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Hepatitis C

  • D. Robert Dufour

Abstract

Hepatitis C virus (HCV) is the most common chronic viral infection in North America and Europe and a common infection worldwide. In the United States Third National Health and Nutrition Examination Survey, it was estimated that 3.9 million people had detectable antibodies to HCV, and 75% of them were positive for HCV RNA (1). This survey included only individuals living in households. There is evidence that HCV is much more common in certain populations, such as the homeless and prison inmates, where prevalence might be as high as 40% (2). The prevalence of HCV is also higher among veterans seeking care in VA medical centers; nationwide, the prevalence is just under 7% (3), with a prevalence over 10% in urban hospitals (4). Worldwide, an estimated 170 million individuals are chronically infected with HCV (5). The age of infected individuals differs significantly in different areas of the world. In Australia, North America, and much of Europe, infections seems to peak in persons currently between the ages of 40 and 60, whereas in Japan, Italy, and Egypt, prevalence of HCV infection increases steadily with increasing age (6). Males are more likely to be infected than females (1). The prevalence of acute infections with HCV has fallen by 80% over the past decade (7).

There are a number of recognized risk factors for HCV. The most common are injection drug use and transfusion or transplantation before 1992 (8). Although the overwhelming majority of those using injection drugs in the 1960s and 1970s became infected with HCV (9), the likelihood of infection among current, younger injecting drug users is only about 50% (10), this reflects an overall decline in the incidence of acute HCV infection among injecting drug users (7). Even among blood donors who initially deny risk factors but are found to be HCV positive, a history of injection drug use on follow-up questioning is 50 times more likely than in donors who are HCV negative (11). Less commonly, HCV is transmitted by dialysis(12,13), by needle-stick injury (14), and by vertical transmission from an infected mother to her child (15–17); the likelihood of infection with the last two of these routes is approx 3–5%. The role of sexual transmission is controversial, but evidence suggests that having multiple sexual partners increases risk of transmission of HCV (18–21); the likelihood of infection in monogamous sex partners of HCV-infected individuals is similar to that in the population as a whole, however (22,23).

Keywords

Sustain Virologic Response Early Virologic Response Iral Load 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Alter, M., Kruszon-Moran, D., Nainan, O., et al. The prevalence of hepatitis C virus infection in the United States, 1988 through 1994. N. Engl. J. Med. 341:556–562, 1999.PubMedCrossRefGoogle Scholar
  2. 2.
    Kim, W. The burden of hepatitis C in the United States. Hepatology 36:S30–S34, 2002.PubMedCrossRefGoogle Scholar
  3. 3.
    Ho, S., Gebhard, R., Tetrick, L., et al. Key components and logistics of hepatitis C care within a VISN: a proposal. Veterans Health Syst. J. 41–45, 1999.Google Scholar
  4. 4.
    Brau, N., Bini, E., Shahidi, A., et al. Prevalence of hepatitis C and coinfection with HIV among United States veterans in the New Yo r k City metropolitan area. Am. J. Gastroenterol. 97:2071–2078, 2002.PubMedGoogle Scholar
  5. 5.
    Mansell, C. and Locarnini, S. Epidemiology of hepatitis C in the East. Semin. Liver Dis. 15:15–32, 1995.PubMedCrossRefGoogle Scholar
  6. 6.
    Wasley, A. and Alter, M. Epidemiology of hepatitis C: geographic differences and temporal trends. Semin. Liver Dis. 20:1–16, 2000.PubMedCrossRefGoogle Scholar
  7. 7.
    Alter, M. Epidemiology of hepatitis C. Hepatology 26:62S–65S, 1997.PubMedCrossRefGoogle Scholar
  8. 8.
    Centers for Disease Control and Prevention. Recommendations for the prevention and control of hepatitis C virus (HCV) infection and HCV-related chronic disease. MMWR 47: 1–39, 1998.Google Scholar
  9. 9.
    McCarthy, J. and Flynn, N. Hepatitis C in methadone maintenance patients: prevalence and public policy implications. J. Addict. Dis. 20:19–31, 2001.PubMedCrossRefGoogle Scholar
  10. 10.
    Miller, C., Johnston, C., Spittal, P., et al. Opportunities for prevention: hepatitis C prevalence and incidence in a cohort of young injection drug users. Hepatology 36:737–742, 2002.PubMedCrossRefGoogle Scholar
  11. 11.
    Murphy, E., Bryzman, S., Glynn, S., et al. Risk factors for hepatitis C virus infection in United States blood donors. Hepatology 31: 756–762, 2002.CrossRefGoogle Scholar
  12. 12.
