. In: Karpf, A. (eds) Epigenetic Alterations in Oncogenesis. Advances in Experimental Medicine and Biology, vol 754. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9967-2_10

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