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Practical Manual of In Vitro Fertilization pp 161–169Cite as

In Vivo Embryo Culture Device

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Abstract

The INVO procedure using the INVOcell device is a simple technique of human assisted reproduction. The fertilization of the oocytes and the early development of the embryos occur in the INVOcell device placed into the vaginal cavity for an in vivo incubation. The vaginal cavity plays the role of the CO2 incubator by supplying the temperature and gas environment needed for the embryo development. Capital equipments found in a complex IVF laboratory are not necessary as gametes and embryos are not stored in the laboratory. The INVO procedure can be performed in a medical office setting. INVO is a proven technology that showed clinical pregnancy rates equivalent to conventional IVF when using a prototype. This chapter presents the preliminary results obtained in combining mild ovarian stimulation with INVO using the new designed INVOcell device. The low cost of the INVO procedure and its large availability allow the treatment of a significant portion of infertile couples in the world who could not access any reproductive technology before. The more natural in vivo conception of the embryos with the participation of the patients has generated more interest in INVO from the infertile population and specialized physicians.

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References

  1. Taymor M, Ranoux C, Gross G. Natural oocyte retrieval with intravaginal fertilization: a simplified approach to in vitro fertilization. Obstet Gynecol. 1992;80:888–91.

    PubMed  CAS  Google Scholar 

  2. Ranoux C, Foulot H, Aubriot FX, Poirot C, Dubuisson JB, Chevallier O, Cardone V. A new in vitro fertilization technique: intravaginal culture. Fertil Steril. 1988;49:654–7.

    PubMed  CAS  Google Scholar 

  3. Frydman R, Ranoux C. INVO: a simple, low cost effective assisted reproductive technology. ESHRE Monographs, Special task force on ‘Developing countries and infertility’. Hum Reprod. 2008;163:85–9.

    Google Scholar 

  4. Fukuda M, Fukuda K, Ranoux C. Unexpected low oxygen tension of intravaginal culture. Hum Reprod. 1996;11:1293–5.

    Article  PubMed  CAS  Google Scholar 

  5. Sterzik K, Rosenbusch B, Sasse V, Wolf A, Beier HM, Lauritzen C. A new variation of in vitro fertilization: intravaginal culture of human oocytes and cleavage stages. Hum Reprod Suppl. 1989;4(8 Suppl):83–6.

    CAS  Google Scholar 

  6. Ranoux C, Seibel MM. New techniques in fertilization: intravaginal culture and microvolume straw. J In Vitro Fert Embryo Transf. 1990;7:6–8.

    Article  PubMed  CAS  Google Scholar 

  7. Freude G, Artner B, Leodolter S. Intravaginal culture—simplification of IVF. Wien Med Wochenschr. 1990;140:498–501.

    PubMed  CAS  Google Scholar 

  8. Wiegerinck MAHM, Moret E, Van Dop PA, Wijnberg M, Beerendonk CDMB. Intra vaginal culture (IVC), the Eindhoven experience. In: Evers JHL, Heineman MJ, editors. Ovulation to implantation. Elsevier Science: BV; 1990. p. 349–51.

    Google Scholar 

  9. Sharma S, Hewitt J. Intravaginal culture for IVF. Bombay Hosp J. 1993;35:155–60.

    Google Scholar 

  10. Batres F, Mahadevan M, Maris M, Miller M, Moutos D. Stimulated cycle and intravaginal culture fertilization in an office setting: a preliminary study. Poster presentation (P-196) at the annual meeting of ASRM, Cincinnati, OH; 1997.

    Google Scholar 

  11. Rongieres-Bertrand C, Olivennes F, Righini C, Fanchin R, et al. Revival of the natural cycles in in-vitro fertilization with the use of a new gonadotropin-releasing hormone antagonist (Cetrorelix): a pilot study with minimal stimulation. Hum Reprod. 1999;14:683–8.

    Article  PubMed  CAS  Google Scholar 

  12. Lee TH, Lin YH, Seow KM, et al. Effectiveness of cetrorelix for the prevention of premature luteinizing hormone surge during controlled ovarian stimulation using letrozole and gonadotropins: a randomized trial. Fertil Steril. 2008;90:113–20.

    Article  PubMed  CAS  Google Scholar 

  13. De Silva M, Reeves JJ. Indomethacin inhibition of ovulation in the cow. J Reprod Fertil. 1985;75:547–9.

    Article  PubMed  Google Scholar 

  14. Nargund G, Waterstone J, Bland J. Cumulative conception and live birth rate in natural (unstimulated) IVF cycles. Hum Reprod. 2001;16:259–62.

    Article  PubMed  CAS  Google Scholar 

  15. Requina A, Herrero J, Landeras J, Navarro E, et al. Use of letrozole in assisted reproduction: a systematic review and meta-analysis. Hum Reprod Update. 2008;14:571–82.

    Article  Google Scholar 

  16. Lucena E, Saa AM, Navarro DE, Lombana C, Moran A. Invo Procedure: minimally invasive IVF as an alternative treatment option for infertile couples. The Scientific World Journal. 2012; (in press).

    Article  PubMed  CAS  Google Scholar 

  17. Quinn P. Use of coculture with cumulus cells in insemination medium in human in vitro fertilization (IVF). J Assist Reprod Genet. 1994;11:270–7.

    Article  PubMed  CAS  Google Scholar 

  18. Carrel DT, Peterson CM, Jones KP, Hatasaka HH, et al. A simplified coculture system using homologous, attached cumulus tissue results in improved human embryo morphology and pregnancy rates during in vitro fertilization. J Assist Reprod Genet. 1999;16:344–9.

    Article  Google Scholar 

  19. Ombelet W, Campo R, Bosmans E, Nijs M. Intrauterine insemination (IUI) as a first-line treatment in developing countries and methodological aspects that might influence IUI success. ESHRE Monographs, Special task force on ‘Developing countries and infertility’. Hum Reprod. 2008;2008(1):64–72. doi:10.1093/den,165:64-72.

    Google Scholar 

  20. Ombelet W, Nargund G. The patient from the developing world. In: Macklon NS, Greer IA, Steegers EAP, editors. Textbook of periconceptional medicine. London.: Informa Healthcare; 2009. p. 261–71.

    Google Scholar 

  21. Ombelet W. False perceptions and common misunderstandings surrounding the subject of infertility in developing countries. Hum Reprod. ESHRE Monographs, Special task force on ‘Developing countries and infertility’. Hum Reprod. 2008;204:8–11.

    Google Scholar 

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Author information

Authors and Affiliations

  1. INVO Bioscience, 100 Cummings Center, Suite 207P, Beverly, MA, 01915, USA

    Claude Ranoux MD, MS

Authors
  1. Claude Ranoux MD, MS
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Correspondence to Claude Ranoux MD, MS .

Editor information

Editors and Affiliations

  1. , Scientific and Laboratory Director, Reproductive Biology Associates, Atlanta, 30342, Georgia, USA

    Zsolt Peter Nagy

  2. , Sr. Embryologist, Montreal Reproductive Centre, Montreal, H4A 3J3, Québec, Canada

    Alex C. Varghese

  3. Center for Reproductive Medicine, Department of Urology, Cleveland Clinic, Euclid Avenue 9500, Cleveland, 44195, Ohio, USA

    Ashok Agarwal

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Ranoux, C. (2012). In Vivo Embryo Culture Device. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Practical Manual of In Vitro Fertilization. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-1780-5_19

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  • DOI: https://doi.org/10.1007/978-1-4419-1780-5_19

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4419-1779-9

  • Online ISBN: 978-1-4419-1780-5

  • eBook Packages: MedicineMedicine (R0)

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