Abstract
Nitrogen value of feeds depends on the proportion of feed nitrogen digested in the different compartments of the digestive tract, in particular the small intestine. in vivo and in sacco nitrogen digestibility of 21 hays with nitrogen content ranging from 0.76 to 3.07% N/DM was studied using the total faeces collection or MNBT. Five adult gelding (501 kg BW) were used for in vivo study. They were fed successively at maintenance experimental forages based-diet twice time a day at 8:00 and 16:30. Four adult gelding horses (487 kg BW) fitted with a permanent caecum canula were used for evaluation of digestibility of bags filled with experimental forages. They were fed at maintenance and received a ration composed of 70% hay and 30% barley in 2 equal meals a day (8:00 and 16:30). MRT of solid phase using mordanced chrome oxide, ingested at the beginning of the meal, and of bags, inserted in the stomach with a naso-oesophagal probe in the middle of the morning meal (after 1 kg of forage and before the rest of meal), was determined in a preliminary experiment using the four fistulated horses to check relative MRT of markers and bags. Then thirty nylon bags containing 200 mg of ground hay were inserted according to the same procedure. These were organised in 7 successive 4×4 latin squares where horses were fed three experimental hays and control hay during each period. Fifty two to 76% of bags were recovered in the caecum and the others in the faeces. DM digestibility in total tract measured in vivo and by MNBT were 50.8 and 51.9% respectively. True digestibility was estimated with both methods assuming that only dietary N reaching the faeces was N-NDF. Digestibility was corrected for MRT and bacterial N contamination for bags collected in the faeces. Digestibility was corrected for MRT and particle losses for bags collected in the caecum. Total tract true digestibility of N measured in vivo and by MNBT was 85.6% and 86.2% respectively. In the precaecal part in sacco true N digestibility was high 81.7%. It decreased when N-NDF/N ratio rose. The proportion of nitrogen digested in the small intestine averaged 87.6% and 75.6% when expressed in total percent digestible N intake or in percent of truly digestible N intake respectively.
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References
A.O.A.C. Association of Official Analytical Chemists, 1980. Official methods of analysis, 13th Ed, Washington, DC, USA.
Almeida, F.Q., S.C. Valdares Filho, J.L. Donzele, J.F. Coelho da Silva, A.C Queiroz, M.I. Lea and P.R. Ceco, 1999. Precaecal digestibility of amino acids in diets for horses. 16th ENPS, Raleigh, NC, USA, 274–279.
Arnold, F.F., W.C. Ellis, G.D. Potter, J.L. Kreider and K.R. Pond, 1983. Precaecal retention time of four feed fractions in ponies. 8th Equine Nutr.Phys; Symp., p. 240–242.
Bertone, A.L., P.J. Van Soest and T.S. Stashak, 1986. Digestion, faecal, and blood variables associated with extensive large colon resection in the horse. Am. J. Vet. Res. 5O, 253–258.
De Boer, G., J.J. Murphy and J.J. Kennelly, 1987. Mobile nylon bag for estimating intestinal availability of rumen undegradable protein. J Dairy Sci., 70, 977–982.
De Fombelle, A., A.L. Veiga, M. Varloud, C. Drogoul and V. Julliand, 2004. Effect of diet composition and feeding pattern on the precaecal digestibility of starches from diverse botanical origin measured with the mobile nylon bag technique in horses. J. Anim. Sci., 82, 3625–3634.
Ducharme, N.G., J.H. Burton, A.A. Van Dreumel, F.D. Horney, J.D. Baird and M. Arighi, 1987. Extensive large colon resection in the pony. 2. Digestibility studies and post-mortem findings. Can. J. Vet. Res. 51, 76–82.
Faichney, G.J., 1975. The use of markers to partition digestion within the gastro-intestinal tract of ruminants. In: Mc Donald I.W. and A.C. Warner (eds.) Proc. IV Int. Symp. Ruminant Physiol. Univ.of New England Press, Armidale, NSW, Australia, p. 277–291.
Fonnesbeck, P.V., 1969. Partitioning the nutrients of forages for horses. J. Anim. Sci. 29, 624–633.
Gibbs, P.G., G.D. Potter, G.T. Schelling, J.L. Kreider and C.L. Boyd, 1988. Digestion of hay protein in different segments of the equine digestive tract. J Anim Sci., 66, 400–406.
Glade, M.J., 1983. Nitrogen partitioning along the equine digestive tract. J. Anim. Sci. 57, 943–953.