    Bukh, J., Wantzin, P., Krogsgaard, K., Knudsen, F., Purcell, R., and Miller, R. High prevalence of hepatitis C virus (HCV) RNA in dialysis patients: failure of commercially available antibody tests to identify a significant number of patients with HCV infection. Copenhagen Dialysis HCV Study Group. J. Infect. Dis. 168: 1343–1348, 1993.PubMedGoogle Scholar
  13. 13.
    Courouce, A., Le Marrec, N., Girault, A., Ducamp, S., and Simon, N. Anti-hepatitis C virus (anti-HCV) seroconversion in patients undergoing hemodialysis: comparison of second- and third-generation anti-HCV assays. Transfusion 34:790–795, 1994.PubMedCrossRefGoogle Scholar
  14. 14.
    Mitsui, T., Iwano, K., Masuko, K., et al. Hepatitis C virus infection in medical personnel after needlestick accident. Hepatology 16: 1109–1114, 1992.PubMedCrossRefGoogle Scholar
  15. 15.
    Okamoto, M., Nagata, I., Murakami, J., et al. Prospective reevaluation of risk factors in mother-to-child transmission of hepatitis C virus: high virus load, vaginal delivery, and negative anti-NS4 antibody. J. Infect. Dis. 182:1511–1514, 2000.PubMedCrossRefGoogle Scholar
  16. 16.
    Mazza, C., Ravaggi, A., Rodella, A., et al. Prospective study of mother-to-infant transmission of hepatitis C virus (HCV) infection. Study Group for Vertical Transmission. J. Med. Virol. 54:12–19, 1998.PubMedCrossRefGoogle Scholar
  17. 17.
    Roberts, E. and Yeung, L. Maternal-infant transmission of hepatitis C virus infection. Hepatology 36:S106–S112, 2002.PubMedCrossRefGoogle Scholar
  18. 18.
    Kao, J., Hwang, Y., Chen, P., et al. Transmission of hepatitis C virus between spouses: the important role of exposure duration. Am. J. Gastroenterol. 91:2087–2090, 1996.PubMedGoogle Scholar
  19. 19.
    Osella, A., Massa, M., Joekes, S., et al. Hepatitis B and C virus sexual transmission among homosexual men. Am. J. Gastroenterol. 93: 49–52, 1998.PubMedCrossRefGoogle Scholar
  20. 20.
    Terrault, N. Sexual activity as a risk factor for hepatitis C. Hepatology 36:S99–S105, 2002.PubMedCrossRefGoogle Scholar
  21. 21.
    Wejstal, R. Sexual transmission of hepatitis C virus. J. Hepatol. 31 (Suppl. 1):92–95, 1995.Google Scholar
  22. 22.
    Bresters, D., Mauser-Bunschoten, E., Reesink, et al. Sexual transmission of hepatitis C virus. Lancet 342:210–211, 1993.PubMedCrossRefGoogle Scholar
  23. 23.
    Conry-Cantilena, C., Van Raden, M., Gibble, J., et al. Routes of infection, viremia, and liver disease in blood donors found to have hepatitis C virus infection. N. Engl. J. Med. 334:1691–1696, 1996.PubMedCrossRefGoogle Scholar
  24. 24.
    Choo, Q.-L., Kuo, G., Weiner, A., Bradley, D., and Houghton, M. Isolation of a c-DNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362, 1989.PubMedCrossRefGoogle Scholar
  25. 25.
    Houghton, M., Weiner, A., Han, J., Kuo, G., and Choo, Q. Molecular biology of the hepatitis C viruses: implications for diagnosis, development, and control of viral disease. Hepatology 14:381–388, 1991.PubMedCrossRefGoogle Scholar
  26. 26.
    Honda, M., Kaneko, S., Sakai, A., Unoura, M., Murakami, S., and Kobayashi, K. Degree of diversity of hepatitis C virus quasispecies and progression of liver disease. Hepatology 20:1144–1151, 1994.PubMedCrossRefGoogle Scholar
  27. 27.
    Baumert, T., Wellnitz, S., Aono, S., et al. Antibodies against hepatitis C virus-like particles and viral clearance in acute and chronic hepatitis C. Hepatology 32:610–617, 2000.PubMedCrossRefGoogle Scholar
  28. 28.
    Rehermann, B. Interaction between the hepatitis C virus and the immune system. Semin. Liver Dis. 20:127–144, 2000.PubMedCrossRefGoogle Scholar
  29. 29.
    Frangeul, L., Cresta, P., Perrin, M., et al. Mutations in NS5A region of hepatitis C virus genome correlate with presence of NS5A antibodies and response to interferon therapy for most common European hepatitis C virus genotypes. Hepatology 28:1674–1679, 1998.PubMedCrossRefGoogle Scholar
  30. 30.
