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Handbook of hair in health and disease pp 180–196Cite as

Plucked hair: how to get stem cells and induced pluripotent stem cells for future clinical applications

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Part of the book series: Human Health Handbooks no. 1 ((HHH,volume 1))

Abstract

Generation of induced pluripotent stem cells from somatic cells by the expression of defined transcription factors has created new opportunities for the development of patient-specific therapies. Thus far, the generation of iPSCs has been performed from a variety of somatic sources. However, keratinocytes from either skin biopsies or plucked hair have shown significantly higher reprogramming efficiencies when compared to other easily accessible patient samples (e.g. a 100-fold higher reprogramming efficiency than fibroblasts). In this chapter we will summarize what we have learned after many years of intense research in both the hair follicle stem cell (SC) and iPSCs fields. First, we will focus on reviewing the current knowledge of hair follicle as a source of adult SCs for cell therapy. Second, we will discuss the future challenges in utilizing hair follicle SCs for regenerative medicine. Third, we will consider the benefits of utilizing iPSCs for cell therapy. Finally, we will provide a detailed a step-by-step protocol for the generation of human iPSCs from keratinocytes derived from a single plucked hair sample.

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Abbreviations

cMyc:

Avian myelocytomatosis viral oncogene homolog

CFE:

Colony formation efficiency

ES:

Embryonic stem

ESCs:

Embryonic stem cells

FACS:

Fluorescence activated cell-sorting

Fbx15:

F-box 15

hESC:

Human embryonic stem cells

H2B-GFP:

Histone 2B-green fluorescent protein

HMG:

High motility group

ICM:

Inner cell mass

iPSCs:

Induced pluripotent stem cells

IRS:

Inner root sheath

K15:

Cytokeratin 15

KiPSCs:

Human iPSCs derived from keratinocytes

Klf4:

Krüppel-like factor 4

Lgr5:

Leucine-rich repeat-containing G protein-coupled receptor 5

LRC:

Labelling retaining cell

MEFs:

Mouse embryonic fibroblasts

Oct4:

Octamer-binding transcription factor 4

ORS:

Outer root sheath

SC:

Stem cell

Sox2:

Sry-related HMG box 2

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Authors and Affiliations

  1. Center of Regenerative Medicine of Barcelona (CMR[B]), Doctor Aiguader 88, p7, 08003, Barcelona, Spain

    Núria Montserrat & Juan Carlos Izpisúa Belmonte

  2. Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA

    Juan Carlos Izpisúa Belmonte

Authors
  1. Núria Montserrat
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  2. Juan Carlos Izpisúa Belmonte
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Editor information

Victor R. Preedy PhD DSc FRSPH FIBiol FRCPath

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© 2012 Wageningen Academic Publishers

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Montserrat, N., Belmonte, J.C.I. (2012). Plucked hair: how to get stem cells and induced pluripotent stem cells for future clinical applications. In: Preedy, V.R. (eds) Handbook of hair in health and disease. Human Health Handbooks no. 1, vol 1. Wageningen Academic Publishers. https://doi.org/10.3920/978-90-8686-728-8_9

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