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Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization

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Practical Manual of In Vitro Fertilization

Abstract

The mechanism of fertilization after ICSI is markedly different from that during natural fertilization. During normal fertilization process, sperm–oocyte fusion is followed by incorporation in the cytoplasm of a demembranated, “naked” sperm nucleus, which immediately becomes accessible for ooplasmic factors, including thiol-reducing agents. Following ICSI, not a “naked” sperm nucleus but the whole sperm cell, enclosed in its membrane, is exposed to the ooplasm.

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Acknowledgments

To Koichi Kyono, MD, PhD, for his extended activation protocol and to Marzeyeh Tavalaee, MS, for the help in preparation of this manuscript

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Authors and Affiliations

  1. Reproductive Laboratories, Advanced Fertility Center of Texas, Houston, TX, USA

    Dmitri Dozortsev MD, PhD

  2. Royan Institute, Isfahan Fertility and Infertility Center, Isfahan, Iran

    Mohammad Hossein Nasr-Esfahani PhD

Authors
  1. Dmitri Dozortsev MD, PhD
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  2. Mohammad Hossein Nasr-Esfahani PhD
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Corresponding author

Correspondence to Dmitri Dozortsev MD, PhD .

Editor information

Editors and Affiliations

  1. , Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, 30342, Georgia, USA

    Zsolt Peter Nagy

  2. , Sr. Embryologist, Montreal Reproductive Centre, Montreal, H4A 3J3, Québec, Canada

    Alex C. Varghese

  3. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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Dozortsev, D., Nasr-Esfahani, M.H. (2012). Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_34

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  • DOI: https://doi.org/10.1007/978-1-4419-1780-5_34

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4419-1779-9

  • Online ISBN: 978-1-4419-1780-5

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