    Ideo, G. and Bellobuono, A. New therapies for the treatment of chronic hepatitis C. Curr. Pharm. Des. 8:959–966, 2002.PubMedCrossRefGoogle Scholar
  31. 31.
    Bukh, J., Miller, R., and Purcell, R. Genetic heterogeneity of hepatitis C virus: quasispecies and genotypes. Semin. Liver. Dis. 15:41–63, 1995.PubMedCrossRefGoogle Scholar
  32. 32.
    Farci, P., Shimoda, A., Coiana, A., et al. The outcome of acute hepatitis C predicted by the evolution of the viral quasispecies. Science 288:339–344, 2000.PubMedCrossRefGoogle Scholar
  33. 33.
    Lin, H. J., Seeff, L. B., Barbosa, L., and Hollinger, F. B. Occurrence of identical hypervariable region 1 sequences of hepatitis C virus in transfusion recipients and their respective blood donors: divergence over time. Hepatology 34:424–429, 2001.PubMedCrossRefGoogle Scholar
  34. 34.
    Gao, G., Buskell, Z., Seeff, L., and Tabor, E. Drift in the hypervari-able region of the hepatitis C virus during 27 years in two patients. J. Med. Virol. 68:60–67, 2002.PubMedCrossRefGoogle Scholar
  35. 35.
    El-Awady, M., Ismail, S., El-Sagheer, M., Sabour, Y., Amr, K., and Zaki, E. Assay for hepatitis C virus in peripheral blood mononuclear cells enhances sensitivity of diagnosis and monitoring of HCV-associated hepatitis. Clin. Chim. Acta. 283:1–14, 1999.PubMedCrossRefGoogle Scholar
  36. 36.
    Naito, M., Hayashi, N., Hagiwara, H., et al. Serial quantitative analysis of serum hepatitis C virus RNA level in patients with acute and chronic hepatitis C. J. Hepatol. 20:755–759, 1994.PubMedCrossRefGoogle Scholar
  37. 37.
    Seeff, L., Wright, E., Zimmerman, H., and McCollum, R. W. VA cooperative study of post-transfusion hepatitis, 1969–1974: incidence and characteristics of hepatitis and responsible risk factors. Am. J. Med. Sci. 270:355–362, 1975.PubMedCrossRefGoogle Scholar
  38. 38.
    Healey, C., Sabharwal, N., Daub, J., et al. Outbreak of acute hepatitis C following the use of anti-hepatitis C virus—–screened intravenous immunoglobulin therapy. Gastroenterology 110:1120–1126, 1996.PubMedCrossRefGoogle Scholar
  39. 39.
    Hino, K., Sainokami, S., Shimoda, K., Niwa, H., and Iino, S. Clinical course of acute hepatitis C and changes in HCV markers. Dig. Dis. Sci. 39:19–27, 1994.PubMedCrossRefGoogle Scholar
  40. 40.
    Giuberti, T., Marin, M., Ferrari, C., et al. Hepatitis C virus viremia following clinical resolution of acute hepatitis C. J. Hepatol. 20: 666–671, 1994.PubMedCrossRefGoogle Scholar
  41. 41.
    Villano, S., Vlahov, D., Nelson, K., Cohn, S., and Thomas, D. Persistence of viremia and the importance of long-term follow-up after acute hepatitis C infection. Hepatology 29:908–914, 1999.PubMedCrossRefGoogle Scholar
  42. 42.
    Datz, C., Cramp, M., Haas, T., et al. The natural course of hepatitis C virus infection 18 years after an epidemic outbreak of non-A, non-B hepatitis in a plasmapheresis centre. Gut. 44:563–567, 1999.PubMedCrossRefGoogle Scholar
  43. 43.
    Alter, H. and Seeff, L. Recovery, persistence, and sequelae in hepatitis C virus infection: a perspective on long-term outcome. Semin. Liver Dis. 20:17–35, 2000.PubMedCrossRefGoogle Scholar
  44. 44.
    Gholson, C., Morgan, K., Catinis, G., et al. Chronic hepatitis C with normal aminotransferase levels: a clinical histologic study. Am. J. Gastroenterol. 92:1788–1792, 1997.PubMedGoogle Scholar
  45. 45.
    Dincer, D., Okten, A., Kaymakoglu, S., et al. Persistently normal alanine transaminase levels in chronic C hepatitis: what does it tell us? Hepatogastroenterology 48:1397–1400, 2001.PubMedGoogle Scholar
  46. 46.
    Pradat, P., Alberti, A., Poynard, T., et al. Predictive value of ALT levels for histologic findings in chronic hepatitis C: a European collaborative study. Hepatology 36:973–977, 2002.PubMedGoogle Scholar
  47. 47.