Glade, M.J., 1984. The influence of dietary fibre digestibility on the nitrogen requirements of mature horses. J Anim Sci., 58(3), 638–645.
Grenet, E., W. Martin-Rosset and M. Chenost, 1984. Compared size and structure of plant particules in the horse and the sheep feces. Can J Anim Sci., 64, suppl, 345–346.
Hertel, J., H.J. Altman and K. Drepper, 1970; Ernahrungphysiologische Untersuchungen beim Pferd II-Rohnarh stoffuntersuchengen im magem — Darm — Trakt von Schlachtpferdenz. Tierphysiol Tierernahrg Futtermiltelk, 26, 167–170.
Hintz, HF, D.E. Hogue, E.F. Walker, J.E. Lowe and H.F. Schryver, 1971. apparent digestion in various segments of the digestive tract of ponies fed diets with varying roughage-grain ratios. J Anim Sci., 32(2), 245–248.
Hvelplund, T., 1985. Digestibility of rumen microbial protein and undegradeddietary protein estimated in the small intestine of sheep and by in sacco procedure. Acta Agric Scan, suppl 25, 132–144.
Hyslop, J.J., A.L. Tomlinson, A. Bayley and D. Cuddeford, 1998. Development of the mobile nylon bag technique to study the degradation dynamics of forage feed constituents in the whole digestive tract of equids. Proceedings of the British Society of Animal Science, BSAS, Penicuik, Midlothian, UK, p. 120.
INRA, 1990. Alimentation des chevaux. In: Martin-Rosset, W. (ed.) INRA Editions, Versailles, France, 232 pp.
Macheboeuf, D., C. Poncet, M. Jestin and W. Martin-Rosset, 1996. Use of a mobile nylon bag technique with caecum fistulated horses as an alternative method for estimating pre-caecal and total tract nitrogen digestibilities of feedstuffs. In Proceedings 47th European Association for Animal Production Meeting, Lillehammer, Norway, August, 26–29. Abstract H 4.9., p. 296. Wageningen Pers, Wageningen, The Netherlands.
Macheboeuf, D., M. Marangi, C. Poncet and W. Martin-Rosset, 1995. Study of nitrogen digestion from different hays by the mobile nylon bag technique in horses. Ann Zootech., 44, suppl, 219.
Martin-Rosset, W. and J.L. Tisserand, 2005. Evaluation and expression of protein allowances and protein value of feeds in the MADC system for the performance horse. In: Juliand, V. and W. Martin-Rosset (eds.) Nutrition of the performance horse: which system in Europe for evaluating the nutritional requirements? EAAP Publication No. 111. Wageningen Academic Publishers, Wageningen, the Netherlands, 103–140.
Martin-Rosset, W., J. Andrieu, M. Vermorel and J.P. Dulphy, 1984. Valeur nutritive des aliments chez le cheval. In: Jarrige, R. and W. Martin-Rosset (eds.) Le Cheval. INRA Publications,Versailles, France, 209–238.
Martin-Rosset, W., M. Doreau and P. Thivend, 1987. Digéstion de regimes à base de foin ou d’ensilage de maïs chez le cheval en croissance. Reprod. Nut. Dev., 27, 291–292.
Martin-Rosset, W., M. Vermorel, M. Doreau and J. Andrieu, 1994. The French horse feed evaluation systems and recommanded allowances for energy and protein. Livest. Proe. Sci., 40, 37–56.
Mason, V.C., 1979. The quantitative importance of bacterial residues in the non — dietary faecal nitrogen of sheep. I Methodologies studies. Z. Tierphysiol. Tierernahrung. U. Futtermittelkde 41, 131.
Mathieson, J., 1970.The automated estimation of chromic oxide. Proc.Nutr.Soc., 2, 30-31A, modified.
Mc Lean, B.M., J.J. Hyslop, A.C. Longland, D. Cuddeford and T. Holland’s, 1999. Development of the mobile nylon bag technique to determine the degradation kinetics of purified starch sources in the pre-caecal segment of equine digestive tract. Proceedings of the British Society of Animal Science, BSAS, Penicuik, Midlothian, UK, p. 138.
Meyer, H., G. Lindemann and M. Schmitt, 1982. Einflub unterschiedlicher mischfuttergaben pro mahlzeit auf praecaecale und postileale verdauungs vorgange beim pferd Fortschritte. In: Parey, P. (ed.) Der Tierphysiol Tierernahr, Hamburg und Berlin, Germany, Suppl, 32–39.