    McCormick, S., Goodman, Z., Maydonovitch, C., and Sjogren, M. Evaluation of liver histology, ALT elevation, and HCV RNA titer in patients with chronic hepatitis C. Am. J. Gastroenterol. 91:1516– 1522, 1996.PubMedGoogle Scholar
  48. 48.
    Dufour, M., Stinson, F., and Caces, M. Trends in cirrhosis morbidity and mortality: United States, 1979–1988. Semin. Liver Dis. 13:109–125, 1993.PubMedCrossRefGoogle Scholar
  49. 49.
    Alberti, A., Chemello, L., and Benvegnu, L. Natural history of hepatitis C. J. Hepatol. 31(Suppl. 1):17–24, 1999.PubMedCrossRefGoogle Scholar
  50. 50.
    Seeff, L. Natural history of chronic hepatitis C. Hepatology 36:S35– S46, 2002.PubMedCrossRefGoogle Scholar
  51. 51.
    Vogt, M., Lang, T., Frosner, G., et al. Prevalence and clinical outcome of hepatitis C infection in children who underwent cardiac surgery before the implementation of blood-donor screening. N. Engl. J. Med. 341:866–870, 1999.PubMedCrossRefGoogle Scholar
  52. 52.
    Kenny-Walsh, E. Clinical outcomes after hepatitis C infection from contaminated anti-D immune globulin. N. Engl. J. Med. 340:1228– 1233, 1999.PubMedCrossRefGoogle Scholar
  53. 53.
    Wiese, M., Berr, F., Lafrenz, M., Porst, H., and Oesen, U. Low frequency of cirrhosis in a hepatitis C (genotype 1b) single-source outbreak in Germany: a 20-year multicenter study. Hepatology 32: 91–96, 2000.PubMedCrossRefGoogle Scholar
  54. 54.
    Fattovich, G., Giustina, G., Degos, F., et al. Morbidity and mortality in compensated cirrhosis type C: a restrospective follow-up study of 384 patients. Gastroenterology 112:463–472, 1997.PubMedCrossRefGoogle Scholar
  55. 55.
    El-Serag, H. and Mason, A. Rising incidence of hepatocellular carcinoma in the United States. N. Engl. J. Med. 340:745–750,1999.PubMedCrossRefGoogle Scholar
  56. 56.
    Davis, G., Albright, J., Cook, S., et al. Projecting the future healthcare burden from hepatitis C in the United States. Hepatology 28:390a, 1998.CrossRefGoogle Scholar
  57. 57.
    El-Serag, H. Epidemiology of hepatocellular carcinoma. Clin. Liver Dis. 5:87–107, 2001.PubMedCrossRefGoogle Scholar
  58. 58.
    Yang, H., Lu, S., Liaw, Y., et al. Hepatitis B e antigen and the risk of hepatocellular carcinoma. N. Engl. J. Med. 347:168–174, 2002.PubMedCrossRefGoogle Scholar
  59. 59.
    Di Bisceglie, A. Hepatitis C and hepatocellular carcinoma. Hepatology 26:34S–38S, 1997.PubMedCrossRefGoogle Scholar
  60. 60.
    Fattovich, G. Progression of hepatitis B and C to hepatocellular carcinoma in Western countries. Hepatogastroenterology 45(Suppl. 3): 1206–1213, 1998.PubMedGoogle Scholar
  61. 61.
    El-Serag, H., Hampel, H., Yeh, C., and Rabeneck, L. Extrahepatic manifestations of hepatitis C among United States male veterans. Hepatology 36:1439–1445, 2002.PubMedGoogle Scholar
  62. 62.
    Kayali, Z., Buckwold, V., Zimmerman, B., and Schmidt, W. Hepatitis C, cryoglobulinemia, and cirrhosis: a meta-analysis. Hepatology 36:978–985, 2002.PubMedGoogle Scholar
  63. 63.
    Hermine, O., Lefrere, F., Bronowicki, J., et al. Regression of splenic lymphoma with villous lymphocytes after treatment of hepatitis C virus infection. N. Engl. J. Med. 347:89–94, 2002.PubMedCrossRefGoogle Scholar
  64. 64.
    Mehta, S., Brancati, F., Sulkowski, M., Strathdee, S., Szklo, M., and Thomas, D. Prevalence of type 2 diabetes mellitus among persons with hepatitis C virus infection in the United States. Ann. Intern. Med. 133:592–599, 2000.PubMedGoogle Scholar
  65. 65.
    Dufour, D., Talastas, M., Fernandez, M., Harris, B., Strader, D., and Seeff, L. Low positive anti-hepatitis C virus enzyme immunoassay results: an important predictor of low likelihood of hepatitis C infection. Clin. Chem. 49:479–486, 2003.PubMedCrossRefGoogle Scholar
  66. 66.
    Alter, M., Kuhnert, W., and Finelli, L. Guidelines for laboratory testing and results reporting of antibody to hepatitis C virus. MMWR 52:1–15, 2003.PubMedGoogle Scholar
  67. 67.
    Marcellin, P., Martinot-Peignoux, M., Elias., et al. Hepatitis C virus (HCV) viremia in human immunodeficiency virus-seronegative and -seropositive patients with indeterminate HCV recombinant immunoblot assay. J. Infect. Dis. 170:433–435, 1994.PubMedGoogle Scholar
  68. 68.
    Gretch, D. Diagnostic tests for hepatitis C. Hepatology 26: 43S–47S, 1997.PubMedCrossRefGoogle Scholar
  69. 69.
    Barrera, J., Bruguera, M., Ercilla, M., et al. Persistent hepatitis C viremia after acute self-limiting posttransfusion hepatitis C. Hepatology 21:639–644, 1995.PubMedCrossRefGoogle Scholar
  70. 70.
    Courouce, A., Noel, L., Barin, F., et al. A comparative evaluation of the sensitivity of five anti-hepatitis C virus immunoblot assays. Vox Sang 74:217–224, 1998.PubMedCrossRefGoogle Scholar
  71. 71.
    Damen, M., Zaaijer, H., Cuypers, H., et al. Reliability of the third-generation recombinant immunoblot assay for hepatitis C virus. Transfusion 35:745–749, 1995.PubMedCrossRefGoogle Scholar
  72. 72.
    Vernelen, K., Claeys, H., Verhaert, H., Volckaerts, A., and Vernylen, C. Significance of NS3 and NS5 antigens in screening for HCV antibody. Lancet 343:853, 1994.PubMedCrossRefGoogle Scholar
  73. 73.
    LaPerche, S., Courouce, A., Lemaire, J., Coste, J., Defer, C., and Cantaloube, J. GB virus type C/hepatitis G virus infection in French blood donors with anti-NS5 isolated reactivities by recombinant immunoblot assay for hepatitis C virus. Transfusion 39:790–791, 1999.PubMedCrossRefGoogle Scholar
  74. 74.
    Aoyagi, K., Ohue, C., Iida, K., et al. Development of a simple and highly sensitive enzyme immunoassay for hepatitis C virus core antigen. J. Clin. Microbiol. 37:1802–1808, 1999.PubMedGoogle Scholar
  75. 75.
    Peterson, J., Green, G., Iida, K., et al. Detection of hepatitis C core antigen in the antibody negative ‘window’ phase of hepatitis C infection. Vox Sang 78:80–85, 2000.PubMedCrossRefGoogle Scholar
  76. 76.
    Bouvier-Alias, M., Patel, K., Dahari, H., et al. Clinical utility of total HCV core antigen quantification: a new indirect marker of HCV replication. Hepatology 36:211–218, 2002.PubMedCrossRefGoogle Scholar
  77. 77.
    Kurtz, J., Boxall, E., Qusir, N., Shirley, J., Coleman, D., and Chandler, C. The diagnostic significance of an assay for total’ hepatitis C core antigen. J. Virol. Methods. 96:127–132, 2001.PubMedCrossRefGoogle Scholar
  78. 78.
    Tanaka, E., Ohue, C., Aoyagi, K., et al. Evaluation of a new enzyme immunoassay for hepatitis C virus (HCV) core antigen with clinical sensitivity approximating that of genomic amplification of HCV RNA. Hepatology 32:388–393, 2000.PubMedCrossRefGoogle Scholar
  79. 79.
    Kobayashi, M., Tanaka, E., Matsumoto, A., et al. Clinical application of hepatitis C virus core protein in early diagnosis of acute hepatitis C. J. Gastroenterol. 33:508–511, 1998.PubMedCrossRefGoogle Scholar
  80. 80.
    Tan, D., Im, S., Peng, W., and Ng, M. Follow-up study of acute hepatitis C. Arch. Virol. 138:71–84, 1994.PubMedCrossRefGoogle Scholar
  81. 81.
    Nguyen, T., Sedghi-Vaziri, A., Wilkes, L., et al. Fluctuations in viral load (HCV RNA) are relatively insignificant in untreated patients with chronic HCV infection. J. Viral Hepat. 3:75–78, 1996.PubMedCrossRefGoogle Scholar
  82. 82.
    Fanning, L., Kenny-Walsh, E., Levis, J., et al. Natural fluctuations of hepatitis C viral load in a homogeneous patient population: a prospective study. Hepatology 31:225–229, 2000.PubMedCrossRefGoogle Scholar
  83. 83.