Meyer, H., S. Von Stein and M. Schmidt, 1985. Investigations to determine endogenous faecal and renal N losses in horses. In proceedings 9th ENSP, USA, 68–72.
Michalet -Doreau, B., R. Verité and P. Chapoutot, 1987. Méthodologie de la mesure de la dégradabilité in sacco de l’azote des aliments dans le rumen, INRA Prod. Anim. 69, 5–7.
Miraglia, N., Poncet C. and W., Martin-Rosset, 2003. Effect of feeding level, physiological status and breed on the digesta passage of forage based-diet in the horse. In Proc. 18th ENPS, East Lansing, USA, 275–280.
Moore-Colyer, M.J.S., H.J. Morrow and A.C. Longland, 2003. Mathematical modelling of digesta passage rate, mean retention time and in vivo apparent digestibility of two chop length of big bales of hays and big bale grass silage in ponies. Br. J. Nutr. 90. 109–118.
Morrow, H.J., M.J.S. Moore-Colyer and A.C. Longland, 1999. The apparent digestibility and the rate of passage of two chop length of big bales silage and hay. Proc. Of the British Society of Animal Science, Penicuik, UK, pp142.
Peyraud, J.L., C. Genest-Rulquin and R. Verite, 1988. Mesure de la digestion de l’azote des aliments dans l’intestin des ruminants par la technique des sachets mobiles. Reprod. Nutr. Dev., 28(suppl 1), 129–130.
Potter, G.D., P.G. Gibbs, R.G. Haley and C. Klendshoj. 1992. Digestion of protein in the small and large intestines of equines fed mixed diets. I Europaische Konferenz uber die Ernahrung des Pferdes, 140–143.
R Development Core Team, 2009. A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org.
Reitnour, C.M., J.P. Baker, G.E. Mitchell, J.R. Little and C.O. Little, 1969. Nitrogen digestion in different segments of the equine digestive tract. J Anim Sci., 29, 332–334.
Sauer, W.C., H. Jorgensen and R. Berzins, 1983. A modified nylon bag technique for determining apparent digestibilities of protein in feedstuffs for pigs. Can J. Anim. Sci., 63, 233–237.
Todd, L.K., W.C. Sauer, R.J. Christopherson, R.J. Coleman and W.R. Caine, 1995. The effect of level of intake on nutrient and energy digestibility and rate of feed passage in horses. J. Anim. Physiol. Anim. Nutr. 73, 140–148.
Uden, P., G.R. Rounsaville, G.R. Wiggans and P.J. Van Soest, 1982. The measurement of liquid and solid digesta retention in ruminants, equines and rabbits given timothy hay. Br. J. Nutr. 48, 329–339.
Van der Noot, G.W., L.D. Symons, R.K. Lydman and P.V. Fonnesbeck, 1967. Rate of passage of various feedstuffs through the digestive tract of horses. J. Anim. Sc. 26, 1309–1311.
Van Soest, P. J. and R.H. Wine, 1967. Use of detergents in the analysis of fibrous feeds IV Determination of plant Cell- Wall constituents. J. AOAC, 50(1), 50–55.
Van Weyenberg, S., J. Sales and G. Janssens, 2006. Passage rate of digesta through the equine gastrointestinal tract: a review. Livest. Sc. 99, 3–12.
Varloud, M., A. De Fombelle, A.G. Goachet, C. Drogoul and V. Julliand, 2004. Partial and total apparent digestibility of dietary carbohydrates in horses affected by the diet. J. Anim. Sci. 79, 61–72.
Wolter, R., A. Durix and J.C. Letourneau, 1974. Influence du mode de presentation du fourrage sur la vitesse du transit digestif chez le poney. Ann. Zootech., 23(3), 293–300.
Wolter, R., and D. Gouy, 1976. Etude experimentale de la digestion chez les equides par analyse du contenu digestif apres abattage. Revue Med. Vet. 127, 1723–1736.
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Martin-Rosset, W., Macheboeuf, D., Poncet, C., Jestin, M. (2012). Nitrogen digestion of a large range of hays by mobile nylon bag technique (MNBT) in horses. In: Saastamoinen, M., Fradinho, M.J., Santos, A.S., Miraglia, N. (eds) Forages and grazing in horse nutrition. Forages and grazing in horse nutrition, vol 132. Wageningen Academic Publishers, Wageningen. https://doi.org/10.3920/978-90-8686-755-4_11
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