    Yokosuka, O., Kojima, H., Imazeki, F., et al. Spontaneous negativa-tion of serum hepatitis C virus RNA is a rare event in type C chronic liver diseases: analysis of HCV RNA in 320 patients who were followed for more than 3 years. J. Hepatol. 31:394–399, 1999.PubMedCrossRefGoogle Scholar
  84. 84.
    Davis, G., Lau, J., Urdea, M., et al. Quantitative detection of hepatitis C virus RNA with a solid-phase signal amplification method: definition of optimal conditions for specimen collection and clinical application in interferon-treated patients. Hepatology 19:1337–1341, 1994.PubMedCrossRefGoogle Scholar
  85. 85.
    Grant, P., Kitchen, A., Barbara, J., et al. Effects of handling and storage of blood on the stability of hepatitis C virus RNA: implications for NAT testing in transfusion practice. Vox Sang 78:137–142, 2000.PubMedCrossRefGoogle Scholar
  86. 86.
    Trabaud, M., Bailly, F., Colucci, G., and Trepo, C. Stability of hepatitis C virus RNA in serum from samples collected in a closed-tube system for serum separation and transport, as measured by a quantitative competitive PCR assay. J. Viral. Hepat. 3:207–209, 1996CrossRefGoogle Scholar
  87. 87.
    Doglio, A., Laffont, C., Caroli-Bosc, F., Rochet, P., and Lefebvre, J. Second generation of the automated Cobas Amplicor HCV assay improves sensitivity of hepatitis C virus RNA detection and yields results that are more clinically relevant. J. Clin. Microbiol. 37:1567–1569, 1999.PubMedGoogle Scholar
  88. 88.
    Comanor, L., Anderson, F., Ghany, M., et al. Transcription-mediated amplification is more sensitive than conventional PCR-based assays for detecting residual serum HCV RNA at end of treatment. Am. J. Gastroenterol. 96:2968–2972, 2001.PubMedCrossRefGoogle Scholar
  89. 89.
    Sarrazin, C., Teuber, G., Kokka, R., Rabenau, H., and Zeuzum, S. Detection of residual hepatitis C virus RNA by transcription-mediated amplification in patients with complete virologic response according to polymarase chain reaction-based assays. Hepatology 32:818–823, 2000.PubMedCrossRefGoogle Scholar
  90. 90.
    Ross, R., Viazov, S., Hoffmann, S., and Roggendorf, M. Performance characteristics of a transcription-mediated nucleic acid amplification assay for qualitative detection of hepatitis C virus RNA. J. Clin. Lab. Anal. 15:308–313, 2001.PubMedCrossRefGoogle Scholar
  91. 91.
    Krajden, M., Ziermann, R., Khan, A., et al. Qualitative detection of hepatitis C virus RNA: comparison of analytical sensitivity, clinical performance, and workflow of the Cobas Amplicor HCV Test Version 2.0 and the HCV RNA Transcription-Mediated Amplification Qualitative Assay. J. Clin. Microbiol. 40:2903–2907, 2002.PubMedCrossRefGoogle Scholar
  92. 92.
    Saldanha, J., Lelie, N., and Heath, A. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA. WHO Collaborative Study Group. Vox Sang 76:149–158, 1999.PubMedCrossRefGoogle Scholar
  93. 93.
    Ross, R., Viazov, S., Sarr, S., Hoffmann, S., Kramer, A., and Roggendorf, M. Quantitation of hepatitis C virus RNA by third generation branched DNA-based signal amplification assay. J. Virol. Methods 101:159–168, 2002.PubMedCrossRefGoogle Scholar
  94. 94.
    Sherman, K., Rouster, S., and Horn, P. Comparison of methodologies for quantification of hepatitis C virus (HCV) RNA in patients coinfected with HCV and human immunodeficiency virus. Clin. Infect. Dis. 35:482–487, 2002.PubMedCrossRefGoogle Scholar
  95. 95.
    Afonso, A., Didier, J., Plouvier, E., et al. Performance of an automated system for quantification of hepatitis C virus RNA. J. Virol. Methods 86:55–60, 2000.CrossRefGoogle Scholar
  96. 96.
    Trimoulet, P., Halfon, P., Pohier, E., Khiri, H., Chene, G., and Fleury, H. Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum. J. Clin. Microbiol. 40:2031–2036, 2002.PubMedCrossRefGoogle Scholar
  97. 97.
    Germer, J., Heimgartner, P., Ilstrup, D., Harmsen, W., Jenkins, G., and Patel, R. Comparative evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR Version 2.0 assays for quantification of hepatitis C virus RNA in serum. J. Clin. Microbiol. 40:495–500, 2002.PubMedCrossRefGoogle Scholar
  98. 98.
    Pawlotsky, J. Molecular diagnosis of viral hepatitis. Gastroenterology 122:1554–1568, 2002.PubMedCrossRefGoogle Scholar
  99. 99.
    Yang, J., Lai, J., Douglas, S., Metzger, D., Zhu, X., and Ho, W. Real-time RT-PCR for quantitation of hepatitis C virus RNA. J. Virol. Methods 102:119–128, 2002.PubMedCrossRefGoogle Scholar
  100. 100.
    van Doorn, L., Kleter, B., Stuyver, L., et al. Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profiles. J. Hepatol. 21:122–129, 1994.PubMedCrossRefGoogle Scholar
  101. 101.
    Stuyver, L., Wyseur, A., Arnhem, W. V., et al. Hepatitis C genotyping by means of 5’-UR/core line probe assays and molecular analysis of untypeable samples. Virus Res. 38:137–157, 1995.PubMedCrossRefGoogle Scholar
  102. 102.
    Mitchell, P., Sloan, L., Majewski, D., et al. Comparison of line probe assay and DNA sequencing of 5’ untranslated region for genotyping hepatitis C virus: description of novel line probe patterns. Diagn. Microbiol. Infect. Dis. 42:175–179, 2002.PubMedCrossRefGoogle Scholar
  103. 103.
    Pawlotsky, J., Prescott, L., Simmonds, P., et al. Serological determination of hepatitis C virus genotype: comparison with a standardized genotyping assay. J. Clin. Microbiol. 35:1734–1739, 1997.PubMedGoogle Scholar
  104. 104.
    Fabrizi, F., Martin, P., Quan, S., et al. Serotyping strip immunoblot assay for assessing hepatitis C virus strains in dialysis patients. Am. J. Kidney Dis. 35:832–838, 2000.PubMedCrossRefGoogle Scholar
  105. 105.
    Toyoda, H., Fukuda, Y., Hayakawa, T., et al. Presence of multiple genotype-specific antibodies in patients with persistent infection with hepatitis C virus (HCV) of a single genotype: evidence for transient or occult superinfection with HCV of different genotypes. Am. J. Gastroenterol. 94:2230–2236, 1999.PubMedCrossRefGoogle Scholar
  106. 106.
    Dufour, D., Lott, J., Nolte, F., Gretch, D., Koff, R., and Seeff, L. Diagnosis and monitoring of hepatic injury. I. Performance characteristics of laboratory tests. Clin. Chem. 46:2027–2049, 2000.PubMedGoogle Scholar
  107. 107.
    Sakuma, I., Enomoto, N., Kurosaki, M., Izumi, N., Marumo, F., and Sato, C. Differential effect of interferon on hepatitis C virus 1b qua-sispecies in the nonstructural protein 5A gene. J. Infect. Dis. 180:1001–1009, 1999.PubMedCrossRefGoogle Scholar
  108. 108.
    Murakami, T., Enomoto, N., Kurosaki, M., Izumi, N., Marumo, F., and Sato, C. Mutations in nonstructural protein 5A gene and response to interferon in hepatitis C virus genotype 2 infection. Hepatology 30:1045–1053, 1999.PubMedCrossRefGoogle Scholar
  109. 109.
    Terazawa, Y., Yoshioka, K., Kobayashi, M., et al. Mutations in inter-feron sensitivity-determining region of hepatitis C virus: its relation to change in viral load. Am. J. Gastroenterol 95:1781–1787, 2000.PubMedGoogle Scholar
  110. 110.
    Sarrazin, C., Berg, T., Lee, J., et al. Mutations in the protein kinase-binding domain of the NS5A protein in patients infected with hepatitis C virus type 1a are associated with treatment response. J. Infect. Dis. 181:432–441, 2000.PubMedCrossRefGoogle Scholar
  111. 111.
    Murphy, M., Rosen, H., Marousek, G., and Chou, S. Analysis of sequence configurations of the ISDR, PKR-binding domain, and V 3 region as predictors of response to induction interferon-alpha and ribavirin therapy in chronic hepatitis C infection. Dig. Dis. Sci. 47:1195–1205, 2002.PubMedCrossRefGoogle Scholar
  112. 112.
    Saiz, J., Lopez-Labrador, F., Ampurdanes, S., et al. The prognostic relevance of the nonstructural 5A gene interferon sensitivity determining region is different in infections with genotype 1b and 3a isolates of hepatitis C virus. J. Infect. Dis. 177:839–847, 1998.PubMedGoogle Scholar
  113. 113.
    Nakano, I., Fukuda, Y., Katano, Y., Nakano, S., Kumada, T., and Hayakawa, T. Why is the interferon sensitivity-determining region (ISDR) system useful in Japan? J. Hepatol. 30:1014–1022, 1999.PubMedCrossRefGoogle Scholar
  114. 114.
    Alberti, A., Boccato, S., Vario, A., and Benvegnu, L. Therapy of acute hepatitis C. Hepatology 36:S195–S200, 2002.PubMedCrossRefGoogle Scholar
  115. 115.
    Beld, M., Penning, M., Putten, M. V., et al. Quantitative antibody responses to structural (core) and nonstructural (NS3, NS4, and NS5) hepatitis C virus proteins among seroconverting injecting drug users: impact of epitope variation and relationship to detection of HCV RNA in blood. Hepatology 29:1288–1298, 1999.PubMedCrossRefGoogle Scholar
  116. 116.
    Rodger, A., Roberts, S., Lanigan, A., Bowden, S., Brown, T., and Crofts, N. Assessment of long-term outcomes of community-acquired hepatitis C infection in a cohort with sera stored from 1971 to 1975. Hepatology 32:582–587, 2000.PubMedCrossRefGoogle Scholar
  117. 117.
    Seeff, L., Hollinger, F., Alter, H., et al. Long-term mortality and morbidity of transfusion-associated non-A, non-B, and type C hepatitis: A National Heart, Lung, and Blood Institute collaborative study. Hepatology 33:455–463, 2001.PubMedCrossRefGoogle Scholar
  118. 118.
    Pawlotsky, J. Use and interpretation of virological tests for hepatitis C. Hepatology 36:S65–S73, 2002.PubMedCrossRefGoogle Scholar
  119. 119.
    Bodenheimer, H., Lindsay, K., Davis, G., Lewis, J., Thung, S., and Seeff, L. Tolerance and efficacy of oral ribavirin treatment of chronic hepatitis C: a multicenter trial. Hepatology 26:473–477, 1997.PubMedCrossRefGoogle Scholar
  120. 120.
    Cummings, K., Lee, S., West, E., et al. Interferon and ribavirin vs interferon alone in the re-treatment of chronic hepatitis C previously nonresponsive to interferon: A meta-analysis of randomized trials. JAMA 285:193–199, 2001.PubMedCrossRefGoogle Scholar
  121. 121.
    Di Bisceglie, A. and Hoofnagle, J. Optimal therapy of hepatitis C. Hepatology 36:S121–S127, 2002.PubMedGoogle Scholar
  122. 122.
    Schalm, S., Fattovich, G., and Brouwer, J. Therapy of hepatitis C: patients with cirrhosis. Hepatology 26:128S–132S, 1997.PubMedCrossRefGoogle Scholar
  123. 123.
    Wright, T. Treatment of patients with hepatitis C and cirrhosis. Hepatology 36:S185–S194, 2002.PubMedCrossRefGoogle Scholar
  124. 124.
    Layden, T., Mika, B., and Wiley, T. Hepatitis C kinetics: mathematical modeling of viral response to therapy. Semin. Liver Dis. 20:173–183, 2000.PubMedCrossRefGoogle Scholar
  125. 125.
    Davis, G. Monitoring of viral levels during therapy of hepatitis C. Hepatology 36:S145–S151, 2002.PubMedCrossRefGoogle Scholar
  126. 126.
    Anon. National Institutes of Health Consensus Development Conference Statement: Management of hepatitis C: 2002—June 10–12, 2002. Hepatology 36:S3–S20, 2002.Google Scholar
  127. 127.
    Dufour, J., De Lellis, R., and Kaplan, M. Regression of hepatic fibrosis in hepatitis C with long-term interferon treatment. Dig. Dis. Sci. 43:2573–2576, 1998.PubMedCrossRefGoogle Scholar
  128. 128.
    Bonino, F. Effect of interferon-α on progression of cirrhosis to hepa-tocellular carcinoma: A retrospective cohort study. Lancet 351:1535– 1539, 1998.CrossRefGoogle Scholar
  129. 129.
    Galel, S., Strong, D., Tegtmeier, G., et al. Comparative yield of HCV RNA testing in blood donors screened by 2.0 versus 3.0 antibody assays. Transfusion 42:1507–1513, 2002.PubMedCrossRefGoogle Scholar
  130. 130.
    Hitzler, W. and Runkel, S. Screening of blood donations by hepatitis C virus polymerase chain reaction (HCV-PCR) improves safety of blood products by window period reduction. Clin. Lab. 47:219–222, 2001.PubMedGoogle Scholar
  131. 131.
    Busch, M., Kleinman, S., and Nemo, G. Current and emerging infectious risks of blood transfusions. JAMA 289:959–962, 2003.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • D. Robert Dufour
    • 1
  1. 1.Pathology and Laboratory Medicine ServiceVeterans Affairs Medical CenterWashingtonUSA

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